3cpr

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{{Seed}}
 
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[[Image:3cpr.jpg|left|200px]]
 
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==The crystal structure of Corynebacterium glutamicum dihydrodipicolinate synthase to 2.2 A resolution==
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The line below this paragraph, containing "STRUCTURE_3cpr", creates the "Structure Box" on the page.
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<StructureSection load='3cpr' size='340' side='right'caption='[[3cpr]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3cpr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Corynebacterium_glutamicum Corynebacterium glutamicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CPR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CPR FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=KPI:(2S)-2-AMINO-6-[(1-HYDROXY-1-OXO-PROPAN-2-YLIDENE)AMINO]HEXANOIC+ACID'>KPI</scene></td></tr>
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{{STRUCTURE_3cpr| PDB=3cpr | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3cpr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cpr OCA], [https://pdbe.org/3cpr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3cpr RCSB], [https://www.ebi.ac.uk/pdbsum/3cpr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3cpr ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DAPA_CORGL DAPA_CORGL] Catalyzes the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to 4-hydroxy-tetrahydrodipicolinate (HTPA).[HAMAP-Rule:MF_00418]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cp/3cpr_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3cpr ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The lysine insensitive Corynebacterium glutamicum dihydrodipicolinate synthase enzyme (cDHDPS) was recently successfully introduced into maize plants to enhance the level of lysine in the grain. To better understand lysine insensitivity of the cDHDPS, we expressed, purified, kinetically characterized the protein, and solved its X-ray crystal structure. The cDHDPS enzyme has a fold and overall structure that is highly similar to other DHDPS proteins. A noteworthy feature of the active site is the evidence that the catalytic lysine residue forms a Schiff base adduct with pyruvate. Analyses of the cDHDPS structure in the vicinity of the putative binding site for S-lysine revealed that the allosteric binding site in the Escherichia coli DHDPS protein does not exist in cDHDPS due to three non-conservative amino acids substitutions, and this is likely why cDHDPS is not feedback inhibited by lysine.
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===The crystal structure of Corynebacterium glutamicum dihydrodipicolinate synthase to 2.2 A resolution===
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Characterization and crystal structure of lysine insensitive Corynebacterium glutamicum dihydrodipicolinate synthase (cDHDPS) protein.,Rice EA, Bannon GA, Glenn KC, Jeong SS, Sturman EJ, Rydel TJ Arch Biochem Biophys. 2008 Dec 15;480(2):111-21. Epub 2008 Oct 7. PMID:18930704<ref>PMID:18930704</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3cpr" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_18930704}}, adds the Publication Abstract to the page
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*[[Dihydrodipicolinate synthase|Dihydrodipicolinate synthase]]
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(as it appears on PubMed at http://www.pubmed.gov), where 18930704 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_18930704}}
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__TOC__
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</StructureSection>
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==About this Structure==
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3CPR is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Corynebacterium_glutamicum Corynebacterium glutamicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CPR OCA].
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==Reference==
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Characterization and crystal structure of lysine insensitive Corynebacterium glutamicum dihydrodipicolinate synthase (cDHDPS) protein., Rice EA, Bannon GA, Glenn KC, Jeong SS, Sturman EJ, Rydel TJ, Arch Biochem Biophys. 2008 Dec 15;480(2):111-21. Epub 2008 Oct 7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18930704 18930704]
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[[Category: Corynebacterium glutamicum]]
[[Category: Corynebacterium glutamicum]]
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[[Category: Dihydrodipicolinate synthase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Rice EA]]
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[[Category: Rice, E A.]]
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[[Category: Rydel TJ]]
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[[Category: Rydel, T J.]]
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[[Category: Sturman EJ]]
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[[Category: Sturman, E J.]]
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[[Category: Amino-acid biosynthesis]]
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[[Category: Cytoplasm]]
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[[Category: Diaminopimelate biosynthesis]]
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[[Category: Lyase]]
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[[Category: Lysine biosynthesis]]
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[[Category: Schiff base]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Nov 26 20:05:52 2008''
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Current revision

The crystal structure of Corynebacterium glutamicum dihydrodipicolinate synthase to 2.2 A resolution

PDB ID 3cpr

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