2w11

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{{Seed}}
 
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[[Image:2w11.jpg|left|200px]]
 
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==Structure of the L-2-haloacid dehalogenase from Sulfolobus tokodaii==
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The line below this paragraph, containing "STRUCTURE_2w11", creates the "Structure Box" on the page.
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<StructureSection load='2w11' size='340' side='right'caption='[[2w11]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2w11]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Sulfurisphaera_tokodaii Sulfurisphaera tokodaii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W11 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2W11 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2OP:(2S)-2-HYDROXYPROPANOIC+ACID'>2OP</scene></td></tr>
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{{STRUCTURE_2w11| PDB=2w11 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2w11 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w11 OCA], [https://pdbe.org/2w11 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2w11 RCSB], [https://www.ebi.ac.uk/pdbsum/2w11 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2w11 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q96XE7_SULTO Q96XE7_SULTO]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Haloacid dehalogenases have potential applications in the pharmaceutical and fine chemical industry as well as in the remediation of contaminated land. The L: -2-haloacid dehalogenase from the thermophilic archaeon Sulfolobus tokodaii has been cloned and over-expressed in Escherichia coli and successfully purified to homogeneity. Here we report the structure of the recombinant dehalogenase solved by molecular replacement in two different crystal forms. The enzyme is a homodimer with each monomer being composed of a core-domain of a beta-sheet bundle surrounded by alpha-helices and an alpha-helical sub-domain. This fold is similar to previously solved mesophilic L: -haloacid dehalogenase structures. The monoclinic crystal form contains a putative inhibitor L: -lactate in the active site. The enzyme displays haloacid dehalogenase activity towards carboxylic acids with the halide attached at the C2 position with the highest activity towards chloropropionic acid. The enzyme is thermostable with maximum activity at 60 degrees C and a half-life of over 1 h at 70 degrees C. The enzyme is relatively stable to solvents with 25% activity lost when incubated for 1 h in 20% v/v DMSO.
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===STRUCTURE OF THE L-2-HALOACID DEHALOGENASE FROM SULFOLOBUS TOKODAII===
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Biochemical and structural studies of a L: -haloacid dehalogenase from the thermophilic archaeon Sulfolobus tokodaii.,Rye CA, Isupov MN, Lebedev AA, Littlechild JA Extremophiles. 2008 Nov 29. PMID:19039518<ref>PMID:19039518</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2w11" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[Dehalogenase 3D structures|Dehalogenase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 19039518 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19039518}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2W11 is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Sulfolobus_tokodaii Sulfolobus tokodaii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W11 OCA].
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[[Category: Sulfurisphaera tokodaii]]
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[[Category: Isupov MN]]
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==Reference==
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[[Category: Lebedev AA]]
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Biochemical and structural studies of a L: -haloacid dehalogenase from the thermophilic archaeon Sulfolobus tokodaii., Rye CA, Isupov MN, Lebedev AA, Littlechild JA, Extremophiles. 2008 Nov 29. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/19039518 19039518]
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[[Category: Littlechild JA]]
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[[Category: Rye CA]]
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An order-disorder twin crystal of L-2-haloacid dehalogenase from Sulfolobus tokodaii., Rye CA, Isupov MN, Lebedev AA, Littlechild JA, Acta Crystallogr D Biol Crystallogr. 2007 Aug;63(Pt 8):926-30. Epub 2007, Jul 17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17642519 17642519]
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[[Category: Sulfolobus tokodaii]]
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[[Category: Isupov, M N.]]
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[[Category: Lebedev, A A.]]
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[[Category: Littlechild, J A.]]
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[[Category: Rye, C A.]]
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[[Category: Had]]
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[[Category: Haloacid dehalogenase]]
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[[Category: Hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 10 14:55:08 2008''
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Current revision

Structure of the L-2-haloacid dehalogenase from Sulfolobus tokodaii

PDB ID 2w11

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