3bdn

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{{Seed}}
 
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[[Image:3bdn.png|left|200px]]
 
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==Crystal Structure of the Lambda Repressor==
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The line below this paragraph, containing "STRUCTURE_3bdn", creates the "Structure Box" on the page.
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<StructureSection load='3bdn' size='340' side='right'caption='[[3bdn]], [[Resolution|resolution]] 3.91&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3bdn]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_Lambda Escherichia virus Lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BDN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BDN FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.909&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bdn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bdn OCA], [https://pdbe.org/3bdn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bdn RCSB], [https://www.ebi.ac.uk/pdbsum/3bdn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bdn ProSAT]</span></td></tr>
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{{STRUCTURE_3bdn| PDB=3bdn | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RPC1_LAMBD RPC1_LAMBD] Repressor protein CI allows phage lambda to reside inactively in the chromosome of its host bacterium. This lysogenic state is maintained by binding of regulatory protein CI to the OR and OL operators, preventing transcription of proteins necessary for lytic development.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bd/3bdn_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3bdn ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Bacteriophage lambda has for many years been a model system for understanding mechanisms of gene regulation. A 'genetic switch' enables the phage to transition from lysogenic growth to lytic development when triggered by specific environmental conditions. The key component of the switch is the cI repressor, which binds to two sets of three operator sites on the lambda chromosome that are separated by about 2,400 base pairs (bp). A hallmark of the lambda system is the pairwise cooperativity of repressor binding. In the absence of detailed structural information, it has been difficult to understand fully how repressor molecules establish the cooperativity complex. Here we present the X-ray crystal structure of the intact lambda cI repressor dimer bound to a DNA operator site. The structure of the repressor, determined by multiple isomorphous replacement methods, reveals an unusual overall architecture that allows it to adopt a conformation that appears to facilitate pairwise cooperative binding to adjacent operator sites.
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===Crystal Structure of the Lambda Repressor===
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Crystal structure of the lambda repressor and a model for pairwise cooperative operator binding.,Stayrook S, Jaru-Ampornpan P, Ni J, Hochschild A, Lewis M Nature. 2008 Apr 24;452(7190):1022-5. PMID:18432246<ref>PMID:18432246</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_18432246}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3bdn" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 18432246 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_18432246}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Escherichia virus Lambda]]
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3BDN is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BDN OCA].
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[[Category: Large Structures]]
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[[Category: Hochschild A]]
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==Reference==
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[[Category: Jaru-Ampornpan P]]
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Crystal structure of the lambda repressor and a model for pairwise cooperative operator binding., Stayrook S, Jaru-Ampornpan P, Ni J, Hochschild A, Lewis M, Nature. 2008 Apr 24;452(7190):1022-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18432246 18432246]
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[[Category: Lewis M]]
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[[Category: Enterobacteria phage lambda]]
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[[Category: Stayrook SE]]
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[[Category: Hochschild, A.]]
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[[Category: Jaru-Ampornpan, P.]]
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[[Category: Lewis, M.]]
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[[Category: Stayrook, S E.]]
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[[Category: Allostery]]
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[[Category: Cooperativity]]
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[[Category: Dna binding]]
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[[Category: Lambda]]
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[[Category: Repressor]]
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[[Category: Transcription/dna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 24 11:03:34 2008''
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Current revision

Crystal Structure of the Lambda Repressor

PDB ID 3bdn

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