2zn8

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{{Seed}}
 
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[[Image:2zn8.png|left|200px]]
 
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==Crystal structure of Zn2+-bound form of ALG-2==
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The line below this paragraph, containing "STRUCTURE_2zn8", creates the "Structure Box" on the page.
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<StructureSection load='2zn8' size='340' side='right'caption='[[2zn8]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2zn8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZN8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZN8 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_2zn8| PDB=2zn8 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zn8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zn8 OCA], [https://pdbe.org/2zn8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zn8 RCSB], [https://www.ebi.ac.uk/pdbsum/2zn8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zn8 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PDCD6_HUMAN PDCD6_HUMAN] Calcium-binding protein required for T-cell receptor-, Fas-, and glucocorticoid-induced cell death. May mediate Ca(2+)-regulated signals along the death pathway (By similarity). Calcium-dependent adapter necessary for the association between PDCD6IP and TSG101. Interaction with DAPK1 can accelerate apoptotic cell death by increasing caspase-3 activity.<ref>PMID:16132846</ref> <ref>PMID:19520058</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zn/2zn8_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2zn8 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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ALG-2 belongs to the penta-EF-hand (PEF) protein family and interacts with various intracellular proteins, such as Alix and TSG101, that are involved in endosomal sorting and HIV budding. Through X-ray crystallography, we solved the structures of Ca(2+)-free and -bound forms of N-terminally truncated human ALG-2 (des3-20ALG-2), Zn(2+)-bound form of full-length ALG-2, and the structure of the complex between des3-23ALG-2 and the peptide corresponding to Alix799-814 in Zn(2+)-bound form. Binding of Ca(2+) to EF3 enables the side chain of Arg125, present in the loop connecting EF3 and EF4, to move enough to make a primary hydrophobic pocket accessible to the critical PPYP motif, which partially overlaps with the GPP motif for the binding of Cep55 (centrosome protein 55 kDa). Based on these results, together with the results of in vitro binding assay with mutant ALG-2 and Alix proteins, we propose a Ca(2+)/EF3-driven arginine switch mechanism for ALG-2 binding to Alix.
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===Crystal structure of Zn2+-bound form of ALG-2===
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Structural basis for Ca2+ -dependent formation of ALG-2/Alix peptide complex: Ca2+/EF3-driven arginine switch mechanism.,Suzuki H, Kawasaki M, Inuzuka T, Okumura M, Kakiuchi T, Shibata H, Wakatsuki S, Maki M Structure. 2008 Oct 8;16(10):1562-73. PMID:18940611<ref>PMID:18940611</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2zn8" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_18940611}}, adds the Publication Abstract to the page
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*[[Cell death protein 3D structures|Cell death protein 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 18940611 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_18940611}}
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__TOC__
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</StructureSection>
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==About this Structure==
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2ZN8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZN8 OCA].
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==Reference==
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Structural basis for Ca2+ -dependent formation of ALG-2/Alix peptide complex: Ca2+/EF3-driven arginine switch mechanism., Suzuki H, Kawasaki M, Inuzuka T, Okumura M, Kakiuchi T, Shibata H, Wakatsuki S, Maki M, Structure. 2008 Oct 8;16(10):1562-73. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18940611 18940611]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Inuzuka, T.]]
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[[Category: Inuzuka T]]
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[[Category: Kakiuchi, T.]]
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[[Category: Kakiuchi T]]
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[[Category: Kawasaki, M.]]
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[[Category: Kawasaki M]]
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[[Category: Maki, M.]]
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[[Category: Maki M]]
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[[Category: Shibata, H.]]
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[[Category: Shibata H]]
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[[Category: Suzuki, H.]]
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[[Category: Suzuki H]]
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[[Category: Wakatsuki, S.]]
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[[Category: Wakatsuki S]]
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[[Category: Apoptosis]]
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[[Category: Calcium]]
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[[Category: Calcium binding protein]]
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[[Category: Endoplasmic reticulum]]
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[[Category: Membrane]]
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[[Category: Nucleus]]
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[[Category: Penta-ef-hand protein]]
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[[Category: Polymorphism]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Nov 5 10:38:22 2008''
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Current revision

Crystal structure of Zn2+-bound form of ALG-2

PDB ID 2zn8

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