1u8x

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(New page: 200px<br /><applet load="1u8x" size="450" color="white" frame="true" align="right" spinBox="true" caption="1u8x, resolution 2.05&Aring;" /> '''CRYSTAL STRUCTURE OF...)
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[[Image:1u8x.gif|left|200px]]<br /><applet load="1u8x" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1u8x, resolution 2.05&Aring;" />
 
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'''CRYSTAL STRUCTURE OF GLVA FROM BACILLUS SUBTILIS, A METAL-REQUIRING, NAD-DEPENDENT 6-PHOSPHO-ALPHA-GLUCOSIDASE'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF GLVA FROM BACILLUS SUBTILIS, A METAL-REQUIRING, NAD-DEPENDENT 6-PHOSPHO-ALPHA-GLUCOSIDASE==
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GlvA, a 6-phospho-alpha-glucosidase from Bacillus subtilis, catalyzes the, hydrolysis of maltose-6'-phosphate and belongs to glycoside hydrolase, family GH4. GH4 enzymes are unique in their requirement for NAD(H) and a, divalent metal for activity. We have determined the crystal structure of, GlvA in complex with its ligands to 2.05 A resolution. Analyses of the, active site architecture, in conjunction with mechanistic studies and, precedent from the nucleotide diphosphate hexose dehydratases and other, systems, suggest a novel mechanism of glycoside hydrolysis by GlvA that, involves both the NAD(H) and the metal.
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<StructureSection load='1u8x' size='340' side='right'caption='[[1u8x]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1u8x]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1nrh 1nrh]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U8X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1U8X FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=G6P:ALPHA-D-GLUCOSE-6-PHOSPHATE'>G6P</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1u8x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u8x OCA], [https://pdbe.org/1u8x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1u8x RCSB], [https://www.ebi.ac.uk/pdbsum/1u8x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1u8x ProSAT], [https://www.topsan.org/Proteins/MCSG/1u8x TOPSAN]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GLVA_BACSU GLVA_BACSU] Hydrolyzes maltose-6'-phosphate and trehalose-6'-phosphate. Is involved in the catabolism of alpha-glycosides accumulated via a phosphoenolpyruvate-dependent maltose phosphotransferase system (PEP-PTS). Is also able to significantly catalyze the hydrolysis of both 6-phospho-alpha- and 6-phospho-beta-glucosides containing activated leaving groups such as p-nitrophenol and does so with retention and inversion, respectively, of the substrate anomeric configuration.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/u8/1u8x_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1u8x ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1U8X is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with G6P, MN and NAD as [http://en.wikipedia.org/wiki/ligands ligands]. This structure superseeds the now removed PDB entry 1NRH. Active as [http://en.wikipedia.org/wiki/Maltose-6'-phosphate_glucosidase Maltose-6'-phosphate glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.122 3.2.1.122] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1U8X OCA].
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*[[Alpha-glucosidase 3D structures|Alpha-glucosidase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Novel catalytic mechanism of glycoside hydrolysis based on the structure of an NAD+/Mn2+ -dependent phospho-alpha-glucosidase from Bacillus subtilis., Rajan SS, Yang X, Collart F, Yip VL, Withers SG, Varrot A, Thompson J, Davies GJ, Anderson WF, Structure. 2004 Sep;12(9):1619-29. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15341727 15341727]
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[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
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[[Category: Maltose-6'-phosphate glucosidase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Anderson WF]]
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[[Category: Anderson, W.F.]]
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[[Category: Collart F]]
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[[Category: Collart, F.]]
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[[Category: Rajan SS]]
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[[Category: MCSG, Midwest.Center.for.Structural.Genomics.]]
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[[Category: Yang X]]
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[[Category: Rajan, S.S.]]
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[[Category: Yang, X.]]
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[[Category: G6P]]
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[[Category: MN]]
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[[Category: NAD]]
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[[Category: glucosidase]]
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[[Category: mcsg]]
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[[Category: midwest center for structural genomics]]
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[[Category: nad-dependent]]
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[[Category: protein structure initiative]]
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[[Category: psi]]
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[[Category: structural genomics]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 03:55:48 2007''
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Current revision

CRYSTAL STRUCTURE OF GLVA FROM BACILLUS SUBTILIS, A METAL-REQUIRING, NAD-DEPENDENT 6-PHOSPHO-ALPHA-GLUCOSIDASE

PDB ID 1u8x

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