3drw

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{{Seed}}
 
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[[Image:3drw.jpg|left|200px]]
 
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==Crystal Structure of a Phosphofructokinase from Pyrococcus horikoshii OT3 with AMP==
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The line below this paragraph, containing "STRUCTURE_3drw", creates the "Structure Box" on the page.
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<StructureSection load='3drw' size='340' side='right'caption='[[3drw]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3drw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DRW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3DRW FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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{{STRUCTURE_3drw| PDB=3drw | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3drw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3drw OCA], [https://pdbe.org/3drw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3drw RCSB], [https://www.ebi.ac.uk/pdbsum/3drw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3drw ProSAT], [https://www.topsan.org/Proteins/MCSG/3drw TOPSAN]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/K6PF_PYRHO K6PF_PYRHO] Catalyzes the phosphorylation of fructose 6-phosphate to fructose 1,6-bisphosphate using ADP as the phosphate donor (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dr/3drw_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3drw ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Some hyperthermophilic archaea use a modified glycolytic pathway that employs an ADP-dependent glucokinase (ADP-GK) and an ADP-dependent phosphofructokinase (ADP-PFK) or, in the case of Methanococcus jannaschii, a bifunctional ADP-dependent glucophosphofructokinase (ADP-GK/PFK). The crystal structures of three ADP-GKs have been determined. However, there is no structural information available for ADP-PFKs or the ADP-GK/PFK. Here, we present the first crystal structure of an ADP-PFK from Pyrococcus horikoshii OT3 (PhPFK) in both apo- and AMP-bound forms determined to 2.0-A and 1.9-A resolution, respectively, along with biochemical characterization of the enzyme. The overall structure of PhPFK maintains a similar large and small alpha/beta domain structure seen in the ADP-GK structures. A large conformational change accompanies binding of phosphoryl donor, acceptor, or both, in all members of the ribokinase superfamily characterized thus far, which is believed to be critical to enzyme function. Surprisingly, no such conformational change was observed in the AMP-bound PhPFK structure compared with the apo structure. Through comprehensive site-directed mutagenesis of the substrate binding pocket we identified residues that were critical for both substrate recognition and the phosphotransfer reaction. The catalytic residues and many of the substrate binding residues are conserved between PhPFK and ADP-GKs; however, four key residues differ in the sugar-binding pocket, which we have shown determine the sugar-binding specificity. Using these results we were able to engineer a mutant PhPFK that mimics the ADP-GK/PFK and is able to phosphorylate both fructose 6-phosphate and glucose.
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===Crystal Structure of a Phosphofructokinase from Pyrococcus horikoshii OT3 with AMP===
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ADP-dependent 6-phosphofructokinase from Pyrococcus horikoshii OT3: structure determination and biochemical characterization of PH1645.,Currie MA, Merino F, Skarina T, Wong AH, Singer A, Brown G, Savchenko A, Caniuguir A, Guixe V, Yakunin AF, Jia Z J Biol Chem. 2009 Aug 21;284(34):22664-71. Epub 2009 Jun 24. PMID:19553681<ref>PMID:19553681</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3drw" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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3DRW is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DRW OCA].
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*[[Phosphofructokinase 3D structures|Phosphofructokinase 3D structures]]
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[[Category: ADP-specific phosphofructokinase]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Pyrococcus horikoshii]]
[[Category: Pyrococcus horikoshii]]
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[[Category: Brown, G.]]
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[[Category: Brown G]]
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[[Category: Cuff, M E.]]
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[[Category: Cuff ME]]
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[[Category: Edwards, A M.]]
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[[Category: Edwards AM]]
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[[Category: Jia, Z.]]
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[[Category: Jia Z]]
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[[Category: Joachimiak, A.]]
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[[Category: Joachimiak A]]
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[[Category: Kochinyan, S.]]
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[[Category: Kochinyan S]]
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[[Category: MCSG, Midwest Center for Structural Genomics.]]
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[[Category: Savchenko A]]
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[[Category: Savchenko, A.]]
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[[Category: Singer AU]]
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[[Category: Singer, A U.]]
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[[Category: Skarina T]]
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[[Category: Skarina, T.]]
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[[Category: Yakunin AF]]
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[[Category: Yakunin, A F.]]
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[[Category: 6-phosphofructokinase]]
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[[Category: Adp]]
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[[Category: Amp]]
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[[Category: Cytoplasm]]
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[[Category: Glycolysis]]
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[[Category: Kinase]]
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[[Category: Magnesium]]
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[[Category: Mcsg]]
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[[Category: Metal-binding]]
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[[Category: Midwest center for structural genomic]]
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[[Category: Protein structure initiative]]
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[[Category: Psi-2]]
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[[Category: Pyrococcus horikoshii]]
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[[Category: Structural genomic]]
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[[Category: Transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 24 11:30:20 2008''
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Current revision

Crystal Structure of a Phosphofructokinase from Pyrococcus horikoshii OT3 with AMP

PDB ID 3drw

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