1tvm

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(New page: 200px<br /><applet load="1tvm" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tvm" /> '''NMR structure of enzyme GatB of the galactit...)
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[[Image:1tvm.gif|left|200px]]<br /><applet load="1tvm" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1tvm" />
 
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'''NMR structure of enzyme GatB of the galactitol-specific phosphoenolpyruvate-dependent phosphotransferase system'''<br />
 
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==Overview==
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==NMR structure of enzyme GatB of the galactitol-specific phosphoenolpyruvate-dependent phosphotransferase system==
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The phosphoenolpyruvate-dependent carbohydrate transport system (PTS), couples uptake with phosphorylation of a variety of carbohydrates in, prokaryotes. In this multienzyme complex, the enzyme II (EII), a, carbohydrate-specific permease, is constituted of two cytoplasmic domains, IIA and IIB, and a transmembrane channel IIC domain. Among the five, families of EIIs identified in Escherichia coli, the galactitol-specific, transporter (II(gat)) belongs to the glucitol family and is structurally, the least well-characterized. Here, we used nuclear magnetic resonance, (NMR) spectroscopy to solve the three-dimensional structure of the IIB, subunit (GatB). GatB consists of a central four-stranded parallel, beta-sheet flanked by alpha-helices on both sides; the active site, cysteine of GatB is located at the beginning of an unstructured loop, between beta1 and alpha1 that folds into a P-loop-like structure. This, structural arrangement shows similarities with other IIB subunits but also, with mammalian low molecular weight protein tyrosine phosphatases (LMW, PTPase) and arsenate reductase (ArsC). An NMR titration was performed to, identify the GatA-interacting residues.
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<StructureSection load='1tvm' size='340' side='right'caption='[[1tvm]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1tvm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TVM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TVM FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tvm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tvm OCA], [https://pdbe.org/1tvm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tvm RCSB], [https://www.ebi.ac.uk/pdbsum/1tvm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tvm ProSAT], [https://www.topsan.org/Proteins/BSGI/1tvm TOPSAN]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PTKB_ECOL6 PTKB_ECOL6] The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system is involved in galactitol transport (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tv/1tvm_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tvm ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The phosphoenolpyruvate-dependent carbohydrate transport system (PTS) couples uptake with phosphorylation of a variety of carbohydrates in prokaryotes. In this multienzyme complex, the enzyme II (EII), a carbohydrate-specific permease, is constituted of two cytoplasmic domains, IIA and IIB, and a transmembrane channel IIC domain. Among the five families of EIIs identified in Escherichia coli, the galactitol-specific transporter (II(gat)) belongs to the glucitol family and is structurally the least well-characterized. Here, we used nuclear magnetic resonance (NMR) spectroscopy to solve the three-dimensional structure of the IIB subunit (GatB). GatB consists of a central four-stranded parallel beta-sheet flanked by alpha-helices on both sides; the active site cysteine of GatB is located at the beginning of an unstructured loop between beta1 and alpha1 that folds into a P-loop-like structure. This structural arrangement shows similarities with other IIB subunits but also with mammalian low molecular weight protein tyrosine phosphatases (LMW PTPase) and arsenate reductase (ArsC). An NMR titration was performed to identify the GatA-interacting residues.
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==About this Structure==
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NMR structure of the enzyme GatB of the galactitol-specific phosphoenolpyruvate-dependent phosphotransferase system and its interaction with GatA.,Volpon L, Young CR, Matte A, Gehring K Protein Sci. 2006 Oct;15(10):2435-41. Epub 2006 Sep 8. PMID:16963640<ref>PMID:16963640</ref>
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1TVM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Active as [http://en.wikipedia.org/wiki/Protein-N(pi)-phosphohistidine--sugar_phosphotransferase Protein-N(pi)-phosphohistidine--sugar phosphotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.69 2.7.1.69] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1TVM OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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NMR structure of the enzyme GatB of the galactitol-specific phosphoenolpyruvate-dependent phosphotransferase system and its interaction with GatA., Volpon L, Young CR, Matte A, Gehring K, Protein Sci. 2006 Oct;15(10):2435-41. Epub 2006 Sep 8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16963640 16963640]
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</div>
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<div class="pdbe-citations 1tvm" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Protein-N(pi)-phosphohistidine--sugar phosphotransferase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Cygler M]]
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[[Category: BSGI, Montreal-Kingston.Bacterial.Structural.Genomics.Initiative.]]
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[[Category: Gehring K]]
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[[Category: Cygler, M.]]
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[[Category: Iannuzzi P]]
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[[Category: Gehring, K.]]
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[[Category: Lim NS]]
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[[Category: Iannuzzi, P.]]
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[[Category: Volpon L]]
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[[Category: Lim, N.S.]]
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[[Category: Young CR]]
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[[Category: Volpon, L.]]
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[[Category: Young, C.R.]]
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[[Category: bsgi]]
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[[Category: e. coli]]
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[[Category: four-stranded parallel open twisted beta sheet flanked by alpha helices on both sides]]
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[[Category: montreal-kingston bacterial structural genomics initiative]]
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[[Category: p-loop]]
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[[Category: phosphotransferase system (pts)]]
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[[Category: structural genomics]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 03:37:47 2007''
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Current revision

NMR structure of enzyme GatB of the galactitol-specific phosphoenolpyruvate-dependent phosphotransferase system

PDB ID 1tvm

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