1vj7

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(New page: 200px<br /><applet load="1vj7" size="450" color="white" frame="true" align="right" spinBox="true" caption="1vj7, resolution 2.10&Aring;" /> '''Crystal structure of...)
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[[Image:1vj7.gif|left|200px]]<br /><applet load="1vj7" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1vj7, resolution 2.10&Aring;" />
 
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'''Crystal structure of the bifunctional catalytic fragment of RelSeq, the RelA/SpoT homolog from Streptococcus equisimilis.'''<br />
 
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==Overview==
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==Crystal structure of the bifunctional catalytic fragment of RelSeq, the RelA/SpoT homolog from Streptococcus equisimilis.==
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Enzymes of the Rel/Spo family enable bacteria to survive prolonged periods, of nutrient limitation by producing an intracellular signaling alarmone, (p)ppGpp, which triggers the so-called stringent response. Both the, synthesis of (p)ppGpp from ATP and GDP(GTP), and its hydrolysis to, GDP(GTP) and pyrophosphate, are catalyzed by Rel/Spo proteins. The 2.1 A, crystal structure of the bifunctional catalytic fragment of the Rel/Spo, homolog from Streptococcus dysgalactiae subsp. equisimilis, Rel(Seq), reveals two conformations of the enzyme corresponding to known reciprocal, activity states: (p)ppGpp-hydrolase-OFF/(p)ppGpp-synthetase-ON and, hydrolase-ON/synthetase-OFF. The hydrolase and synthetase domains bear, remarkable similarities to the catalytic domains of the cyclic, phosphodiesterase and nucleotidyltransferase superfamilies, respectively., The active sites, separated by more than 30 A, contain bound nucleotides, including an unusual (p)ppGpp derivative, GDP-2':3'-cyclic monophosphate., Reciprocal regulation of the antagonistic catalytic activities, suggested, by the structure, is supported by mutagenesis experiments and appears to, involve ligand-induced signal transmission between the two active sites.
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<StructureSection load='1vj7' size='340' side='right'caption='[[1vj7]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1vj7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_dysgalactiae_subsp._equisimilis Streptococcus dysgalactiae subsp. equisimilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VJ7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1VJ7 FirstGlance]. <br>
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1VJ7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_dysgalactiae_subsp._equisimilis Streptococcus dysgalactiae subsp. equisimilis] with MN, GDP and GPX as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1VJ7 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GPX:GUANOSINE+5-DIPHOSPHATE+2 3-CYCLIC+MONOPHOSPHATE'>GPX</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1vj7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vj7 OCA], [https://pdbe.org/1vj7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1vj7 RCSB], [https://www.ebi.ac.uk/pdbsum/1vj7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1vj7 ProSAT]</span></td></tr>
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Conformational antagonism between opposing active sites in a bifunctional RelA/SpoT homolog modulates (p)ppGpp metabolism during the stringent response [corrected]., Hogg T, Mechold U, Malke H, Cashel M, Hilgenfeld R, Cell. 2004 Apr 2;117(1):57-68. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15066282 15066282]
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</table>
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[[Category: Single protein]]
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== Function ==
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[https://www.uniprot.org/uniprot/RELA_STREQ RELA_STREQ] In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes both the formation of pppGpp which is then hydrolyzed to form ppGpp, and the hydrolysis of ppGpp. The enzyme does not similtaneously display both synthase and hydrolase activities. In the structure of residues 1-385 there are 2 conformations seen, the hydrolase-OFF/synthase-ON and hydrolase-ON/synthase-OFF, suggesting there is ligand-induced signal transmission between the 2 active sites.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vj/1vj7_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1vj7 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Streptococcus dysgalactiae subsp. equisimilis]]
[[Category: Streptococcus dysgalactiae subsp. equisimilis]]
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[[Category: Cashel, M.]]
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[[Category: Cashel M]]
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[[Category: Hilgenfeld, R.]]
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[[Category: Hilgenfeld R]]
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[[Category: Hogg, T.]]
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[[Category: Hogg T]]
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[[Category: Malke, H.]]
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[[Category: Malke H]]
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[[Category: Mechold, U.]]
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[[Category: Mechold U]]
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[[Category: GDP]]
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[[Category: GPX]]
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[[Category: MN]]
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[[Category: (p)ppgpp]]
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[[Category: alpha beta 2-layer sandwich]]
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[[Category: gdp]]
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[[Category: hd domain]]
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[[Category: helix bundle]]
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[[Category: magic spot]]
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[[Category: manganese]]
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[[Category: ppg2':3'p]]
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[[Category: ppgpp]]
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[[Category: rela]]
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[[Category: rsh]]
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[[Category: spot]]
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[[Category: stringent effector]]
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[[Category: stringent factor]]
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[[Category: stringent response]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 04:54:05 2007''
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Current revision

Crystal structure of the bifunctional catalytic fragment of RelSeq, the RelA/SpoT homolog from Streptococcus equisimilis.

PDB ID 1vj7

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