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1tkw

From Proteopedia

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(New page: 200px<br /><applet load="1tkw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tkw" /> '''The transient complex of poplar plastocyanin...)
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[[Image:1tkw.gif|left|200px]]<br /><applet load="1tkw" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1tkw" />
 
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'''The transient complex of poplar plastocyanin with turnip cytochrome f determined with paramagnetic NMR'''<br />
 
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==Overview==
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==The transient complex of poplar plastocyanin with turnip cytochrome f determined with paramagnetic NMR==
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The orientation of poplar plastocyanin in the complex with turnip, cytochrome f has been determined by rigid-body calculations using, restraints from paramagnetic NMR measurements. The results show that, poplar plastocyanin interacts with cytochrome f with the hydrophobic patch, of plastocyanin close to the heme region on cytochrome f and via, electrostatic interactions between the charged patches on both proteins., Plastocyanin is tilted relative to the orientation reported for spinach, plastocyanin, resulting in a longer distance between iron and copper (13.9, A). With increasing ionic strength, from 0.01 to 0.11 M, all observed, chemical-shift changes decrease uniformly, supporting the idea that, electrostatic forces contribute to complex formation. There is no, indication for a rearrangement of the transient complex in this ionic, strength range, contrary to what had been proposed earlier on the basis of, kinetic data. By decreasing the pH from pH 7.7 to pH 5.5, the complex is, destabilized. This may be attributed to the protonation of the conserved, acidic patches or the copper ligand His87 in poplar plastocyanin, which, are shown to have similar pK(a) values. The results are interpreted in a, two-step model for complex formation.
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<StructureSection load='1tkw' size='340' side='right'caption='[[1tkw]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1tkw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Brassica_rapa_subsp._rapa Brassica rapa subsp. rapa] and [https://en.wikipedia.org/wiki/Populus_nigra Populus nigra]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TKW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TKW FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tkw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tkw OCA], [https://pdbe.org/1tkw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tkw RCSB], [https://www.ebi.ac.uk/pdbsum/1tkw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tkw ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PLAS1_POPNI PLAS1_POPNI] Participates in electron transfer between P700 and the cytochrome b6-f complex in photosystem I.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tk/1tkw_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tkw ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1TKW is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Brassica_rapa Brassica rapa] and [http://en.wikipedia.org/wiki/Populus_nigra Populus nigra] with CU and HEC as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1TKW OCA].
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*[[Cytochrome f 3D structures|Cytochrome f 3D structures]]
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*[[Plastocyanin 3D structures|Plastocyanin 3D structures]]
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==Reference==
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__TOC__
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The transient complex of poplar plastocyanin with cytochrome f: effects of ionic strength and pH., Lange C, Cornvik T, Diaz-Moreno I, Ubbink M, Biochim Biophys Acta. 2005 Apr-May;1707(2-3):179-88. Epub 2004 Dec 29. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15863096 15863096]
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</StructureSection>
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[[Category: Brassica rapa]]
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[[Category: Brassica rapa subsp. rapa]]
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[[Category: Large Structures]]
[[Category: Populus nigra]]
[[Category: Populus nigra]]
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[[Category: Protein complex]]
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[[Category: Cornvik T]]
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[[Category: Cornvik, T.]]
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[[Category: Diaz-Moreno I]]
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[[Category: Diaz-Moreno, I.]]
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[[Category: Lange C]]
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[[Category: Lange, C.]]
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[[Category: Ubbink M]]
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[[Category: Ubbink, M.]]
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[[Category: CU]]
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[[Category: HEC]]
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[[Category: electron transfer]]
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[[Category: nmr]]
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[[Category: paramagnetic]]
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[[Category: photosynthesis]]
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[[Category: rigid body calculations]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 03:21:52 2007''
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Current revision

The transient complex of poplar plastocyanin with turnip cytochrome f determined with paramagnetic NMR

PDB ID 1tkw

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