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1tv4

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(New page: 200px<br /><applet load="1tv4" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tv4, resolution 1.80&Aring;" /> '''Crystal structure of...)
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[[Image:1tv4.jpg|left|200px]]<br /><applet load="1tv4" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1tv4, resolution 1.80&Aring;" />
 
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'''Crystal structure of the sulfite MtmB complex'''<br />
 
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==Overview==
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==Crystal structure of the sulfite MtmB complex==
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L-pyrrolysine, the 22(nd) genetically encoded amino acid, was previously, deduced to be (4R, 5R)-4-substituted-pyrroline-5-carboxylate attached to, the epsilon-nitrogen of lysine based on the crystal structure of the M., barkeri monomethylamine methyltransferase (MtmB). To confirm, L-pyrrolysine's identity, structures of MtmB have been determined, following treatment with hydroxylamine, N-methylhydroxylamine, or, dithionite. Analysis of these structures has provided additional support, for the presence of the pyrroline ring and, together with previous mass, spectroscopy data, has led us to assign the C(4)-substituent to a methyl, group. Based on this assignment, synthetic L-pyrrolysine was prepared by, chemical methods. Detailed study of this chemically synthesized, L-pyrrolysine has allowed us to characterize its physical properties, to, study its chemical stability, and to elucidate the role of its C(4), substituent. Future applications of this synthetic L-pyrrolysine include, its in vivo incorporation into recombinant proteins.
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<StructureSection load='1tv4' size='340' side='right'caption='[[1tv4]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1tv4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanosarcina_barkeri Methanosarcina barkeri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TV4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TV4 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BG3:3-METHYL-5-SULFO-PYRROLIDINE-2-CARBOXYLIC+ACID'>BG3</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tv4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tv4 OCA], [https://pdbe.org/1tv4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tv4 RCSB], [https://www.ebi.ac.uk/pdbsum/1tv4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tv4 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MTMB1_METBA MTMB1_METBA] Catalyzes the transfer of the methyl group from monomethylamine to the corrinoid cofactor of MtmC (MtmC1 or MtmC2).<ref>PMID:9642198</ref> <ref>PMID:9195968</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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L-pyrrolysine, the 22(nd) genetically encoded amino acid, was previously deduced to be (4R, 5R)-4-substituted-pyrroline-5-carboxylate attached to the epsilon-nitrogen of lysine based on the crystal structure of the M. barkeri monomethylamine methyltransferase (MtmB). To confirm L-pyrrolysine's identity, structures of MtmB have been determined following treatment with hydroxylamine, N-methylhydroxylamine, or dithionite. Analysis of these structures has provided additional support for the presence of the pyrroline ring and, together with previous mass spectroscopy data, has led us to assign the C(4)-substituent to a methyl group. Based on this assignment, synthetic L-pyrrolysine was prepared by chemical methods. Detailed study of this chemically synthesized L-pyrrolysine has allowed us to characterize its physical properties, to study its chemical stability, and to elucidate the role of its C(4) substituent. Future applications of this synthetic L-pyrrolysine include its in vivo incorporation into recombinant proteins.
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==About this Structure==
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Reactivity and chemical synthesis of L-pyrrolysine- the 22(nd) genetically encoded amino acid.,Hao B, Zhao G, Kang PT, Soares JA, Ferguson TK, Gallucci J, Krzycki JA, Chan MK Chem Biol. 2004 Sep;11(9):1317-24. PMID:15380192<ref>PMID:15380192</ref>
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1TV4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanosarcina_barkeri Methanosarcina barkeri] with SO4 and BG3 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1TV4 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Reactivity and chemical synthesis of L-pyrrolysine- the 22(nd) genetically encoded amino acid., Hao B, Zhao G, Kang PT, Soares JA, Ferguson TK, Gallucci J, Krzycki JA, Chan MK, Chem Biol. 2004 Sep;11(9):1317-24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15380192 15380192]
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</div>
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<div class="pdbe-citations 1tv4" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Methanosarcina barkeri]]
[[Category: Methanosarcina barkeri]]
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[[Category: Single protein]]
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[[Category: Chan MK]]
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[[Category: Chan, M.K.]]
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[[Category: Ferguson TK]]
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[[Category: Ferguson, T.K.]]
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[[Category: Gallucci J]]
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[[Category: Gallucci, J.]]
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[[Category: Hao B]]
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[[Category: Hao, B.]]
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[[Category: Kang PT]]
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[[Category: Kang, P.T.]]
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[[Category: Krzycki JA]]
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[[Category: Krzycki, J.A.]]
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[[Category: Soares JA]]
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[[Category: Soares, J.A.]]
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[[Category: Zhao G]]
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[[Category: Zhao, G.]]
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[[Category: BG3]]
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[[Category: SO4]]
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[[Category: tim barrel]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 03:37:13 2007''
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Current revision

Crystal structure of the sulfite MtmB complex

PDB ID 1tv4

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