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1uis

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(New page: 200px<br /><applet load="1uis" size="450" color="white" frame="true" align="right" spinBox="true" caption="1uis, resolution 2.0&Aring;" /> '''The 2.0 crystal struc...)
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[[Image:1uis.gif|left|200px]]<br /><applet load="1uis" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1uis, resolution 2.0&Aring;" />
 
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'''The 2.0 crystal structure of eqFP611, a far-red fluorescent protein from the sea anemone Entacmaea quadricolor'''<br />
 
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==Overview==
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==The 2.0 crystal structure of eqFP611, a far-red fluorescent protein from the sea anemone Entacmaea quadricolor==
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We have crystallized and subsequently determined to 2.0-A resolution the, crystal structure of eqFP611, a far red fluorescent protein from the sea, anemone Entacmaea quadricolor. The structure of the protomer, which adopts, a beta-can topology, is similar to that of the related monomeric green, fluorescent protein (GFP). The quaternary structure of eqFP611, a tetramer, exhibiting 222 symmetry, is similar to that observed for the more closely, related red fluorescent protein DsRed and the chromoprotein Rtms5. The, unique chromophore sequence (Met63-Tyr64-Gly65) of eqFP611, adopts a, coplanar and trans conformation within the interior of the beta-can fold., Accordingly, the eqFP611 chromophore adopts a significantly different, conformation in comparison to the chromophore conformation observed in, GFP, DsRed, and Rtms5. The coplanar chromophore conformation and its, immediate environment provide a structural basis for the far red, highly, fluorescent nature of eqFP611. The eqFP611 structure extends our knowledge, on the range of conformations a chromophore can adopt within closely, related members of the green fluorescent protein family.
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<StructureSection load='1uis' size='340' side='right'caption='[[1uis]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1uis]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Entacmaea_quadricolor Entacmaea quadricolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UIS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UIS FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NRQ:{(4Z)-4-(4-HYDROXYBENZYLIDENE)-2-[3-(METHYLTHIO)PROPANIMIDOYL]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>NRQ</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1uis FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uis OCA], [https://pdbe.org/1uis PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1uis RCSB], [https://www.ebi.ac.uk/pdbsum/1uis PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1uis ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RFP_ENTQU RFP_ENTQU] Pigment protein.<ref>PMID:12185250</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ui/1uis_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1uis ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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We have crystallized and subsequently determined to 2.0-A resolution the crystal structure of eqFP611, a far red fluorescent protein from the sea anemone Entacmaea quadricolor. The structure of the protomer, which adopts a beta-can topology, is similar to that of the related monomeric green fluorescent protein (GFP). The quaternary structure of eqFP611, a tetramer exhibiting 222 symmetry, is similar to that observed for the more closely related red fluorescent protein DsRed and the chromoprotein Rtms5. The unique chromophore sequence (Met63-Tyr64-Gly65) of eqFP611, adopts a coplanar and trans conformation within the interior of the beta-can fold. Accordingly, the eqFP611 chromophore adopts a significantly different conformation in comparison to the chromophore conformation observed in GFP, DsRed, and Rtms5. The coplanar chromophore conformation and its immediate environment provide a structural basis for the far red, highly fluorescent nature of eqFP611. The eqFP611 structure extends our knowledge on the range of conformations a chromophore can adopt within closely related members of the green fluorescent protein family.
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==About this Structure==
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The 2.0-A crystal structure of eqFP611, a far red fluorescent protein from the sea anemone Entacmaea quadricolor.,Petersen J, Wilmann PG, Beddoe T, Oakley AJ, Devenish RJ, Prescott M, Rossjohn J J Biol Chem. 2003 Nov 7;278(45):44626-31. Epub 2003 Aug 8. PMID:12909624<ref>PMID:12909624</ref>
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1UIS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Entacmaea_quadricolor Entacmaea quadricolor] with CA and ACY as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UIS OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The 2.0-A crystal structure of eqFP611, a far red fluorescent protein from the sea anemone Entacmaea quadricolor., Petersen J, Wilmann PG, Beddoe T, Oakley AJ, Devenish RJ, Prescott M, Rossjohn J, J Biol Chem. 2003 Nov 7;278(45):44626-31. Epub 2003 Aug 8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12909624 12909624]
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</div>
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[[Category: Entacmaea quadricolor]]
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<div class="pdbe-citations 1uis" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Beddoe, T.]]
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[[Category: Devenish, R.J.]]
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[[Category: Oakley, A.J.]]
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[[Category: Petersen, J.]]
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[[Category: Prescott, M.]]
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[[Category: Rossjohn, J.]]
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[[Category: Wilmann, P.G.]]
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[[Category: ACY]]
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[[Category: CA]]
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[[Category: luminescent protein]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 04:09:46 2007''
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==See Also==
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*[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Entacmaea quadricolor]]
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[[Category: Large Structures]]
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[[Category: Beddoe T]]
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[[Category: Devenish RJ]]
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[[Category: Oakley AJ]]
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[[Category: Petersen J]]
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[[Category: Prescott M]]
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[[Category: Rossjohn J]]
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[[Category: Wilmann PG]]

Current revision

The 2.0 crystal structure of eqFP611, a far-red fluorescent protein from the sea anemone Entacmaea quadricolor

PDB ID 1uis

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