3emd

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{{Seed}}
 
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[[Image:3emd.png|left|200px]]
 
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==Wesselsbron virus Methyltransferase in complex with Sinefungin and 7MeGpppA==
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The line below this paragraph, containing "STRUCTURE_3emd", creates the "Structure Box" on the page.
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<StructureSection load='3emd' size='340' side='right'caption='[[3emd]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3emd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Wesselsbron_virus Wesselsbron virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EMD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3EMD FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTA:P1-7-METHYLGUANOSINE-P3-ADENOSINE-5,5-TRIPHOSPHATE'>GTA</scene>, <scene name='pdbligand=SFG:SINEFUNGIN'>SFG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_3emd| PDB=3emd | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3emd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3emd OCA], [https://pdbe.org/3emd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3emd RCSB], [https://www.ebi.ac.uk/pdbsum/3emd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3emd ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/D0VX01_WSLV D0VX01_WSLV]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The mRNA-capping process starts with the conversion of a 5'-triphosphate end into a 5'-diphosphate by an RNA triphosphatase, followed by the addition of a guanosine monophosphate unit in a 5'-5' phosphodiester bond by a guanylyltransferase. Methyltransferases are involved in the third step of the process, transferring a methyl group from S-adenosyl-l-methionine to N7-guanine (cap 0) and to the ribose 2'OH group (cap 1) of the first RNA nucleotide; capping is essential for mRNA stability and proper replication. In the genus Flavivirus, N7-methyltransferase and 2'O-methyltransferase activities have been recently associated with the N-terminal domain of the viral NS5 protein. In order to further characterize the series of enzymatic reactions that support capping, we analyzed the crystal structures of Wesselsbron virus methyltransferase in complex with the S-adenosyl-l-methionine cofactor, S-adenosyl-l-homocysteine (the product of the methylation reaction), Sinefungin (a molecular analogue of the enzyme cofactor), and three different cap analogues (GpppG, (N7Me)GpppG, and (N7Me)GpppA). The structural results, together with those on other flaviviral methyltransferases, show that the capped RNA analogues all bind to an RNA high-affinity binding site. However, lack of specific interactions between the enzyme and the first nucleotide of the RNA chain suggests the requirement of a minimal number of nucleotides following the cap to strengthen protein/RNA interaction. Our data also show that, following incubation with guanosine triphosphate, Wesselsbron virus methyltransferase displays a guanosine monophosphate molecule covalently bound to residue Lys28, hinting at possible implications for the transfer of a guanine group to ppRNA. The structures of the Wesselsbron virus methyltransferase complexes obtained are discussed in the context of a model for N7-methyltransferase and 2'O-methyltransferase activities.
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===Wesselsbron virus Methyltransferase in complex with Sinefungin and 7MeGpppA===
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Recognition of RNA cap in the Wesselsbron virus NS5 methyltransferase domain: implications for RNA-capping mechanisms in Flavivirus.,Bollati M, Milani M, Mastrangelo E, Ricagno S, Tedeschi G, Nonnis S, Decroly E, Selisko B, de Lamballerie X, Coutard B, Canard B, Bolognesi M J Mol Biol. 2009 Jan 9;385(1):140-52. Epub 2008 Oct 19. PMID:18976670<ref>PMID:18976670</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_18976670}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3emd" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 18976670 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_18976670}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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3EMD is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Wesselsbron_virus Wesselsbron virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EMD OCA].
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==Reference==
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Recognition of RNA Cap in the Wesselsbron Virus NS5 Methyltransferase Domain: Implications for RNA-Capping Mechanisms in Flavivirus., Bollati M, Milani M, Mastrangelo E, Ricagno S, Tedeschi G, Nonnis S, Decroly E, Selisko B, de Lamballerie X, Coutard B, Canard B, Bolognesi M, J Mol Biol. 2008 Oct 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18976670 18976670]
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[[Category: Wesselsbron virus]]
[[Category: Wesselsbron virus]]
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[[Category: Bollati, M.]]
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[[Category: Bollati M]]
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[[Category: Bolognesi, M.]]
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[[Category: Bolognesi M]]
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[[Category: Mastrangelo, E.]]
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[[Category: Mastrangelo E]]
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[[Category: Milani, M.]]
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[[Category: Milani M]]
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[[Category: Ricagno, S.]]
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[[Category: Ricagno S]]
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[[Category: Flavivirus]]
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[[Category: Guanylyltransferase]]
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[[Category: Methyltransferase]]
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[[Category: Rna capping]]
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[[Category: Viral enzyme structure]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 7 08:46:21 2009''
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Current revision

Wesselsbron virus Methyltransferase in complex with Sinefungin and 7MeGpppA

PDB ID 3emd

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