1vpw

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(New page: 200px<br /><applet load="1vpw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1vpw, resolution 2.700&Aring;" /> '''STRUCTURE OF THE PU...)
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[[Image:1vpw.gif|left|200px]]<br /><applet load="1vpw" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1vpw, resolution 2.700&Aring;" />
 
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'''STRUCTURE OF THE PURR MUTANT, L54M, BOUND TO HYPOXANTHINE AND PURF OPERATOR DNA'''<br />
 
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==Overview==
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==STRUCTURE OF THE PURR MUTANT, L54M, BOUND TO HYPOXANTHINE AND PURF OPERATOR DNA==
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The crystal structure of the purine repressor mutant L54M bound to, hypoxanthine and to the purF operator provides a stereochemical, understanding of the high DNA affinity of this hinge helix mutant., Comparison of the PurR L54M-DNA complex to that of the wild type PurR-DNA, complex reveals that these purine repressors bind and kink DNA similarly, despite significant differences in their minor groove contacts and routes, to interdigitation of the central C.G:G.C base pair step. Modeling, studies, supported by genetic and biochemical data, show that the, stereochemistry of the backbone atoms of the abutting hinge helices, combined with the rigidity of the kinked base pair step constrain the, interdigitating residue to leucine or methionine for the LacI/GalR family, of transcription regulators.
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<StructureSection load='1vpw' size='340' side='right'caption='[[1vpw]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1vpw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VPW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1VPW FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HPA:HYPOXANTHINE'>HPA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1vpw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vpw OCA], [https://pdbe.org/1vpw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1vpw RCSB], [https://www.ebi.ac.uk/pdbsum/1vpw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1vpw ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PURR_ECOLI PURR_ECOLI] Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. In addition, it participates in the regulation or coregulation of genes involved in de novo pyrimidine nucleotide biosynthesis, salvage and uptake (pyrC, pyrD, carAB and codBA), and of several genes encoding enzymes necessary for nucleotide and polyamine biosynthesis (prsA, glyA, gcvTHP, speA, glnB). Binds to a 16-bp palindromic sequence located within the promoter region of pur regulon genes. The consensus binding sequence is 5'-ACGCAAACGTTTTCNT-3'. PurR is allosterically activated to bind its cognate DNA by binding the purine corepressors, hypoxanthine or guanine, thereby effecting transcription repression.<ref>PMID:2404765</ref> <ref>PMID:2211500</ref> <ref>PMID:1400170</ref> <ref>PMID:14741201</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vp/1vpw_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1vpw ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1VPW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with HPA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1VPW OCA].
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*[[Purine repressor|Purine repressor]]
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== References ==
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==Reference==
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<references/>
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The structure of PurR mutant L54M shows an alternative route to DNA kinking., Arvidson DN, Lu F, Faber C, Zalkin H, Brennan RG, Nat Struct Biol. 1998 Jun;5(6):436-41. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9628480 9628480]
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Arvidson, R.G.Brennan D.N.]]
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[[Category: Synthetic construct]]
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[[Category: Faber, C.]]
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[[Category: Arvidson DN]]
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[[Category: Lu, F.]]
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[[Category: Brennan RG]]
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[[Category: Zalkin, H.]]
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[[Category: Faber C]]
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[[Category: HPA]]
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[[Category: Lu F]]
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[[Category: complex (dna-binding protein/dna)]]
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[[Category: Zalkin H]]
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[[Category: dna-binding regulatory protein]]
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[[Category: minor groove bending]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 05:00:00 2007''
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Current revision

STRUCTURE OF THE PURR MUTANT, L54M, BOUND TO HYPOXANTHINE AND PURF OPERATOR DNA

PDB ID 1vpw

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