3dhv

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (12:47, 30 August 2023) (edit) (undo)
 
(9 intermediate revisions not shown.)
Line 1: Line 1:
-
{{Seed}}
 
-
[[Image:3dhv.png|left|200px]]
 
-
<!--
+
==Crystal structure of DltA protein in complex with D-alanine adenylate==
-
The line below this paragraph, containing "STRUCTURE_3dhv", creates the "Structure Box" on the page.
+
<StructureSection load='3dhv' size='340' side='right'caption='[[3dhv]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[3dhv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_cereus_ATCC_14579 Bacillus cereus ATCC 14579]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DHV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3DHV FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=DAL:D-ALANINE'>DAL</scene></td></tr>
-
{{STRUCTURE_3dhv| PDB=3dhv | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3dhv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dhv OCA], [https://pdbe.org/3dhv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3dhv RCSB], [https://www.ebi.ac.uk/pdbsum/3dhv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3dhv ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/DLTA_BACCR DLTA_BACCR] Involved in the biosynthesis of D-alanyl-lipoteichoic acid (LTA). Catalyzes an ATP-dependent two-step reaction where it forms a high energy D-alanyl AMP intermediate and transfers the alanyl residues from AMP to Dcp (By similarity).
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dh/3dhv_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3dhv ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Ubiquitous D-alanylation of lipoteichoic acids modulates the surface charge and ligand binding of the gram-positive cell wall. Disruption of the bacterial DltABCD gene involved in teichoic acid alanylation, as well as inhibition of the DltA protein, has been shown to increase a gram-positive bacterium's susceptibility to antibiotics. The DltA D-alanyl carrier protein ligase promotes a two-step process starting with adenylation of D-alanine. We have determined the 2.0 A resolution crystal structure of a DltA protein from Bacillus cereus in complex with the D-alanine adenylate intermediate of the first reaction. Despite the low level of sequence similarity, the DltA structure resembles known structures of adenylation domains such as the acetyl-CoA synthetase. The enantiomer selection appears to be enhanced by the medium-sized side chain of Cys-269. The Ala-269 mutant protein shows marked loss of such selection. The network of noncovalent interactions between the D-alanine adenylate and DltA provides structure-based rationale for aiding the design of tight-binding DltA inhibitors for combating infectious gram-positive bacteria such as the notorious methicillin-resistant Staphylococcus aureus.
-
===Crystal structure of DltA protein in complex with D-alanine adenylate===
+
Crystal structure and enantiomer selection by D-alanyl carrier protein ligase DltA from Bacillus cereus.,Du L, He Y, Luo Y Biochemistry. 2008 Nov 4;47(44):11473-80. Epub 2008 Oct 11. PMID:18847223<ref>PMID:18847223</ref>
-
 
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
<!--
+
</div>
-
The line below this paragraph, {{ABSTRACT_PUBMED_18847223}}, adds the Publication Abstract to the page
+
<div class="pdbe-citations 3dhv" style="background-color:#fffaf0;"></div>
-
(as it appears on PubMed at http://www.pubmed.gov), where 18847223 is the PubMed ID number.
+
== References ==
-
-->
+
<references/>
-
{{ABSTRACT_PUBMED_18847223}}
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
[[Category: Bacillus cereus ATCC 14579]]
-
3DHV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_cereus Bacillus cereus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DHV OCA].
+
[[Category: Large Structures]]
-
 
+
[[Category: Du L]]
-
==Reference==
+
[[Category: He Y]]
-
Crystal structure and enantiomer selection by D-alanyl carrier protein ligase DltA from Bacillus cereus., Du L, He Y, Luo Y, Biochemistry. 2008 Nov 4;47(44):11473-80. Epub 2008 Oct 11. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18847223 18847223]
+
[[Category: Luo Y]]
-
[[Category: Bacillus cereus]]
+
-
[[Category: Single protein]]
+
-
[[Category: Du, L.]]
+
-
[[Category: He, Y.]]
+
-
[[Category: Luo, Y.]]
+
-
[[Category: Adenylation]]
+
-
[[Category: Amp-forming domain]]
+
-
[[Category: Cytoplasm]]
+
-
[[Category: D-alanine]]
+
-
[[Category: D-alanine carrier protein ligase]]
+
-
[[Category: Dlta]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Nov 12 20:30:40 2008''
+

Current revision

Crystal structure of DltA protein in complex with D-alanine adenylate

PDB ID 3dhv

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3dhv"
Personal tools