1tzp

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(New page: 200px<br /><applet load="1tzp" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tzp, resolution 1.40&Aring;" /> '''MEPA, inactive form ...)
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[[Image:1tzp.gif|left|200px]]<br /><applet load="1tzp" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1tzp, resolution 1.40&Aring;" />
 
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'''MEPA, inactive form without ZN in P21'''<br />
 
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==Overview==
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==MEPA, inactive form without ZN in P21==
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LAS enzymes are a group of metallopeptidases that share an active site, architecture and a core folding motif and have been named according to the, group members lysostaphin, D-Ala-D-Ala carboxypeptidase and sonic, hedgehog. Escherichia coli MepA is a periplasmic, penicillin-insensitive, murein endopeptidase that cleaves the D-alanyl-meso-2,6-diamino-pimelyl, amide bond in E. coli peptidoglycan. The enzyme lacks sequence similarity, with other peptidases, and is currently classified as a peptidase of, unknown fold and catalytic class in all major data bases. Here, we build, on our observation that two motifs, characteristic of the newly described, LAS group of metallopeptidases, are conserved in MepA-type sequences. We, demonstrate that recombinant E. coli MepA is sensitive to metal chelators, and that mutations in the predicted Zn2+ ligands His-113, Asp-120, and, His-211 inactivate the enzyme. Moreover, we present the crystal structure, of MepA. The active site of the enzyme is most similar to the active sites, of lysostaphin and D-Ala-D-Ala carboxypeptidase, and the fold is most, closely related to the N-domain of sonic hedgehog. We conclude that, MepA-type peptidases are LAS enzymes.
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<StructureSection load='1tzp' size='340' side='right'caption='[[1tzp]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1tzp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TZP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TZP FirstGlance]. <br>
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1TZP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with SO4 and BU1 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1TZP OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BU1:1,4-BUTANEDIOL'>BU1</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tzp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tzp OCA], [https://pdbe.org/1tzp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tzp RCSB], [https://www.ebi.ac.uk/pdbsum/1tzp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tzp ProSAT]</span></td></tr>
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Peptidoglycan amidase MepA is a LAS metallopeptidase., Marcyjaniak M, Odintsov SG, Sabala I, Bochtler M, J Biol Chem. 2004 Oct 15;279(42):43982-9. Epub 2004 Jul 29. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15292190 15292190]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MEPA_ECOLI MEPA_ECOLI] Involved in the removal of murein from the sacculus. May also facilitate integration of nascent murein strands into the sacculus by cleaving the peptide bonds between neighboring strands in mature murein.<ref>PMID:15292190</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tz/1tzp_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tzp ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Bochtler, M.]]
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[[Category: Bochtler M]]
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[[Category: Marcyjaniak, M.]]
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[[Category: Marcyjaniak M]]
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[[Category: Odintsov, S.G.]]
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[[Category: Odintsov SG]]
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[[Category: Sabala, I.]]
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[[Category: Sabala I]]
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[[Category: BU1]]
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[[Category: SO4]]
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[[Category: las enzyme]]
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[[Category: metallopeptidase]]
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[[Category: peptidoglycan hydrolase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 03:43:43 2007''
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Current revision

MEPA, inactive form without ZN in P21

PDB ID 1tzp

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