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1thu

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(New page: 200px<br /><applet load="1thu" size="450" color="white" frame="true" align="right" spinBox="true" caption="1thu, resolution 2.6&Aring;" /> '''THE STRUCTURES OF THR...)
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[[Image:1thu.gif|left|200px]]<br /><applet load="1thu" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1thu, resolution 2.6&Aring;" />
 
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'''THE STRUCTURES OF THREE CRYSTAL FORMS OF THE SWEET PROTEIN THAUMATIN'''<br />
 
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==Overview==
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==THE STRUCTURES OF THREE CRYSTAL FORMS OF THE SWEET PROTEIN THAUMATIN==
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Three crystal forms of the sweet-tasting protein thaumatin from the, African berry Thaumatococcus daniellii have been grown. These include two, naturally occurring isoforms, A and B, that differ by a single amino acid, and a recombinant form of isoform B expressed in yeast. The crystals are, of space groups C2 with a = 117.7, b = 44.9, c = 38.0 A, and beta = 94.0, degrees, P2(1)2(1)2(1) with a = 44.3, b = 63.7 and c = 72.7 A, and a, tetragonal form P4(1)2(1)2 with a = b = 58.6 and c = 151.8 A. The, structures of all three crystals have been solved by molecular replacement, and subsequently refined to R factors of 0.184 for the monoclinic at 2.6, A, 0.165 for the orthorhombic at 1.75 A, and 0.181 for the tetragonal, also at 1.75 A resolution. No solvent was included in the monoclinic, crystal while 123 and 105 water molecules were included in the higher, resolution orthorhombic and tetragonal structures, respectively. A bound, tartrate molecule was also clearly visible in the tetragonal structure., The r.m.s. deviations between molecular structures in the three crystals, range from 0.6 to 0.7 A for Calpha atoms, and 1.1 to 1.3 A for all atoms., This is comparable to the r.m.s. deviation between the three structures, and the starting model. Nevertheless, several peptide loops show, particularly large variations from the initial model.
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<StructureSection load='1thu' size='340' side='right'caption='[[1thu]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1thu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thaumatococcus_daniellii Thaumatococcus daniellii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1THU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1THU FirstGlance]. <br>
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1THU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thaumatococcus_daniellii Thaumatococcus daniellii]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1THU OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1thu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1thu OCA], [https://pdbe.org/1thu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1thu RCSB], [https://www.ebi.ac.uk/pdbsum/1thu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1thu ProSAT]</span></td></tr>
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==Reference==
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</table>
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Structures of three crystal forms of the sweet protein thaumatin., Ko TP, Day J, Greenwood A, McPherson A, Acta Crystallogr D Biol Crystallogr. 1994 Nov 1;50(Pt 6):813-25. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15299348 15299348]
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== Function ==
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[[Category: Single protein]]
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[https://www.uniprot.org/uniprot/THM1_THADA THM1_THADA] Taste-modifying protein; intensely sweet-tasting. It is 100000 times sweeter than sucrose on a molar basis.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/th/1thu_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1thu ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Thaumatococcus daniellii]]
[[Category: Thaumatococcus daniellii]]
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[[Category: Day, J.]]
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[[Category: Day J]]
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[[Category: Greenwood, A.]]
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[[Category: Greenwood A]]
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[[Category: Ko, T.P.]]
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[[Category: Ko T-P]]
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[[Category: McPherson, A.]]
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[[Category: McPherson A]]
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[[Category: sweet tasting protein]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 03:16:38 2007''
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THE STRUCTURES OF THREE CRYSTAL FORMS OF THE SWEET PROTEIN THAUMATIN

PDB ID 1thu

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