1u58

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(New page: 200px<br /><applet load="1u58" size="450" color="white" frame="true" align="right" spinBox="true" caption="1u58, resolution 1.90&Aring;" /> '''Crystal structure of...)
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[[Image:1u58.gif|left|200px]]<br /><applet load="1u58" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1u58, resolution 1.90&Aring;" />
 
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'''Crystal structure of the murine cytomegalovirus MHC-I homolog m144'''<br />
 
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==Overview==
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==Crystal structure of the murine cytomegalovirus MHC-I homolog m144==
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Large DNA viruses of the herpesvirus family produce proteins that mimic, host MHC-I molecules as part of their immunoevasive strategy. The m144, glycoprotein, expressed by murine cytomegalovirus, is thought to be an, MHC-I homolog whose expression prolongs viral survival in vivo by, preventing natural killer cell activation. To explore the structural basis, of this m144 function, we have determined the three-dimensional structure, of an m144/beta2-microglobulin (beta2m) complex at 1.9A resolution. This, structure reveals the canonical features of MHC-I molecules including, readily identifiable alpha1, alpha2, and alpha3 domains. A unique, disulfide bond links the alpha1 helix to the beta-sheet floor, explaining, the known thermal stability of m144. Close juxtaposition of the alpha1 and, alpha2 helices and the lack of critical residues that normally contribute, to anchoring the peptide N and C termini eliminates peptide binding. A, region of 13 amino acid residues, corresponding to the amino-terminal, portion of the alpha2 helix, is missing in the electron density map, suggesting an area of structural flexibility that may be involved in, ligand binding.
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<StructureSection load='1u58' size='340' side='right'caption='[[1u58]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1u58]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Murid_betaherpesvirus_1 Murid betaherpesvirus 1] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U58 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1U58 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1u58 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u58 OCA], [https://pdbe.org/1u58 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1u58 RCSB], [https://www.ebi.ac.uk/pdbsum/1u58 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1u58 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/A2Q6L5_MUHVK A2Q6L5_MUHVK]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/u5/1u58_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1u58 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Large DNA viruses of the herpesvirus family produce proteins that mimic host MHC-I molecules as part of their immunoevasive strategy. The m144 glycoprotein, expressed by murine cytomegalovirus, is thought to be an MHC-I homolog whose expression prolongs viral survival in vivo by preventing natural killer cell activation. To explore the structural basis of this m144 function, we have determined the three-dimensional structure of an m144/beta2-microglobulin (beta2m) complex at 1.9A resolution. This structure reveals the canonical features of MHC-I molecules including readily identifiable alpha1, alpha2, and alpha3 domains. A unique disulfide bond links the alpha1 helix to the beta-sheet floor, explaining the known thermal stability of m144. Close juxtaposition of the alpha1 and alpha2 helices and the lack of critical residues that normally contribute to anchoring the peptide N and C termini eliminates peptide binding. A region of 13 amino acid residues, corresponding to the amino-terminal portion of the alpha2 helix, is missing in the electron density map, suggesting an area of structural flexibility that may be involved in ligand binding.
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==About this Structure==
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Crystal structure of the murine cytomegalovirus MHC-I homolog m144.,Natarajan K, Hicks A, Mans J, Robinson H, Guan R, Mariuzza RA, Margulies DH J Mol Biol. 2006 Apr 21;358(1):157-71. Epub 2006 Feb 9. PMID:16500675<ref>PMID:16500675</ref>
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1U58 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Murid_herpesvirus_1 Murid herpesvirus 1] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1U58 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Crystal structure of the murine cytomegalovirus MHC-I homolog m144., Natarajan K, Hicks A, Mans J, Robinson H, Guan R, Mariuzza RA, Margulies DH, J Mol Biol. 2006 Apr 21;358(1):157-71. Epub 2006 Feb 9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16500675 16500675]
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</div>
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[[Category: Murid herpesvirus 1]]
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<div class="pdbe-citations 1u58" style="background-color:#fffaf0;"></div>
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[[Category: Mus musculus]]
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[[Category: Single protein]]
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[[Category: Guan, R.]]
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[[Category: Hicks, A.]]
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[[Category: Margulies, D.H.]]
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[[Category: Natarajan, K.]]
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[[Category: Robinson, H.]]
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[[Category: beta-2m]]
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[[Category: m144]]
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[[Category: mcmv]]
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[[Category: mhc-i homolog]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 03:51:01 2007''
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==See Also==
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*[[Beta-2 microglobulin 3D structures|Beta-2 microglobulin 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Murid betaherpesvirus 1]]
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[[Category: Mus musculus]]
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[[Category: Guan R]]
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[[Category: Hicks A]]
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[[Category: Margulies DH]]
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[[Category: Natarajan K]]
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[[Category: Robinson H]]

Current revision

Crystal structure of the murine cytomegalovirus MHC-I homolog m144

PDB ID 1u58

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