1tks

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(New page: 200px<br /><applet load="1tks" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tks, resolution 1.6&Aring;" /> '''Crystal structure of ...)
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[[Image:1tks.gif|left|200px]]<br /><applet load="1tks" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1tks, resolution 1.6&Aring;" />
 
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'''Crystal structure of 3,4-Dihydroxy-2-butanone 4-phosphate Synthase of Candida albicans'''<br />
 
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==Overview==
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==Crystal structure of 3,4-Dihydroxy-2-butanone 4-phosphate Synthase of Candida albicans==
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A synthetic gene specifying a putative 3,4-dihydroxy-2-butanone, 4-phosphate synthase of Candida albicans directed the synthesis of a 22.5, kDa peptide in a recombinant Escherichia coli strain. The recombinant, protein was purified to apparent homogeneity by two chromatographic steps, and was shown to catalyze the formation of L-3,4-dihydroxy-2-butanone, 4-phosphate from ribulose 5-phosphate at a rate of 332 nmol mg(-1), min(-1). Hydrodynamic studies indicated a native molecular mass of 41 kDa, in line with a homodimer structure. The protein was crystallized in its, apoform. Soaking yielded crystals in complex with the substrate ribulose, 5-phosphate. The structures were solved at resolutions of 1.6 and 1.7, angstroms, respectively, using 3,4-dihydroxy-2-butanone 4-phosphate, synthase of E. coli for molecular replacement. Structural comparison with, the orthologs of Magnaporthe grisea and Methanococcus jannaschii revealed, a hitherto unknown conformation of the essential acidic active-site loop.
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<StructureSection load='1tks' size='340' side='right'caption='[[1tks]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1tks]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Candida_albicans_SC5314 Candida albicans SC5314]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TKS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TKS FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tks OCA], [https://pdbe.org/1tks PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tks RCSB], [https://www.ebi.ac.uk/pdbsum/1tks PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tks ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RIB3_CANAL RIB3_CANAL] Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate (By similarity).[HAMAP-Rule:MF_00180]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tk/1tks_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tks ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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A synthetic gene specifying a putative 3,4-dihydroxy-2-butanone 4-phosphate synthase of Candida albicans directed the synthesis of a 22.5 kDa peptide in a recombinant Escherichia coli strain. The recombinant protein was purified to apparent homogeneity by two chromatographic steps and was shown to catalyze the formation of L-3,4-dihydroxy-2-butanone 4-phosphate from ribulose 5-phosphate at a rate of 332 nmol mg(-1) min(-1). Hydrodynamic studies indicated a native molecular mass of 41 kDa in line with a homodimer structure. The protein was crystallized in its apoform. Soaking yielded crystals in complex with the substrate ribulose 5-phosphate. The structures were solved at resolutions of 1.6 and 1.7 angstroms, respectively, using 3,4-dihydroxy-2-butanone 4-phosphate synthase of E. coli for molecular replacement. Structural comparison with the orthologs of Magnaporthe grisea and Methanococcus jannaschii revealed a hitherto unknown conformation of the essential acidic active-site loop.
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==About this Structure==
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Potential anti-infective targets in pathogenic yeasts: structure and properties of 3,4-dihydroxy-2-butanone 4-phosphate synthase of Candida albicans.,Echt S, Bauer S, Steinbacher S, Huber R, Bacher A, Fischer M J Mol Biol. 2004 Aug 20;341(4):1085-96. PMID:15328619<ref>PMID:15328619</ref>
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1TKS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Candida_albicans_sc5314 Candida albicans sc5314]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1TKS OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Potential anti-infective targets in pathogenic yeasts: structure and properties of 3,4-dihydroxy-2-butanone 4-phosphate synthase of Candida albicans., Echt S, Bauer S, Steinbacher S, Huber R, Bacher A, Fischer M, J Mol Biol. 2004 Aug 20;341(4):1085-96. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15328619 15328619]
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</div>
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[[Category: Candida albicans sc5314]]
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<div class="pdbe-citations 1tks" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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== References ==
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[[Category: Bacher, A.]]
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<references/>
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[[Category: Bauer, S.]]
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__TOC__
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[[Category: Echt, S.]]
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</StructureSection>
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[[Category: Fischer, M.]]
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[[Category: Candida albicans SC5314]]
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[[Category: Huber, R.]]
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[[Category: Large Structures]]
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[[Category: Steinbacher, S.]]
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[[Category: Bacher A]]
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[[Category: 3]]
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[[Category: Bauer S]]
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[[Category: 4-dihydroxy-2-butanone 4-phosphate synthase]]
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[[Category: Echt S]]
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[[Category: candida albicans]]
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[[Category: Fischer M]]
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[[Category: crystal structure synthetic gene]]
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[[Category: Huber R]]
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[[Category: riboflavin biosynthesis]]
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[[Category: Steinbacher S]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 03:21:40 2007''
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Current revision

Crystal structure of 3,4-Dihydroxy-2-butanone 4-phosphate Synthase of Candida albicans

PDB ID 1tks

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