3e3j

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{{Seed}}
 
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[[Image:3e3j.jpg|left|200px]]
 
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==Crystal Structure of an Intermediate Complex of T7 RNAP and 8nt of RNA==
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The line below this paragraph, containing "STRUCTURE_3e3j", creates the "Structure Box" on the page.
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<StructureSection load='3e3j' size='340' side='right'caption='[[3e3j]], [[Resolution|resolution]] 6.70&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3e3j]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_phage_T7 Escherichia phage T7] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E3J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3E3J FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 6.7&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3e3j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3e3j OCA], [https://pdbe.org/3e3j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3e3j RCSB], [https://www.ebi.ac.uk/pdbsum/3e3j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3e3j ProSAT]</span></td></tr>
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{{STRUCTURE_3e3j| PDB=3e3j | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RPOL_BPT7 RPOL_BPT7] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Responsible for the transcription of the late genes of T7. It is rifampicin-resistant. It recognizes a specific promoter sequence, unwinds the double-stranded RNA to expose the coding strand for templating, initiates transcription preferentially with a purine.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e3/3e3j_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3e3j ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Structural studies of the T7 bacteriophage DNA-dependent RNA polymerase (T7 RNAP) have shown that the conformation of the amino-terminal domain changes substantially between the initiation and elongation phases of transcription, but how this transition is achieved remains unclear. We report crystal structures of T7 RNAP bound to promoter DNA containing either a 7- or an 8-nucleotide (nt) RNA transcript that illuminate intermediate states along the transition pathway. The amino-terminal domain comprises the C-helix subdomain and the promoter binding domain (PBD), which consists of two segments separated by subdomain H. The structures of the intermediate complex reveal that the PBD and the bound promoter rotate by approximately 45 degrees upon synthesis of an 8-nt RNA transcript. This allows the promoter contacts to be maintained while the active site is expanded to accommodate a growing heteroduplex. The C-helix subdomain moves modestly toward its elongation conformation, whereas subdomain H remains in its initiation- rather than its elongation-phase location, more than 70 angstroms away.
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===Crystal Structure of an Intermediate Complex of T7 RNAP and 8nt of RNA===
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The structure of a transcribing t7 RNA polymerase in transition from initiation to elongation.,Durniak KJ, Bailey S, Steitz TA Science. 2008 Oct 24;322(5901):553-7. PMID:18948533<ref>PMID:18948533</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3e3j" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_18948533}}, adds the Publication Abstract to the page
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*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 18948533 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_18948533}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Escherichia phage T7]]
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3E3J is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t7 Enterobacteria phage t7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E3J OCA].
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[[Category: Large Structures]]
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[[Category: Synthetic construct]]
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==Reference==
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[[Category: Bailey S]]
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The structure of a transcribing t7 RNA polymerase in transition from initiation to elongation., Durniak KJ, Bailey S, Steitz TA, Science. 2008 Oct 24;322(5901):553-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18948533 18948533]
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[[Category: Durniak KJ]]
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[[Category: DNA-directed RNA polymerase]]
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[[Category: Steitz TA]]
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[[Category: Enterobacteria phage t7]]
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[[Category: Protein complex]]
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[[Category: Bailey, S.]]
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[[Category: Durniak, K J.]]
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[[Category: Steitz, T A.]]
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[[Category: Dna-directed rna polymerase]]
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[[Category: Nucleotidyltransferase]]
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[[Category: T7 rna polymerase]]
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[[Category: Transcription]]
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[[Category: Transferase]]
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[[Category: Transferase/rna/dna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Nov 5 12:51:53 2008''
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Current revision

Crystal Structure of an Intermediate Complex of T7 RNAP and 8nt of RNA

PDB ID 3e3j

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