1uhb

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(New page: 200px<br /><applet load="1uhb" size="450" color="white" frame="true" align="right" spinBox="true" caption="1uhb, resolution 2.15&Aring;" /> '''Crystal structure of...)
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[[Image:1uhb.jpg|left|200px]]<br /><applet load="1uhb" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1uhb, resolution 2.15&Aring;" />
 
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'''Crystal structure of porcine alpha trypsin bound with auto catalyticaly produced native peptide at 2.15 A resolution'''<br />
 
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==Overview==
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==Crystal structure of porcine alpha trypsin bound with auto catalyticaly produced native peptide at 2.15 A resolution==
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Trypsin, a serine protease enzyme plays a pivotal role in digestion and is, autocatalytic. The crystal structure of a complex formed between porcine, trypsin and an auto catalytically produced peptide is reported here. This, complex shows a reduction in enzyme activity as compared to native, beta-trypsin. The nonapeptide has a lysine, which is recognized by Asp 189, at the specificity pocket. The auto catalytically produced native, nonapeptide is bound at the active site cleft like other trypsin, inhibitors but the important interactions with the oxyanion hole are, absent. The peptide covers only a part of the active site cleft and hence, the enzyme activity is reduced rather than being inhibited.
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<StructureSection load='1uhb' size='340' side='right'caption='[[1uhb]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1uhb]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UHB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UHB FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1uhb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uhb OCA], [https://pdbe.org/1uhb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1uhb RCSB], [https://www.ebi.ac.uk/pdbsum/1uhb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1uhb ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TRYP_PIG TRYP_PIG]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/uh/1uhb_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1uhb ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Trypsin, a serine protease enzyme plays a pivotal role in digestion and is autocatalytic. The crystal structure of a complex formed between porcine trypsin and an auto catalytically produced peptide is reported here. This complex shows a reduction in enzyme activity as compared to native beta-trypsin. The nonapeptide has a lysine, which is recognized by Asp 189 at the specificity pocket. The auto catalytically produced native nonapeptide is bound at the active site cleft like other trypsin inhibitors but the important interactions with the oxyanion hole are absent. The peptide covers only a part of the active site cleft and hence the enzyme activity is reduced rather than being inhibited.
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==About this Structure==
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Trypsin activity reduced by an autocatalytically produced nonapeptide.,Ibrahim BS, Shamaladevi N, Pattabhi V J Biomol Struct Dyn. 2004 Jun;21(6):737-44. PMID:15106996<ref>PMID:15106996</ref>
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1UHB is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with ACT and CA as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UHB OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Trypsin activity reduced by an autocatalytically produced nonapeptide., Ibrahim BS, Shamaladevi N, Pattabhi V, J Biomol Struct Dyn. 2004 Jun;21(6):737-44. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15106996 15106996]
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</div>
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[[Category: Protein complex]]
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<div class="pdbe-citations 1uhb" style="background-color:#fffaf0;"></div>
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[[Category: Sus scrofa]]
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[[Category: Trypsin]]
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[[Category: Ibrahim, B.Syed.]]
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[[Category: Pattabhi, V.]]
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[[Category: Shamaladevi, N.]]
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[[Category: ACT]]
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[[Category: CA]]
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[[Category: hydrolase]]
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[[Category: peptide trypsin complex]]
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[[Category: serine protease]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 04:07:34 2007''
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==See Also==
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*[[Trypsin 3D structures|Trypsin 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Sus scrofa]]
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[[Category: Pattabhi V]]
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[[Category: Shamaladevi N]]
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[[Category: Syed Ibrahim B]]

Current revision

Crystal structure of porcine alpha trypsin bound with auto catalyticaly produced native peptide at 2.15 A resolution

PDB ID 1uhb

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