1ri5

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{{Seed}}
 
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[[Image:1ri5.png|left|200px]]
 
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==Structure and mechanism of mRNA cap (guanine N-7) methyltransferase==
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The line below this paragraph, containing "STRUCTURE_1ri5", creates the "Structure Box" on the page.
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<StructureSection load='1ri5' size='340' side='right'caption='[[1ri5]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1ri5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Encephalitozoon_cuniculi Encephalitozoon cuniculi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RI5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RI5 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ri5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ri5 OCA], [https://pdbe.org/1ri5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ri5 RCSB], [https://www.ebi.ac.uk/pdbsum/1ri5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ri5 ProSAT], [https://www.topsan.org/Proteins/NYSGXRC/1ri5 TOPSAN]</span></td></tr>
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{{STRUCTURE_1ri5| PDB=1ri5 | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MCES_ENCCU MCES_ENCCU] mRNA-capping methyltransferase that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs. Binds RNA containing 5'-terminal GpppC.<ref>PMID:15760890</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ri/1ri5_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ri5 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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A suite of crystal structures is reported for a cellular mRNA cap (guanine-N7) methyltransferase in complex with AdoMet, AdoHcy, and the cap guanylate. Superposition of ligand complexes suggests an in-line mechanism of methyl transfer, albeit without direct contacts between the enzyme and either the N7 atom of guanine (the attacking nucleophile), the methyl carbon of AdoMet, or the sulfur of AdoMet/AdoHcy (the leaving group). The structures indicate that catalysis of cap N7 methylation is accomplished by optimizing proximity and orientation of the substrates, assisted by a favorable electrostatic environment. The enzyme-ligand structures, together with new mutational data, fully account for the biochemical specificity of the cap guanine-N7 methylation reaction, an essential and defining step of eukaryotic mRNA synthesis.
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===Structure and mechanism of mRNA cap (guanine N-7) methyltransferase===
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Structure and mechanism of mRNA cap (guanine-N7) methyltransferase.,Fabrega C, Hausmann S, Shen V, Shuman S, Lima CD Mol Cell. 2004 Jan 16;13(1):77-89. PMID:14731396<ref>PMID:14731396</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_14731396}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1ri5" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 14731396 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_14731396}}
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__TOC__
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</StructureSection>
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==About this Structure==
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1RI5 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Encephalitozoon_cuniculi Encephalitozoon cuniculi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RI5 OCA].
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==Reference==
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Structure and mechanism of mRNA cap (guanine-N7) methyltransferase., Fabrega C, Hausmann S, Shen V, Shuman S, Lima CD, Mol Cell. 2004 Jan 16;13(1):77-89. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14731396 14731396]
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[[Category: Encephalitozoon cuniculi]]
[[Category: Encephalitozoon cuniculi]]
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[[Category: Burley, S K.]]
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[[Category: Large Structures]]
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[[Category: Fabrega, C.]]
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[[Category: Burley SK]]
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[[Category: Hausmann, S.]]
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[[Category: Fabrega C]]
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[[Category: Lima, C D.]]
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[[Category: Hausmann S]]
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[[Category: NYSGXRC, New York Structural GenomiX Research Consortium.]]
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[[Category: Lima CD]]
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[[Category: Shen, V.]]
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[[Category: Shen V]]
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[[Category: Shuman, S.]]
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[[Category: Shuman S]]
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[[Category: Cap]]
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[[Category: M7g]]
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[[Category: Messenger rna cap]]
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[[Category: Methyltransferase]]
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[[Category: New york structural genomix research consortium]]
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[[Category: Nysgxrc]]
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[[Category: Protein structure initiative]]
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[[Category: Psi]]
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[[Category: Rna]]
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[[Category: Structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Nov 16 18:34:58 2008''
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Current revision

Structure and mechanism of mRNA cap (guanine N-7) methyltransferase

PDB ID 1ri5

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