This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.




2vvs

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (15:34, 13 December 2023) (edit) (undo)
 
(11 intermediate revisions not shown.)
Line 1: Line 1:
-
{{Seed}}
 
-
[[Image:2vvs.png|left|200px]]
 
-
<!--
+
==BtGH84 structure in complex with PUGNAc==
-
The line below this paragraph, containing "STRUCTURE_2vvs", creates the "Structure Box" on the page.
+
<StructureSection load='2vvs' size='340' side='right'caption='[[2vvs]], [[Resolution|resolution]] 2.24&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[2vvs]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacteroides_thetaiotaomicron_VPI-5482 Bacteroides thetaiotaomicron VPI-5482]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VVS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VVS FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.24&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=OAN:O-(2-ACETAMIDO-2-DEOXY+D-GLUCOPYRANOSYLIDENE)+AMINO-N-PHENYLCARBAMATE'>OAN</scene></td></tr>
-
{{STRUCTURE_2vvs| PDB=2vvs | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vvs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vvs OCA], [https://pdbe.org/2vvs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vvs RCSB], [https://www.ebi.ac.uk/pdbsum/2vvs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vvs ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/OGA_BACTN OGA_BACTN] Biological function unknown. Capable of hydrolyzing the glycosidic link of O-GlcNAcylated proteins.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vv/2vvs_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2vvs ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The O-GlcNAc post-translational modification is considered to act as a sensor of nutrient flux through the hexosamine biosynthetic pathway. A cornerstone of this hypothesis is that global elevation of protein O-GlcNAc levels, typically induced with the non-selective O-GlcNAcase inhibitor PUGNAc (O-(2-acetamido-2-deoxy-D-glycopyranosylidene) amino-N-phenylcarbamate), causes insulin resistance in adipocytes. Here we address the potential link between elevated O-GlcNAc and insulin resistance by using a potent and selective inhibitor of O-GlcNAcase (NButGT (1,2-dideoxy-2'-propyl-alpha-D-glucopyranoso-[2,1-D]-Delta 2'-thiazoline), 1200-fold selectivity). A comparison of the structures of a bacterial homologue of O-GlcNAcase in complex with PUGNAc or NButGT reveals that these inhibitors bind to the same region of the active site, underscoring the competitive nature of their inhibition of O-GlcNAcase and the molecular basis of selectivity. Treating 3T3-L1 adipocytes with NButGT induces rapid increases in global O-GlcNAc levels, but strikingly, NButGT treatment does not replicate the insulin desensitizing effects of the non-selective O-GlcNAcase inhibitor PUGNAc. Consistent with these observations, NButGT also does not recapitulate the impaired insulin-mediated phosphorylation of Akt that is induced by treatment with PUGNAc. Collectively, these results suggest that increases in global levels of O-GlcNAc-modified proteins of cultured adipocytes do not, on their own, cause insulin resistance.
-
===BTGH84 STRUCTURE IN COMPLEX WITH PUGNAC===
+
Elevation of global O-GlcNAc levels in 3T3-L1 adipocytes by selective inhibition of O-GlcNAcase does not induce insulin resistance.,Macauley MS, Bubb AK, Martinez-Fleites C, Davies GJ, Vocadlo DJ J Biol Chem. 2008 Dec 12;283(50):34687-95. Epub 2008 Oct 8. PMID:18842583<ref>PMID:18842583</ref>
 +
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 2vvs" style="background-color:#fffaf0;"></div>
-
<!--
+
==See Also==
-
The line below this paragraph, {{ABSTRACT_PUBMED_18842583}}, adds the Publication Abstract to the page
+
*[[Beta-Hexosaminidase|Beta-Hexosaminidase]]
-
(as it appears on PubMed at http://www.pubmed.gov), where 18842583 is the PubMed ID number.
+
*[[Beta-Hexosaminidase 3D structures|Beta-Hexosaminidase 3D structures]]
-
-->
+
*[[O-GlcNAcase|O-GlcNAcase]]
-
{{ABSTRACT_PUBMED_18842583}}
+
== References ==
-
 
+
<references/>
-
==About this Structure==
+
__TOC__
-
2VVS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacteria Bacteria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VVS OCA].
+
</StructureSection>
-
 
+
[[Category: Bacteroides thetaiotaomicron VPI-5482]]
-
==Reference==
+
[[Category: Large Structures]]
-
Elevation of Global O-GlcNAc Levels in 3T3-L1 Adipocytes by Selective Inhibition of O-GlcNAcase Does Not Induce Insulin Resistance., Macauley MS, Bubb AK, Martinez-Fleites C, Davies GJ, Vocadlo DJ, J Biol Chem. 2008 Dec 12;283(50):34687-95. Epub 2008 Oct 8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18842583 18842583]
+
[[Category: Bubb A]]
-
[[Category: Bacteria]]
+
[[Category: Davies GJ]]
-
[[Category: Beta-N-acetylhexosaminidase]]
+
[[Category: Macauley MS]]
-
[[Category: Single protein]]
+
[[Category: Martinez-Fleites C]]
-
[[Category: Pdbx_ordinal=, <PDBx:audit_author.]]
+
[[Category: Vocadlo DJ]]
-
[[Category: Glycoside hydrolase]]
+
-
[[Category: Hydrolase]]
+
-
[[Category: Inhibitor]]
+
-
[[Category: O-glcnac]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 17 20:09:45 2008''
+

Current revision

BtGH84 structure in complex with PUGNAc

PDB ID 2vvs

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2vvs"
Personal tools