1wa8

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1wa8" size="450" color="white" frame="true" align="right" spinBox="true" caption="1wa8" /> '''SOLUTION STRUCTURE OF THE CFP-10.ESAT-6 COMP...)
Current revision (13:33, 9 May 2024) (edit) (undo)
 
(16 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1wa8.gif|left|200px]]<br /><applet load="1wa8" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1wa8" />
 
-
'''SOLUTION STRUCTURE OF THE CFP-10.ESAT-6 COMPLEX. MAJOR VIRULENCE DETERMINANTS OF PATHOGENIC MYCOBACTERIA'''<br />
 
-
==Overview==
+
==Solution Structure of the CFP-10.ESAT-6 Complex. Major Virulence Determinants of Pathogenic Mycobacteria==
-
The secreted Mycobacterium tuberculosis complex proteins CFP-10 and ESAT-6, have recently been shown to play an essential role in tuberculosis, pathogenesis. We have determined the solution structure of the tight, 1:1, complex formed by CFP-10 and ESAT-6, and employed fluorescence microscopy, to demonstrate specific binding of the complex to the surface of, macrophage and monocyte cells. A striking feature of the complex is the, long flexible arm formed by the C-terminus of CFP-10, which was found to, be essential for binding to the surface of cells. The surface features of, the CFP-10.ESAT-6 complex, together with observed binding to specific host, cells, strongly suggest a key signalling role for the complex, in which, binding to cell surface receptors leads to modulation of host cell, behaviour to the advantage of the pathogen.
+
<StructureSection load='1wa8' size='340' side='right'caption='[[1wa8]]' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1wa8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv] and [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_variant_bovis Mycobacterium tuberculosis variant bovis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WA8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WA8 FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wa8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wa8 OCA], [https://pdbe.org/1wa8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wa8 RCSB], [https://www.ebi.ac.uk/pdbsum/1wa8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wa8 ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/ESXB_MYCBO ESXB_MYCBO] A secreted protein. Acts as a strong host T-cell antigen. Involved in translocation of bacteria from the host (human) phagolysosome to the host cytoplasm. Might serve as a chaperone to prevent uncontrolled membrane lysis by its partner EsxA.[UniProtKB:P9WNK5]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wa/1wa8_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wa8 ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The secreted Mycobacterium tuberculosis complex proteins CFP-10 and ESAT-6 have recently been shown to play an essential role in tuberculosis pathogenesis. We have determined the solution structure of the tight, 1:1 complex formed by CFP-10 and ESAT-6, and employed fluorescence microscopy to demonstrate specific binding of the complex to the surface of macrophage and monocyte cells. A striking feature of the complex is the long flexible arm formed by the C-terminus of CFP-10, which was found to be essential for binding to the surface of cells. The surface features of the CFP-10.ESAT-6 complex, together with observed binding to specific host cells, strongly suggest a key signalling role for the complex, in which binding to cell surface receptors leads to modulation of host cell behaviour to the advantage of the pathogen.
-
==About this Structure==
+
Structure and function of the complex formed by the tuberculosis virulence factors CFP-10 and ESAT-6.,Renshaw PS, Lightbody KL, Veverka V, Muskett FW, Kelly G, Frenkiel TA, Gordon SV, Hewinson RG, Burke B, Norman J, Williamson RA, Carr MD EMBO J. 2005 Jul 20;24(14):2491-8. Epub 2005 Jun 23. PMID:15973432<ref>PMID:15973432</ref>
-
1WA8 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mycobacterium_bovis Mycobacterium bovis] and [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1WA8 OCA].
+
-
==Reference==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
Structure and function of the complex formed by the tuberculosis virulence factors CFP-10 and ESAT-6., Renshaw PS, Lightbody KL, Veverka V, Muskett FW, Kelly G, Frenkiel TA, Gordon SV, Hewinson RG, Burke B, Norman J, Williamson RA, Carr MD, EMBO J. 2005 Jul 20;24(14):2491-8. Epub 2005 Jun 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15973432 15973432]
+
</div>
-
[[Category: Mycobacterium bovis]]
+
<div class="pdbe-citations 1wa8" style="background-color:#fffaf0;"></div>
-
[[Category: Mycobacterium tuberculosis]]
+
== References ==
-
[[Category: Protein complex]]
+
<references/>
-
[[Category: Burke, B.]]
+
__TOC__
-
[[Category: Carr, M.D.]]
+
</StructureSection>
-
[[Category: Frenkiel, T.A.]]
+
[[Category: Large Structures]]
-
[[Category: Gordon, S.V.]]
+
[[Category: Mycobacterium tuberculosis H37Rv]]
-
[[Category: Hewinson, R.G.]]
+
[[Category: Mycobacterium tuberculosis variant bovis]]
-
[[Category: Kelly, G.]]
+
[[Category: Burke B]]
-
[[Category: Lightbody, K.L.]]
+
[[Category: Carr MD]]
-
[[Category: Muskett, F.W.]]
+
[[Category: Frenkiel TA]]
-
[[Category: Norman, J.]]
+
[[Category: Gordon SV]]
-
[[Category: Renshaw, P.S.]]
+
[[Category: Hewinson RG]]
-
[[Category: TBSGC, TB.Structural.Genomics.Consortium.]]
+
[[Category: Kelly G]]
-
[[Category: Veverka, V.]]
+
[[Category: Lightbody KL]]
-
[[Category: Williamson, R.A.]]
+
[[Category: Muskett FW]]
-
[[Category: cfp-10]]
+
[[Category: Norman J]]
-
[[Category: esat-6]]
+
[[Category: Renshaw PS]]
-
[[Category: four helix bundle]]
+
[[Category: Veverka V]]
-
[[Category: helix-turn-helix]]
+
[[Category: Williamson RA]]
-
[[Category: mycobacteria]]
+
-
[[Category: nmr]]
+
-
[[Category: pathogenesis]]
+
-
[[Category: protein structure initiative]]
+
-
[[Category: psi]]
+
-
[[Category: solution structure]]
+
-
[[Category: tb structural genomics consortium]]
+
-
[[Category: tbsgc]]
+
-
[[Category: tuberculosis]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 05:16:28 2007''
+

Current revision

Solution Structure of the CFP-10.ESAT-6 Complex. Major Virulence Determinants of Pathogenic Mycobacteria

PDB ID 1wa8

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1wa8"
Personal tools