1ipe

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{{Seed}}
 
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[[Image:1ipe.png|left|200px]]
 
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==TROPINONE REDUCTASE-II COMPLEXED WITH NADPH==
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The line below this paragraph, containing "STRUCTURE_1ipe", creates the "Structure Box" on the page.
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<StructureSection load='1ipe' size='340' side='right'caption='[[1ipe]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1ipe]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Datura_stramonium Datura stramonium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IPE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IPE FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr>
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{{STRUCTURE_1ipe| PDB=1ipe | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ipe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ipe OCA], [https://pdbe.org/1ipe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ipe RCSB], [https://www.ebi.ac.uk/pdbsum/1ipe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ipe ProSAT], [https://www.topsan.org/Proteins/RSGI/1ipe TOPSAN]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TRN2_DATST TRN2_DATST] Catalyzes the stereospecific reduction of tropinone to pseudotropine.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ip/1ipe_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ipe ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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To understand the catalytic mechanism of an enzyme, it is crucial to determine the crystallographic structures corresponding to the individual reaction steps. Here, we report two crystal structures of enzyme-substrate complexes prior to reaction initiation: tropinone reductase-II (TR-II)-NADPH and TR-II-NADPH-tropinone complexes, determined from the identical crystals. A combination of two kinetic crystallographic techniques, a continuous flow of the substrates and Laue diffraction measurements, enabled us to capture the transit structures prior to the reaction proceeding. A structure comparison of the enzyme-substrate complex elucidated in this study with the enzyme-product complex in our previous study indicates that one of the substrates, tropinone, is rotated relative to the product so as to make the spatial organization in the active site favorable for the reaction to proceed. Side chains of the residues in the active site also alter their conformations to keep the complementarity of the space for the substrate or the product and to assist the rotational movement.
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===TROPINONE REDUCTASE-II COMPLEXED WITH NADPH===
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Capturing enzyme structure prior to reaction initiation: tropinone reductase-II-substrate complexes.,Yamashita A, Endo M, Higashi T, Nakatsu T, Yamada Y, Oda J, Kato H Biochemistry. 2003 May 20;42(19):5566-73. PMID:12741812<ref>PMID:12741812</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_12741812}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1ipe" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 12741812 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_12741812}}
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__TOC__
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</StructureSection>
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==About this Structure==
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1IPE is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Datura_stramonium Datura stramonium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IPE OCA].
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==Reference==
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Capturing enzyme structure prior to reaction initiation: tropinone reductase-II-substrate complexes., Yamashita A, Endo M, Higashi T, Nakatsu T, Yamada Y, Oda J, Kato H, Biochemistry. 2003 May 20;42(19):5566-73. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12741812 12741812]
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[[Category: Datura stramonium]]
[[Category: Datura stramonium]]
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[[Category: Tropinone reductase II]]
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[[Category: Large Structures]]
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[[Category: Endo, M.]]
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[[Category: Endo M]]
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[[Category: Higashi, T.]]
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[[Category: Higashi T]]
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[[Category: Kato, H.]]
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[[Category: Kato H]]
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[[Category: Nakatsu, T.]]
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[[Category: Nakatsu T]]
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[[Category: Oda, J.]]
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[[Category: Oda J]]
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[[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]]
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[[Category: Yamada Y]]
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[[Category: Yamada, Y.]]
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[[Category: Yamashita A]]
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[[Category: Yamashita, A.]]
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[[Category: Laue diffraction]]
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[[Category: Oxidoreductase]]
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[[Category: Reduction of tropinone to pseudotropine]]
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[[Category: Riken structural genomics/proteomics initiative]]
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[[Category: Rsgi]]
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[[Category: Short-chain dehydrogenase]]
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[[Category: Structural genomic]]
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[[Category: Tropane alkaloid biosynthesis]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Nov 16 13:49:03 2008''
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Current revision

TROPINONE REDUCTASE-II COMPLEXED WITH NADPH

PDB ID 1ipe

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