1udv

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(New page: 200px<br /><applet load="1udv" size="450" color="white" frame="true" align="right" spinBox="true" caption="1udv, resolution 1.85&Aring;" /> '''Crystal structure of...)
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[[Image:1udv.jpg|left|200px]]<br /><applet load="1udv" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1udv, resolution 1.85&Aring;" />
 
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'''Crystal structure of the hyperthermophilic archaeal dna-binding protein Sso10b2 at 1.85 A'''<br />
 
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==Overview==
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==Crystal structure of the hyperthermophilic archaeal dna-binding protein Sso10b2 at 1.85 A==
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The crystal structure of a small, basic DNA binding protein, Sso10b2, from, the thermoacidophilic archaeon Sulfolobus solfataricus was determined by, the Zn multiwavelength anomalous diffraction method and refined to 1.85 A, resolution. The 89-amino-acid protein adopts a betaalphabetaalphabetabeta, topology. The structure is similar to that of Sso10b1 (also called Alba), from the same organism. However, Sso10b2 contains an arginine-rich loop, RDRRR motif, which may play an important role in nucleic acid binding., There are two independent Sso10b2 proteins in the asymmetric unit, and a, plausible stable dimer could be deduced from the crystal structure., Topology comparison revealed that Sso10b2 is similar to several, RNA-binding proteins, including IF3-C, YhhP, and DNase I. Models of the, Sso10b2 dimer bound to either B-DNA or A-DNA have been constructed.
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<StructureSection load='1udv' size='340' side='right'caption='[[1udv]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1udv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus_P2 Saccharolobus solfataricus P2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UDV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UDV FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1udv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1udv OCA], [https://pdbe.org/1udv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1udv RCSB], [https://www.ebi.ac.uk/pdbsum/1udv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1udv ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ALBA2_SACS2 ALBA2_SACS2] Binds double-stranded DNA tightly but without sequence specificity. It is distributed uniformly and abundantly on the chromosome, suggesting a role in chromatin architecture. However, it does not significantly compact DNA. Binds rRNA and mRNA in vivo. May play a role in maintaining the structural and functional stability of RNA, and, perhaps, ribosomes (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ud/1udv_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1udv ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structure of a small, basic DNA binding protein, Sso10b2, from the thermoacidophilic archaeon Sulfolobus solfataricus was determined by the Zn multiwavelength anomalous diffraction method and refined to 1.85 A resolution. The 89-amino-acid protein adopts a betaalphabetaalphabetabeta topology. The structure is similar to that of Sso10b1 (also called Alba) from the same organism. However, Sso10b2 contains an arginine-rich loop RDRRR motif, which may play an important role in nucleic acid binding. There are two independent Sso10b2 proteins in the asymmetric unit, and a plausible stable dimer could be deduced from the crystal structure. Topology comparison revealed that Sso10b2 is similar to several RNA-binding proteins, including IF3-C, YhhP, and DNase I. Models of the Sso10b2 dimer bound to either B-DNA or A-DNA have been constructed.
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==About this Structure==
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Crystal structure of the hyperthermophilic archaeal DNA-binding protein Sso10b2 at a resolution of 1.85 Angstroms.,Chou CC, Lin TW, Chen CY, Wang AH J Bacteriol. 2003 Jul;185(14):4066-73. PMID:12837780<ref>PMID:12837780</ref>
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1UDV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UDV OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Crystal structure of the hyperthermophilic archaeal DNA-binding protein Sso10b2 at a resolution of 1.85 Angstroms., Chou CC, Lin TW, Chen CY, Wang AH, J Bacteriol. 2003 Jul;185(14):4066-73. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12837780 12837780]
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</div>
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[[Category: Single protein]]
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<div class="pdbe-citations 1udv" style="background-color:#fffaf0;"></div>
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[[Category: Sulfolobus solfataricus]]
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== References ==
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[[Category: Chen, C.Y.]]
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<references/>
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[[Category: Chou, C.C.]]
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__TOC__
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[[Category: Lin, T.W.]]
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</StructureSection>
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[[Category: Wang, A.H.J.]]
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[[Category: Large Structures]]
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[[Category: ZN]]
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[[Category: Saccharolobus solfataricus P2]]
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[[Category: dna binding protein]]
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[[Category: Chen C-Y]]
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[[Category: Chou C-C]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 04:03:14 2007''
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[[Category: Lin T-W]]
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[[Category: Wang AHJ]]

Current revision

Crystal structure of the hyperthermophilic archaeal dna-binding protein Sso10b2 at 1.85 A

PDB ID 1udv

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