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1txy

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(New page: 200px<br /><applet load="1txy" size="450" color="white" frame="true" align="right" spinBox="true" caption="1txy, resolution 2.00&Aring;" /> '''E. coli PriB'''<br /...)
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[[Image:1txy.gif|left|200px]]<br /><applet load="1txy" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1txy, resolution 2.00&Aring;" />
 
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'''E. coli PriB'''<br />
 
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==Overview==
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==E. coli PriB==
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Maintenance of genome stability following DNA damage requires, origin-independent reinitiation of DNA replication at repaired replication, forks. In E. coli, PriA, PriB, PriC, and DnaT play critical roles in, recognizing repaired replication forks and reloading the replisome onto, the template to reinitiate DNA replication. Here, we report the 2.0 A, resolution crystal structure of E. coli PriB, revealing a dimer that, consists of a single structural domain formed by two, oligonucleotide/oligosaccharide binding (OB) folds. Structural similarity, of PriB to single-stranded DNA binding proteins reveals insights into its, mechanisms of DNA binding. The structure further establishes a putative, protein interaction surface that may contribute to the role of PriB in, primosome assembly by facilitating interactions with PriA and DnaT. This, is the first high-resolution structure of a protein involved in, oriC-independent replisome loading and provides unique insight into, mechanisms of replication restart in E. coli.
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<StructureSection load='1txy' size='340' side='right'caption='[[1txy]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1txy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TXY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TXY FirstGlance]. <br>
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1TXY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1TXY OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1txy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1txy OCA], [https://pdbe.org/1txy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1txy RCSB], [https://www.ebi.ac.uk/pdbsum/1txy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1txy ProSAT]</span></td></tr>
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Crystal structure of PriB, a component of the Escherichia coli replication restart primosome., Lopper M, Holton JM, Keck JL, Structure. 2004 Nov;12(11):1967-75. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15530361 15530361]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PRIB_ECOLI PRIB_ECOLI] Binds single-stranded DNA at the primosome assembly site (PAS). During primosome assembly it facilitates the complex formation between PriA and DnaT.<ref>PMID:1856227</ref> <ref>PMID:1646811</ref> <ref>PMID:8366072</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tx/1txy_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1txy ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Holton, J.M.]]
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[[Category: Holton JM]]
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[[Category: Keck, J.L.]]
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[[Category: Keck JL]]
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[[Category: Lopper, M.]]
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[[Category: Lopper M]]
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[[Category: dimer]]
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[[Category: ob fold]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 03:41:00 2007''
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Current revision

E. coli PriB

PDB ID 1txy

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