This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1m33

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (08:30, 10 April 2024) (edit) (undo)
 
(10 intermediate revisions not shown.)
Line 1: Line 1:
-
{{Seed}}
 
-
[[Image:1m33.png|left|200px]]
 
-
<!--
+
==Crystal Structure of BioH at 1.7 A==
-
The line below this paragraph, containing "STRUCTURE_1m33", creates the "Structure Box" on the page.
+
<StructureSection load='1m33' size='340' side='right'caption='[[1m33]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[1m33]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M33 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1M33 FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3OH:3-HYDROXY-PROPANOIC+ACID'>3OH</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-
{{STRUCTURE_1m33| PDB=1m33 | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1m33 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m33 OCA], [https://pdbe.org/1m33 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1m33 RCSB], [https://www.ebi.ac.uk/pdbsum/1m33 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1m33 ProSAT], [https://www.topsan.org/Proteins/MCSG/1m33 TOPSAN]</span></td></tr>
-
 
+
</table>
-
===Crystal Structure of BioH at 1.7 A===
+
== Function ==
-
 
+
[https://www.uniprot.org/uniprot/BIOH_ECOLI BIOH_ECOLI] The physiological role of BioH is to remove the methyl group introduced by BioC when the pimeloyl moiety is complete. It allows to synthesize pimeloyl-ACP via the fatty acid synthetic pathway through the hydrolysis of the ester bonds of pimeloyl-ACP esters. E.coli employs a methylation and demethylation strategy to allow elongation of a temporarily disguised malonate moiety to a pimelate moiety by the fatty acid synthetic enzymes. BioH shows a preference for short chain fatty acid esters (acyl chain length of up to 6 carbons) and short chain p-nitrophenyl esters. Also displays a weak thioesterase activity. Can form a complex with CoA, and may be involved in the condensation of CoA and pimelic acid into pimeloyl-CoA, a precursor in biotin biosynthesis.<ref>PMID:11904168</ref> <ref>PMID:17625941</ref> <ref>PMID:20693992</ref> <ref>PMID:12732651</ref> Catalyzes the hydrolysis of the methyl ester bond of dimethylbutyryl-S-methyl mercaptopropionate (DMB-S-MMP) to yield dimethylbutyryl mercaptopropionic acid (DMBS-MPA) during the biocatalytic conversion of simvastin acid from monacolin J acid. Can also use acyl carriers such as dimethylbutyryl-S-ethyl mercaptopropionate (DMB-S-EMP) and dimethylbutyryl-S-methyl thioglycolate (DMB-S-MTG) as the thioester substrates.<ref>PMID:11904168</ref> <ref>PMID:17625941</ref> <ref>PMID:20693992</ref> <ref>PMID:12732651</ref>
-
 
+
== Evolutionary Conservation ==
-
<!--
+
[[Image:Consurf_key_small.gif|200px|right]]
-
The line below this paragraph, {{ABSTRACT_PUBMED_12732651}}, adds the Publication Abstract to the page
+
Check<jmol>
-
(as it appears on PubMed at http://www.pubmed.gov), where 12732651 is the PubMed ID number.
+
<jmolCheckbox>
-
-->
+
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/m3/1m33_consurf.spt"</scriptWhenChecked>
-
{{ABSTRACT_PUBMED_12732651}}
+
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-
 
+
<text>to colour the structure by Evolutionary Conservation</text>
-
==About this Structure==
+
</jmolCheckbox>
-
1M33 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M33 OCA].
+
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1m33 ConSurf].
-
 
+
<div style="clear:both"></div>
-
==Reference==
+
== References ==
-
Integrating structure, bioinformatics, and enzymology to discover function: BioH, a new carboxylesterase from Escherichia coli., Sanishvili R, Yakunin AF, Laskowski RA, Skarina T, Evdokimova E, Doherty-Kirby A, Lajoie GA, Thornton JM, Arrowsmith CH, Savchenko A, Joachimiak A, Edwards AM, J Biol Chem. 2003 Jul 11;278(28):26039-45. Epub 2003 May 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12732651 12732651]
+
<references/>
 +
__TOC__
 +
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
-
[[Category: Edwards, A.]]
+
[[Category: Large Structures]]
-
[[Category: Joachimiak, A.]]
+
[[Category: Edwards A]]
-
[[Category: MCSG, Midwest Center for Structural Genomics.]]
+
[[Category: Joachimiak A]]
-
[[Category: Sanishvili, R.]]
+
[[Category: Sanishvili R]]
-
[[Category: Savchenko, A.]]
+
[[Category: Savchenko A]]
-
[[Category: Skarina, T.]]
+
[[Category: Skarina T]]
-
[[Category: Yakunin, A.]]
+
[[Category: Yakunin A]]
-
[[Category: Alpha-betta-alpha sandwich]]
+
-
[[Category: Mcsg]]
+
-
[[Category: Midwest center for structural genomic]]
+
-
[[Category: Protein structure initiative]]
+
-
[[Category: Psi]]
+
-
[[Category: Structural genomic]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Nov 16 14:11:42 2008''
+

Current revision

Crystal Structure of BioH at 1.7 A

PDB ID 1m33

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1m33"
Personal tools