3cz4

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{{Seed}}
 
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[[Image:3cz4.png|left|200px]]
 
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==Native AphA class B acid phosphatase/phosphotransferase from E. coli==
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The line below this paragraph, containing "STRUCTURE_3cz4", creates the "Structure Box" on the page.
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<StructureSection load='3cz4' size='340' side='right'caption='[[3cz4]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3cz4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CZ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CZ4 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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{{STRUCTURE_3cz4| PDB=3cz4 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3cz4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cz4 OCA], [https://pdbe.org/3cz4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3cz4 RCSB], [https://www.ebi.ac.uk/pdbsum/3cz4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3cz4 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/APHA_ECOLI APHA_ECOLI] Dephosphorylates several organic phosphate monoesters including 3'- and 5'-nucleotides, 2'-deoxy-5'-nucleotides, pNPP, phenyl phosphate, glycerol 2-phosphate, ribose 5-phosphate, O-phospho-L-amino acids and phytic acid, showing the highest activity with aryl phosphoesters (pNPP, phenyl phosphate and O-phospho-L-tyrosine), and to a lesser extent with 3'- and 5'-nucleotides. No activity toward ATP, phosphodiesters, glycerol-1-phosphate, glucose 1-phosphate, glucose 6-phosphate, NADP, GTP or 3',5'-cAMP, ADP or ATP. Also has a phosphotransferase activity catalyzing the transfer of low-energy phosphate groups from organic phosphate monoesters to free hydroxyl groups of various organic compounds. Capable of transferring phosphate from either pNPP or UMP to adenosine or uridine. Does not exhibit nucleotide phosphomutase activity.<ref>PMID:9011040</ref> <ref>PMID:16297670</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cz/3cz4_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3cz4 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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AphA is a magnesium-dependent, bacterial class B acid phosphatase that catalyzes the hydrolysis of a variety of phosphoester substrates and belongs to the DDDD superfamily of phosphohydrolases. The recently reported crystal structure of AphA from Escherichia coli has revealed the quaternary structure of the enzyme together with hints about its catalytic mechanism. The present work reports the crystal structures of AphA from E. coli in complex with substrate, transition-state, and intermediate analogues. The structures provide new insights into the mechanism of the enzyme and allow a revision of some aspects of the previously proposed mechanism that have broader implications for all the phosphatases of the DDDD superfamily.
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===Native AphA class B acid phosphatase/phosphotransferase from E. coli===
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Structural insights into the catalytic mechanism of the bacterial class B phosphatase AphA belonging to the DDDD superfamily of phosphohydrolases.,Leone R, Cappelletti E, Benvenuti M, Lentini G, Thaller MC, Mangani S J Mol Biol. 2008 Dec 12;384(2):478-88. Epub 2008 Sep 27. PMID:18845157<ref>PMID:18845157</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3cz4" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_18845157}}, adds the Publication Abstract to the page
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*[[Acid phosphatase 3D structures|Acid phosphatase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 18845157 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_18845157}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Escherichia coli K-12]]
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3CZ4 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CZ4 OCA].
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[[Category: Large Structures]]
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[[Category: Benvenuti M]]
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==Reference==
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[[Category: Cappelletti E]]
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Structural insights into the catalytic mechanism of the bacterial class B phosphatase AphA belonging to the DDDD superfamily of phosphohydrolases., Leone R, Cappelletti E, Benvenuti M, Lentini G, Thaller MC, Mangani S, J Mol Biol. 2008 Dec 12;384(2):478-88. Epub 2008 Sep 27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18845157 18845157]
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[[Category: Lentini G]]
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[[Category: Leone R]]
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A structure-based proposal for the catalytic mechanism of the bacterial acid phosphatase AphA belonging to the DDDD superfamily of phosphohydrolases., Calderone V, Forleo C, Benvenuti M, Thaller MC, Rossolini GM, Mangani S, J Mol Biol. 2006 Jan 27;355(4):708-21. Epub 2005 Nov 10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16330049 16330049]
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[[Category: Mangani S]]
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[[Category: Acid phosphatase]]
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[[Category: Thaller MC]]
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[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Benvenuti, M.]]
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[[Category: Cappelletti, E.]]
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[[Category: Lentini, G.]]
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[[Category: Leone, R.]]
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[[Category: Mangani, S.]]
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[[Category: Thaller, M C.]]
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[[Category: Acid phosphatase/phosphotransferase]]
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[[Category: Hydrolase]]
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[[Category: Magnesium]]
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[[Category: Metal-binding]]
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[[Category: Metallo phosphatase]]
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[[Category: Periplasm]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Nov 26 20:10:26 2008''
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Current revision

Native AphA class B acid phosphatase/phosphotransferase from E. coli

PDB ID 3cz4

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