1ua5
From Proteopedia
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(New page: 200px<br /><applet load="1ua5" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ua5, resolution 2.50Å" /> '''Non-fusion GST from ...) |
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| - | [[Image:1ua5.jpg|left|200px]]<br /><applet load="1ua5" size="450" color="white" frame="true" align="right" spinBox="true" | ||
| - | caption="1ua5, resolution 2.50Å" /> | ||
| - | '''Non-fusion GST from S. japonicum in complex with glutathione'''<br /> | ||
| - | == | + | ==Non-fusion GST from S. japonicum in complex with glutathione== |
| - | + | <StructureSection load='1ua5' size='340' side='right'caption='[[1ua5]], [[Resolution|resolution]] 2.50Å' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[1ua5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Schistosoma_japonicum Schistosoma japonicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UA5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UA5 FirstGlance]. <br> | |
| - | [ | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> |
| - | [ | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | [[ | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ua5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ua5 OCA], [https://pdbe.org/1ua5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ua5 RCSB], [https://www.ebi.ac.uk/pdbsum/1ua5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ua5 ProSAT]</span></td></tr> |
| - | [ | + | </table> |
| - | [[ | + | == Function == |
| - | [ | + | [https://www.uniprot.org/uniprot/GST26_SCHJA GST26_SCHJA] Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut. |
| - | + | == Evolutionary Conservation == | |
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ua/1ua5_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ua5 ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Schistosoma japonicum glutathione S-transferase (SjGST) is a common fusion tag in recombinant protein production, and its 3-dimensional structure has been studied in the context of drug design. We have determined the crystal structure of non-fused SjGST complexed with glutathione, and compare it to complexes between glutathione and SjGST fusion proteins. | ||
| - | + | Crystal structure of non-fused glutathione S-transferase from Schistosoma japonicum in complex with glutathione.,Kursula I, Heape AM, Kursula P Protein Pept Lett. 2005 Oct;12(7):709-12. doi: 10.2174/0929866054696154. PMID:16522189<ref>PMID:16522189</ref> | |
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 1ua5" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Glutathione S-transferase 3D structures|Glutathione S-transferase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Schistosoma japonicum]] | ||
| + | [[Category: Heape AM]] | ||
| + | [[Category: Kursula I]] | ||
| + | [[Category: Kursula P]] | ||
Current revision
Non-fusion GST from S. japonicum in complex with glutathione
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