1tj7

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(New page: 200px<br /><applet load="1tj7" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tj7, resolution 2.44&Aring;" /> '''Structure determinat...)
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[[Image:1tj7.gif|left|200px]]<br /><applet load="1tj7" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1tj7, resolution 2.44&Aring;" />
 
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'''Structure determination and refinement at 2.44 A resolution of Argininosuccinate lyase from E. coli'''<br />
 
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==Overview==
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==Structure determination and refinement at 2.44 A resolution of Argininosuccinate lyase from E. coli==
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Escherichia coli argininosuccinate lyase has been crystallized from a, highly concentrated sample of purified recombinant alpha-methylacyl-CoA, racemase, in which it occurred as a minor impurity. The structure has been, solved using molecular replacement at 2.44 A resolution. The enzyme is, tetrameric, but in this crystal form there is a dimer in the asymmetric, unit. The tetramer has four active sites; each active site is constructed, from loops of three different subunits. One of these catalytic loops, near, residues Ser277 and Ser278, was disordered in previous structures of, active lyases, but is very well ordered in this structure in one of the, subunits owing to the presence of two phosphate ions in the active-site, cavity. The positions of these phosphate ions indicate a plausible mode of, binding of the succinate moiety of the substrate in the competent, catalytic complex.
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<StructureSection load='1tj7' size='340' side='right'caption='[[1tj7]], [[Resolution|resolution]] 2.44&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1tj7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TJ7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TJ7 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.44&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tj7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tj7 OCA], [https://pdbe.org/1tj7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tj7 RCSB], [https://www.ebi.ac.uk/pdbsum/1tj7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tj7 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ARLY_ECOLI ARLY_ECOLI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tj/1tj7_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tj7 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Escherichia coli argininosuccinate lyase has been crystallized from a highly concentrated sample of purified recombinant alpha-methylacyl-CoA racemase, in which it occurred as a minor impurity. The structure has been solved using molecular replacement at 2.44 A resolution. The enzyme is tetrameric, but in this crystal form there is a dimer in the asymmetric unit. The tetramer has four active sites; each active site is constructed from loops of three different subunits. One of these catalytic loops, near residues Ser277 and Ser278, was disordered in previous structures of active lyases, but is very well ordered in this structure in one of the subunits owing to the presence of two phosphate ions in the active-site cavity. The positions of these phosphate ions indicate a plausible mode of binding of the succinate moiety of the substrate in the competent catalytic complex.
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==About this Structure==
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Structure determination and refinement at 2.44 A resolution of argininosuccinate lyase from Escherichia coli.,Bhaumik P, Koski MK, Bergmann U, Wierenga RK Acta Crystallogr D Biol Crystallogr. 2004 Nov;60(Pt 11):1964-70. Epub 2004, Oct 20. PMID:15502303<ref>PMID:15502303</ref>
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1TJ7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with PO4 and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Argininosuccinate_lyase Argininosuccinate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.2.1 4.3.2.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1TJ7 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structure determination and refinement at 2.44 A resolution of argininosuccinate lyase from Escherichia coli., Bhaumik P, Koski MK, Bergmann U, Wierenga RK, Acta Crystallogr D Biol Crystallogr. 2004 Nov;60(Pt 11):1964-70. Epub 2004, Oct 20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15502303 15502303]
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</div>
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[[Category: Argininosuccinate lyase]]
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<div class="pdbe-citations 1tj7" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Bergman, U.]]
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[[Category: Bergman U]]
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[[Category: Bhaumik, P.]]
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[[Category: Bhaumik P]]
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[[Category: Koski, M.K.]]
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[[Category: Koski MK]]
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[[Category: Wierenga, R.K.]]
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[[Category: Wierenga RK]]
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[[Category: GOL]]
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[[Category: PO4]]
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[[Category: argininosuccinate lyase]]
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[[Category: aspartase]]
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[[Category: crystallin]]
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[[Category: e. coli]]
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[[Category: fumarase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 03:18:58 2007''
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Current revision

Structure determination and refinement at 2.44 A resolution of Argininosuccinate lyase from E. coli

PDB ID 1tj7

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