This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1tuk

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1tuk" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tuk, resolution 1.12&Aring;" /> '''Crystal stucture of ...)
Current revision (08:44, 1 May 2024) (edit) (undo)
 
(17 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1tuk.gif|left|200px]]<br /><applet load="1tuk" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1tuk, resolution 1.12&Aring;" />
 
-
'''Crystal stucture of liganted type 2 non specific lipid transfer protein from wheat'''<br />
 
-
==Overview==
+
==Crystal structure of liganded type 2 non specific lipid transfer protein from wheat==
-
In plants, a family of ubiquitous proteins named non-specific, lipid-transfer proteins (ns-LTPs) facilitates the transfer of fatty acids, phospholipids and steroids between membranes. Recent data suggest that, these secreted proteins play a key role in the formation of cuticular wax, layers and in defence mechanisms against pathogens. In this study, X-ray, crystallography has been used to examine the structural details of the, interaction between a wheat type 2 ns-LTP and a lipid, L-alpha-palmitoyl-phosphatidyl glycerol. This crystal structure was solved, ab initio at 1.12 A resolution by direct methods. The typical, alpha-helical bundle fold of this protein is maintained by four disulfide, bridges and delineates two hydrophobic cavities. The inner surface of the, main cavity is lined by non-polar residues that provide a hydrophobic, environment for the palmitoyl moiety of the lipid. The head-group region, of this lipid protrudes from the surface and makes several polar, interactions with a conserved patch of basic residues at the entrance of, the pocket. The alkyl chain of a second lipid is bound within an adjacent, smaller cavity. The structure shows that binding of the lipid tails to the, protein involves extensive hydrophobic interactions.
+
<StructureSection load='1tuk' size='340' side='right'caption='[[1tuk]], [[Resolution|resolution]] 1.12&Aring;' scene=''>
-
 
+
== Structural highlights ==
-
==About this Structure==
+
<table><tr><td colspan='2'>[[1tuk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Triticum_aestivum Triticum aestivum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TUK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TUK FirstGlance]. <br>
-
1TUK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Triticum_aestivum Triticum aestivum] with IOD and PGM as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1TUK OCA].
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.12&#8491;</td></tr>
-
 
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=PGM:1-MYRISTOYL-2-HYDROXY-SN-GLYCERO-3-[PHOSPHO-RAC-(1-GLYCEROL)]'>PGM</scene></td></tr>
-
==Reference==
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tuk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tuk OCA], [https://pdbe.org/1tuk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tuk RCSB], [https://www.ebi.ac.uk/pdbsum/1tuk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tuk ProSAT]</span></td></tr>
-
Structure of a liganded type 2 non-specific lipid-transfer protein from wheat and the molecular basis of lipid binding., Hoh F, Pons JL, Gautier MF, de Lamotte F, Dumas C, Acta Crystallogr D Biol Crystallogr. 2005 Apr;61(Pt 4):397-406. Epub 2005, Mar 24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15805594 15805594]
+
</table>
-
[[Category: Single protein]]
+
== Function ==
 +
[https://www.uniprot.org/uniprot/NLT2G_WHEAT NLT2G_WHEAT] Transfer lipids across membranes. May play a role in plant defense or in the biosynthesis of cuticle layers.<ref>PMID:11231292</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tu/1tuk_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tuk ConSurf].
 +
<div style="clear:both"></div>
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Large Structures]]
[[Category: Triticum aestivum]]
[[Category: Triticum aestivum]]
-
[[Category: Dumas, C.]]
+
[[Category: De Lamotte F]]
-
[[Category: Gautier, M.F.]]
+
[[Category: Dumas C]]
-
[[Category: Hoh, F.]]
+
[[Category: Gautier MF]]
-
[[Category: Lamotte, F.De.]]
+
[[Category: Hoh F]]
-
[[Category: Pons, J.L.]]
+
[[Category: Pons JL]]
-
[[Category: IOD]]
+
-
[[Category: PGM]]
+
-
[[Category: lipid transfer protein]]
+
-
[[Category: ns-ltp2]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 03:36:12 2007''
+

Current revision

Crystal structure of liganded type 2 non specific lipid transfer protein from wheat

PDB ID 1tuk

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1tuk"
Personal tools