2ju4

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{{Seed}}
 
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[[Image:2ju4.png|left|200px]]
 
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==NMR structure of the gamma subunit of cGMP phosphodiesterase==
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The line below this paragraph, containing "STRUCTURE_2ju4", creates the "Structure Box" on the page.
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<StructureSection load='2ju4' size='340' side='right'caption='[[2ju4]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2ju4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JU4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JU4 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 100 models</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=RCY:(3R)-1-OXYL-2,2,5,5-TETRAMETHYL-1,3-BIPYRROLIDINE-2,5-DIONE'>RCY</scene></td></tr>
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{{STRUCTURE_2ju4| PDB=2ju4 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ju4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ju4 OCA], [https://pdbe.org/2ju4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ju4 RCSB], [https://www.ebi.ac.uk/pdbsum/2ju4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ju4 ProSAT], [https://www.topsan.org/Proteins/CESG/2ju4 TOPSAN]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CNRG_BOVIN CNRG_BOVIN] Participates in processes of transmission and amplification of the visual signal. cGMP-PDEs are the effector molecules in G-protein-mediated phototransduction in vertebrate rods and cones.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ju/2ju4_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ju4 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The retinal phosphodiesterase (PDE6) inhibitory gamma-subunit (PDEgamma) plays a central role in vertebrate phototransduction through alternate interactions with the catalytic alphabeta-subunits of PDE6 and the alpha-subunit of transducin (alpha(t)). Detailed structural analysis of PDEgamma has been hampered by its intrinsic disorder. We present here the NMR solution structure of PDEgamma, which reveals a loose fold with transient structural features resembling those seen previously in the x-ray structure of PDEgamma(46-87) when bound to alpha(t) in the transition-state complex. NMR mapping of the interaction between PDEgamma(46-87) and the chimeric PDE5/6 catalytic domain confirmed that C-terminal residues 74-87 of PDEgamma are involved in the association and demonstrated that its W70 indole group, which is critical for subsequent binding to alpha(t), is left free at this stage. These results indicate that the interaction between PDEgamma and alpha(t) during the phototransduction cascade involves the selection of preconfigured transient conformations.
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===NMR structure of the gamma subunit of cGMP phosphodiesterase===
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Intrinsically disordered gamma-subunit of cGMP phosphodiesterase encodes functionally relevant transient secondary and tertiary structure.,Song J, Guo LW, Muradov H, Artemyev NO, Ruoho AE, Markley JL Proc Natl Acad Sci U S A. 2008 Feb 5;105(5):1505-10. Epub 2008 Jan 29. PMID:18230733<ref>PMID:18230733</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2ju4" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_18230733}}, adds the Publication Abstract to the page
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*[[Phosphodiesterase 3D structures|Phosphodiesterase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 18230733 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_18230733}}
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__TOC__
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</StructureSection>
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==About this Structure==
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2JU4 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JU4 OCA].
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==Reference==
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Intrinsically disordered gamma-subunit of cGMP phosphodiesterase encodes functionally relevant transient secondary and tertiary structure., Song J, Guo LW, Muradov H, Artemyev NO, Ruoho AE, Markley JL, Proc Natl Acad Sci U S A. 2008 Feb 5;105(5):1505-10. Epub 2008 Jan 29. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18230733 18230733]
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[[Category: 3',5'-cyclic-nucleotide phosphodiesterase]]
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[[Category: Bos taurus]]
[[Category: Bos taurus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Pdbx_ordinal=, <PDBx:audit_author.]]
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[[Category: Guo LW]]
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[[Category: Center for eukaryotic structural genomic]]
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[[Category: Markley JL]]
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[[Category: Cesg]]
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[[Category: Ruoho AE]]
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[[Category: Cgmp]]
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[[Category: Song J]]
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[[Category: Hydrolase]]
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[[Category: Intrinsic disordered protein]]
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[[Category: Paramagnetic relaxation enhancement]]
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[[Category: Polymorphism]]
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[[Category: Protein structure initiative]]
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[[Category: Psi-2]]
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[[Category: Sensory transduction]]
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[[Category: Structural genomic]]
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[[Category: Vision]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 31 20:17:22 2008''
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Current revision

NMR structure of the gamma subunit of cGMP phosphodiesterase

PDB ID 2ju4

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