1tll

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(New page: 200px<br /><applet load="1tll" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tll, resolution 2.30&Aring;" /> '''CRYSTAL STRUCTURE OF...)
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[[Image:1tll.gif|left|200px]]<br /><applet load="1tll" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1tll, resolution 2.30&Aring;" />
 
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'''CRYSTAL STRUCTURE OF RAT NEURONAL NITRIC-OXIDE SYNTHASE REDUCTASE MODULE AT 2.3 A RESOLUTION.'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF RAT NEURONAL NITRIC-OXIDE SYNTHASE REDUCTASE MODULE AT 2.3 A RESOLUTION.==
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Three nitric-oxide synthase (NOS) isozymes play crucial, but distinct, roles in neurotransmission, vascular homeostasis, and host defense, by, catalyzing Ca(2+)/calmodulin-triggered NO synthesis. Here, we address, current questions regarding NOS activity and regulation by combining, mutagenesis and biochemistry with crystal structure determination of a, fully assembled, electron-supplying, neuronal NOS reductase dimer. By, integrating these results, we structurally elucidate the unique mechanisms, for isozyme-specific regulation of electron transfer in NOS. Our discovery, of the autoinhibitory helix, its placement between domains, and striking, similarities with canonical calmodulin-binding motifs, support new, mechanisms for NOS inhibition. NADPH, isozyme-specific residue Arg(1400), and the C-terminal tail synergistically repress NOS activity by locking, the FMN binding domain in an electron-accepting position. Our analyses, suggest that calmodulin binding or C-terminal tail phosphorylation frees a, large scale swinging motion of the entire FMN domain to deliver electrons, to the catalytic module in the holoenzyme.
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<StructureSection load='1tll' size='340' side='right'caption='[[1tll]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1tll]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. The January 2011 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Nitric Oxide Synthase'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2011_1 10.2210/rcsb_pdb/mom_2011_1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TLL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TLL FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=SO3:SULFITE+ION'>SO3</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tll FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tll OCA], [https://pdbe.org/1tll PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tll RCSB], [https://www.ebi.ac.uk/pdbsum/1tll PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tll ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NOS1_RAT NOS1_RAT] Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Inhibitory transmitter for non-adrenergic and non-cholinergic nerves in the colorectum. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR. Inhibitory transmitter for non-adrenergic and non-cholinergic nerves in the colorectum.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tl/1tll_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tll ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Three nitric-oxide synthase (NOS) isozymes play crucial, but distinct, roles in neurotransmission, vascular homeostasis, and host defense, by catalyzing Ca(2+)/calmodulin-triggered NO synthesis. Here, we address current questions regarding NOS activity and regulation by combining mutagenesis and biochemistry with crystal structure determination of a fully assembled, electron-supplying, neuronal NOS reductase dimer. By integrating these results, we structurally elucidate the unique mechanisms for isozyme-specific regulation of electron transfer in NOS. Our discovery of the autoinhibitory helix, its placement between domains, and striking similarities with canonical calmodulin-binding motifs, support new mechanisms for NOS inhibition. NADPH, isozyme-specific residue Arg(1400), and the C-terminal tail synergistically repress NOS activity by locking the FMN binding domain in an electron-accepting position. Our analyses suggest that calmodulin binding or C-terminal tail phosphorylation frees a large scale swinging motion of the entire FMN domain to deliver electrons to the catalytic module in the holoenzyme.
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==About this Structure==
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Structural basis for isozyme-specific regulation of electron transfer in nitric-oxide synthase.,Garcin ED, Bruns CM, Lloyd SJ, Hosfield DJ, Tiso M, Gachhui R, Stuehr DJ, Tainer JA, Getzoff ED J Biol Chem. 2004 Sep 3;279(36):37918-27. Epub 2004 Jun 17. PMID:15208315<ref>PMID:15208315</ref>
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1TLL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with SO3, FMN, FAD and NAP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Nitric-oxide_synthase Nitric-oxide synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.13.39 1.14.13.39] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1TLL OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structural basis for isozyme-specific regulation of electron transfer in nitric-oxide synthase., Garcin ED, Bruns CM, Lloyd SJ, Hosfield DJ, Tiso M, Gachhui R, Stuehr DJ, Tainer JA, Getzoff ED, J Biol Chem. 2004 Sep 3;279(36):37918-27. Epub 2004 Jun 17. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15208315 15208315]
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</div>
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[[Category: Nitric-oxide synthase]]
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<div class="pdbe-citations 1tll" style="background-color:#fffaf0;"></div>
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[[Category: Rattus norvegicus]]
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[[Category: Single protein]]
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[[Category: Bruns, C.M.]]
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[[Category: Gachhui, R.]]
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[[Category: Garcin, E.D.]]
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[[Category: Getzoff, E.D.]]
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[[Category: Hosfield, D.J.]]
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[[Category: Lloyd, S.J.]]
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[[Category: Stuehr, D.J.]]
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[[Category: Tainer, J.A.]]
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[[Category: Tiso, M.]]
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[[Category: FAD]]
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[[Category: FMN]]
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[[Category: NAP]]
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[[Category: SO3]]
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[[Category: fad]]
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[[Category: fmn]]
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[[Category: nadp+]]
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[[Category: nitric-oxide synthase]]
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[[Category: reductase module]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 03:23:04 2007''
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==See Also==
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*[[Nitric Oxide Synthase|Nitric Oxide Synthase]]
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*[[Nitric Oxide Synthase 3D structures|Nitric Oxide Synthase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Nitric Oxide Synthase]]
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[[Category: RCSB PDB Molecule of the Month]]
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[[Category: Rattus norvegicus]]
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[[Category: Bruns CM]]
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[[Category: Gachhui R]]
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[[Category: Garcin ED]]
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[[Category: Getzoff ED]]
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[[Category: Hosfield DJ]]
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[[Category: Lloyd SJ]]
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[[Category: Stuehr DJ]]
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[[Category: Tainer JA]]
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[[Category: Tiso M]]

Current revision

CRYSTAL STRUCTURE OF RAT NEURONAL NITRIC-OXIDE SYNTHASE REDUCTASE MODULE AT 2.3 A RESOLUTION.

PDB ID 1tll

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