This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1u5y

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1u5y" size="450" color="white" frame="true" align="right" spinBox="true" caption="1u5y, resolution 2.30&Aring;" /> '''Crystal structure of...)
Current revision (06:16, 3 April 2024) (edit) (undo)
 
(14 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1u5y.gif|left|200px]]<br /><applet load="1u5y" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1u5y, resolution 2.30&Aring;" />
 
-
'''Crystal structure of murine APRIL, pH 8.0'''<br />
 
-
==Overview==
+
==Crystal structure of murine APRIL, pH 8.0==
-
A proliferation-inducing ligand (APRIL) is a TNF-like cytokine that, stimulates tumor cell growth. Within the TNF ligand superfamily, APRIL is, most similar to B-cell activation factor (BAFF) with which it shares 30%, sequence identity. APRIL binds the receptors B-cell maturation antigen, (BCMA) and TACI with high affinity; both of these receptors have also been, shown to bind BAFF, although BCMA has significantly higher affinity for, APRIL than BAFF. Determination of the crystal structure of APRIL from, three crystallization conditions at resolutions of 1.8-2.4A over a pH, range from 5.0 to 8.5 reveals a compact trimeric ligand with a backbone, fold very similar to that of BAFF (1.1A RMSD over 122 structurally, equivalent Calpha atoms), with the exception of differences in the AA' and, DE loop regions. Whereas BAFF has been shown to form 20-trimer assemblies, under certain conditions, the molecular determinants required for, BAFF-like assemblies are absent in the APRIL structure. No crystal packing, suggestive of the formation of higher-order assemblies is seen in any of, the crystal forms nor does the structure vary significantly between pH 5.0, and 8.5. Modeling of the APRIL-BCMA complex shows the resulting interface, is in agreement with mutagenesis data.
+
<StructureSection load='1u5y' size='340' side='right'caption='[[1u5y]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1u5y]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U5Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1U5Y FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1u5y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u5y OCA], [https://pdbe.org/1u5y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1u5y RCSB], [https://www.ebi.ac.uk/pdbsum/1u5y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1u5y ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/TNF13_MOUSE TNF13_MOUSE]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/u5/1u5y_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1u5y ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
1U5Y is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1U5Y OCA].
+
*[[Tumor necrosis factor ligand superfamily 3D structures|Tumor necrosis factor ligand superfamily 3D structures]]
-
 
+
__TOC__
-
==Reference==
+
</StructureSection>
-
The crystal structure of a proliferation-inducing ligand, APRIL., Wallweber HJ, Compaan DM, Starovasnik MA, Hymowitz SG, J Mol Biol. 2004 Oct 15;343(2):283-90. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15451660 15451660]
+
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
-
[[Category: Single protein]]
+
[[Category: Compaan DM]]
-
[[Category: Compaan, D.M.]]
+
[[Category: Hymowitz SG]]
-
[[Category: Hymowitz, S.G.]]
+
[[Category: Starovasnik MA]]
-
[[Category: Starovasnik, M.A.]]
+
[[Category: Wallweber HJ]]
-
[[Category: Wallweber, H.J.]]
+
-
[[Category: cytokine]]
+
-
[[Category: jelly-roll]]
+
-
[[Category: tnfsf]]
+
-
[[Category: trimer]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 03:52:14 2007''
+

Current revision

Crystal structure of murine APRIL, pH 8.0

PDB ID 1u5y

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1u5y"
Personal tools