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2ahv

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{{Seed}}
 
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[[Image:2ahv.png|left|200px]]
 
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==Crystal Structure of Acyl-CoA transferase from E. coli O157:H7 (YdiF)-thioester complex with CoA- 1==
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The line below this paragraph, containing "STRUCTURE_2ahv", creates the "Structure Box" on the page.
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<StructureSection load='2ahv' size='340' side='right'caption='[[2ahv]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2ahv]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7 Escherichia coli O157:H7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AHV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AHV FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene></td></tr>
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{{STRUCTURE_2ahv| PDB=2ahv | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ahv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ahv OCA], [https://pdbe.org/2ahv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ahv RCSB], [https://www.ebi.ac.uk/pdbsum/2ahv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ahv ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/YDIF_ECO57 YDIF_ECO57] CoA transferase having broad substrate specificity for short-chain acyl-CoA thioesters with the activity decreasing when the length of the carboxylic acid chain exceeds four carbons. Exhibits high activity with acetoacetyl-CoA, propionyl-CoA, crotonoyl-CoA or butyryl-CoA as donors, with acetate as an acceptor. When acetyl-CoA is used as the donor, propionate, acetoacetate, butyrate, isobutyrate, and 4-hydroxybutyrate can be utilized as acceptors but not isovalerate. May play a role in short-chain fatty acid metabolism in E.coli.<ref>PMID:16253988</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ah/2ahv_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ahv ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Coenzyme A transferases are involved in a broad range of biochemical processes in both prokaryotes and eukaryotes, and exhibit a diverse range of substrate specificities. The YdiF protein from Escherichia coli O157:H7 is an acyl-CoA transferase of unknown physiological function, and belongs to a large sequence family of CoA transferases, present in bacteria to humans, which utilize oxoacids as acceptors. In vitro measurements showed that YdiF displays enzymatic activity with short-chain acyl-CoAs. The crystal structures of YdiF and its complex with CoA, the first co-crystal structure for any Family I CoA transferase, have been determined and refined at 1.9 and 2.0 A resolution, respectively. YdiF is organized into tetramers, with each monomer having an open alpha/beta structure characteristic of Family I CoA transferases. Co-crystallization of YdiF with a variety of CoA thioesters in the absence of acceptor carboxylic acid resulted in trapping a covalent gamma-glutamyl-CoA thioester intermediate. The CoA binds within a well defined pocket at the N- and C-terminal domain interface, but makes contact only with the C-terminal domain. The structure of the YdiF complex provides a basis for understanding the different catalytic steps in the reaction of Family I CoA transferases.
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===Crystal Structure of Acyl-CoA transferase from E. coli O157:H7 (YdiF)-thioester complex with CoA- 1===
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Crystallographic trapping of the glutamyl-CoA thioester intermediate of family I CoA transferases.,Rangarajan ES, Li Y, Ajamian E, Iannuzzi P, Kernaghan SD, Fraser ME, Cygler M, Matte A J Biol Chem. 2005 Dec 30;280(52):42919-28. Epub 2005 Oct 27. PMID:16253988<ref>PMID:16253988</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_16253988}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2ahv" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 16253988 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_16253988}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Escherichia coli O157:H7]]
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2AHV is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli_o157:h7 Escherichia coli o157:h7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AHV OCA].
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[[Category: Large Structures]]
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[[Category: Ajamian E]]
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==Reference==
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[[Category: Cygler M]]
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Crystallographic trapping of the glutamyl-CoA thioester intermediate of family I CoA transferases., Rangarajan ES, Li Y, Ajamian E, Iannuzzi P, Kernaghan SD, Fraser ME, Cygler M, Matte A, J Biol Chem. 2005 Dec 30;280(52):42919-28. Epub 2005 Oct 27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16253988 16253988]
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[[Category: Fraser ME]]
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[[Category: Escherichia coli o157:h7]]
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[[Category: Iannuzzi P]]
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[[Category: Ajamian, E.]]
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[[Category: Kernaghan SD]]
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[[Category: BSGI, Montreal-Kingston Bacterial Structural Genomics Initiative.]]
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[[Category: Li Y]]
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[[Category: Cygler, M.]]
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[[Category: Matte A]]
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[[Category: Fraser, M E.]]
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[[Category: Rangarajan ES]]
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[[Category: Iannuzzi, P.]]
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[[Category: Kernaghan, S D.]]
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[[Category: Li, Y.]]
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[[Category: Matte, A.]]
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[[Category: Rangarajan, E S.]]
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[[Category: Bsgi]]
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[[Category: Coa transferase]]
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[[Category: Glutamyl thioester]]
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[[Category: Montreal-kingston bacterial structural genomics initiative]]
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[[Category: Structural genomic]]
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[[Category: Ydif]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 10 15:13:29 2008''
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Current revision

Crystal Structure of Acyl-CoA transferase from E. coli O157:H7 (YdiF)-thioester complex with CoA- 1

PDB ID 2ahv

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