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3fla

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{{Seed}}
 
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[[Image:3fla.jpg|left|200px]]
 
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==RifR - Type II thioesterase from Rifamycin NRPS/PKS biosynthetic pathway - Form 1==
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The line below this paragraph, containing "STRUCTURE_3fla", creates the "Structure Box" on the page.
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<StructureSection load='3fla' size='340' side='right'caption='[[3fla]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3fla]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Amycolatopsis_mediterranei Amycolatopsis mediterranei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FLA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FLA FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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{{STRUCTURE_3fla| PDB=3fla | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fla FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fla OCA], [https://pdbe.org/3fla PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fla RCSB], [https://www.ebi.ac.uk/pdbsum/3fla PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fla ProSAT]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fl/3fla_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3fla ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Two thioesterases are commonly found in natural product biosynthetic clusters, a type I thioesterase (TEI) which is responsible for removing the final product from the biosynthetic complex, and a type II thioesterase (TEII) which is believed to perform housekeeping functions such as removing aberrant units from carrier domains. We present the crystal structure and the kinetic analysis of RifR, a TEII from the hybrid NRPS/PKS rifamycin biosynthetic cluster of Amycolatopsis mediterranei. Steady-state kinetics show RifR has a preference for the hydrolysis of acyl units from the phosphopantetheinyl arm (Ppant) of the acyl carrier domain, over the hydrolysis of acyl units from the Ppant of acyl-CoAs as well as a modest preference for the decarboxylated substrate mimics acetyl-CoA and propionyl-CoA over malonyl-CoA and methylmalonyl-CoA. Multiple RifR conformations and structural similarities to other thioesterases suggest that movement of a helical lid controls access of substrates to the active site of RifR.
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===RifR - Type II thioesterase from Rifamycin NRPS/PKS biosynthetic pathway - Form 1===
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Structure and functional analysis of RifR, the type II thioesterase from the rifamycin biosynthetic pathway.,Claxton HB, Akey DL, Silver MK, Admiraal SJ, Smith JL J Biol Chem. 2008 Dec 22. PMID:19103602<ref>PMID:19103602</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_19103602}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3fla" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 19103602 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19103602}}
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__TOC__
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</StructureSection>
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==About this Structure==
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3FLA is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Amycolatopsis_mediterranei Amycolatopsis mediterranei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FLA OCA].
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==Reference==
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Structure and functional analysis of RifR, the type II thioesterase from the rifamycin biosynthetic pathway., Claxton HB, Akey DL, Silver MK, Admiraal SJ, Smith JL, J Biol Chem. 2008 Dec 22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/19103602 19103602]
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[[Category: Amycolatopsis mediterranei]]
[[Category: Amycolatopsis mediterranei]]
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[[Category: Akey, D L.]]
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[[Category: Large Structures]]
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[[Category: Smith, J L.]]
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[[Category: Akey DL]]
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[[Category: Alpha-beta hydrolase thioesterase]]
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[[Category: Smith JL]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 7 09:36:17 2009''
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Current revision

RifR - Type II thioesterase from Rifamycin NRPS/PKS biosynthetic pathway - Form 1

PDB ID 3fla

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