3fig

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{{Seed}}
 
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[[Image:3fig.jpg|left|200px]]
 
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==Crystal Structure of Leucine-bound LeuA from Mycobacterium tuberculosis==
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The line below this paragraph, containing "STRUCTURE_3fig", creates the "Structure Box" on the page.
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<StructureSection load='3fig' size='340' side='right'caption='[[3fig]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3fig]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FIG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FIG FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=LEU:LEUCINE'>LEU</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_3fig| PDB=3fig | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fig FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fig OCA], [https://pdbe.org/3fig PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fig RCSB], [https://www.ebi.ac.uk/pdbsum/3fig PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fig ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/LEU1_MYCTU LEU1_MYCTU] Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fi/3fig_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3fig ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The leucine biosynthetic pathway is essential for the growth of Mycobacterium tuberculosis and is a potential target for the design of new anti-tuberculosis drugs. The crystal structure of alpha-isopropylmalate synthase, which catalyzes the first committed step in this pathway, has been determined by multiwavelength anomalous dispersion methods and refined at 2.0-A resolution in complex with its substrate alpha-ketoisovalerate. The structure reveals a tightly associated, domain-swapped dimer in which each monomer comprises an (alpha/beta)(8) TIM barrel catalytic domain, a helical linker domain, and a regulatory domain of novel fold. Mutational and crystallographic data indicate the latter as the site for leucine feedback inhibition of activity. Domain swapping enables the linker domain of one monomer to sit over the catalytic domain of the other, inserting residues into the active site that may be important in catalysis. The alpha-ketoisovalerate substrate binds to an active site zinc ion, adjacent to a cavity that can accommodate acetyl-CoA. Sequence and structural similarities point to a catalytic mechanism similar to that of malate synthase and an evolutionary relationship with an aldolase that catalyzes the reverse reaction on a similar substrate.
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===Crystal Structure of Leucine-bound LeuA from Mycobacterium tuberculosis===
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Crystal structure of LeuA from Mycobacterium tuberculosis, a key enzyme in leucine biosynthesis.,Koon N, Squire CJ, Baker EN Proc Natl Acad Sci U S A. 2004 Jun 1;101(22):8295-300. Epub 2004 May 24. PMID:15159544<ref>PMID:15159544</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3fig" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_15159544}}, adds the Publication Abstract to the page
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*[[2-isopropylmalate synthase|2-isopropylmalate synthase]]
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(as it appears on PubMed at http://www.pubmed.gov), where 15159544 is the PubMed ID number.
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*[[2-isopropylmalate synthase 3D structures|2-isopropylmalate synthase 3D structures]]
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== References ==
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{{ABSTRACT_PUBMED_15159544}}
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<references/>
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__TOC__
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==About this Structure==
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</StructureSection>
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3FIG is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FIG OCA].
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[[Category: Large Structures]]
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==Reference==
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Crystal structure of LeuA from Mycobacterium tuberculosis, a key enzyme in leucine biosynthesis., Koon N, Squire CJ, Baker EN, Proc Natl Acad Sci U S A. 2004 Jun 1;101(22):8295-300. Epub 2004 May 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15159544 15159544]
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[[Category: 2-isopropylmalate synthase]]
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[[Category: Mycobacterium tuberculosis]]
[[Category: Mycobacterium tuberculosis]]
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[[Category: Baker, E N.]]
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[[Category: Baker EN]]
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[[Category: Koon, N.]]
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[[Category: Koon N]]
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[[Category: Squire, C J.]]
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[[Category: Squire CJ]]
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[[Category: Amino-acid biosynthesis]]
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[[Category: Branched-chain amino acid biosynthesis]]
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[[Category: Leucine biosynthesis]]
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[[Category: Tim barrel]]
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[[Category: Transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 24 11:39:02 2008''
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Current revision

Crystal Structure of Leucine-bound LeuA from Mycobacterium tuberculosis

PDB ID 3fig

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