1wco

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(New page: 200px<br /><applet load="1wco" size="450" color="white" frame="true" align="right" spinBox="true" caption="1wco" /> '''THE SOLUTION STRUCTURE OF THE NISIN-LIPID II...)
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[[Image:1wco.gif|left|200px]]<br /><applet load="1wco" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1wco" />
 
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'''THE SOLUTION STRUCTURE OF THE NISIN-LIPID II COMPLEX'''<br />
 
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==Overview==
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==The solution structure of the nisin-lipid II complex==
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The emerging antibiotics-resistance problem has underlined the urgent need, for novel antimicrobial agents. Lantibiotics (lanthionine-containing, antibiotics) are promising candidates to alleviate this problem. Nisin, a, member of this family, has a unique pore-forming activity against, bacteria. It binds to lipid II, the essential precursor of cell wall, synthesis. As a result, the membrane permeabilization activity of nisin is, increased by three orders of magnitude. Here we report the solution, structure of the complex of nisin and lipid II. The structure shows a, novel lipid II-binding motif in which the pyrophosphate moiety of lipid II, is primarily coordinated by the N-terminal backbone amides of nisin via, intermolecular hydrogen bonds. This cage structure provides a rationale, for the conservation of the lanthionine rings among several lipid, II-binding lantibiotics. The structure of the pyrophosphate cage offers a, template for structure-based design of novel antibiotics.
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<StructureSection load='1wco' size='340' side='right'caption='[[1wco]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1wco]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactococcus_lactis Lactococcus lactis] and [https://en.wikipedia.org/wiki/Monarthropalpus_flavus Monarthropalpus flavus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1uzt 1uzt]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WCO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WCO FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DAL:D-ALANINE'>DAL</scene>, <scene name='pdbligand=DBB:D-ALPHA-AMINOBUTYRIC+ACID'>DBB</scene>, <scene name='pdbligand=DBU:(2Z)-2-AMINOBUT-2-ENOIC+ACID'>DBU</scene>, <scene name='pdbligand=DHA:2-AMINO-ACRYLIC+ACID'>DHA</scene>, <scene name='pdbligand=FDF:(2E,6E)-12-FLUORO-11-(FLUOROMETHYL)-3,7-DIMETHYLDODECA-2,6,10-TRIEN-1-YL+TRIHYDROGEN+DIPHOSPHATE'>FDF</scene>, <scene name='pdbligand=FGA:GAMMA-D-GLUTAMIC+ACID'>FGA</scene>, <scene name='pdbligand=MUB:N-ACETYLMURAMIC+ACID'>MUB</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wco FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wco OCA], [https://pdbe.org/1wco PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wco RCSB], [https://www.ebi.ac.uk/pdbsum/1wco PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wco ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/LANZ_LACLL LANZ_LACLL] Lanthionine-containing peptide antibiotic (lantibiotic) active on Gram-positive bacteria. The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores.
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==About this Structure==
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==See Also==
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1WCO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Lactococcus_lactis Lactococcus lactis] and [http://en.wikipedia.org/wiki/Monarthropalpus_flavus Monarthropalpus flavus] with NAG, MUB and FPP as [http://en.wikipedia.org/wiki/ligands ligands]. This structure superseeds the now removed PDB entry 1UZT. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1WCO OCA].
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*[[Nisin|Nisin]]
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__TOC__
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==Reference==
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</StructureSection>
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The nisin-lipid II complex reveals a pyrophosphate cage that provides a blueprint for novel antibiotics., Hsu ST, Breukink E, Tischenko E, Lutters MA, de Kruijff B, Kaptein R, Bonvin AM, van Nuland NA, Nat Struct Mol Biol. 2004 Oct;11(10):963-7. Epub 2004 Sep 12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15361862 15361862]
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[[Category: Lactococcus lactis]]
[[Category: Lactococcus lactis]]
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[[Category: Large Structures]]
[[Category: Monarthropalpus flavus]]
[[Category: Monarthropalpus flavus]]
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[[Category: Single protein]]
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[[Category: Bonvin AMJJ]]
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[[Category: Bonvin, A.M.J.J.]]
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[[Category: Breukink E]]
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[[Category: Breukink, E.]]
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[[Category: Hsu S-TD]]
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[[Category: Hsu, S.T.D.]]
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[[Category: Kaptein R]]
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[[Category: Kaptein, R.]]
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[[Category: Lutters MAG]]
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[[Category: Kruijff, B.De.]]
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[[Category: Tischenko E]]
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[[Category: Lutters, M.A.G.]]
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[[Category: De Kruijff B]]
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[[Category: Nuland, N.A.J.Van.]]
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[[Category: Van Nuland NAJ]]
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[[Category: Tischenko, E.]]
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[[Category: FPP]]
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[[Category: MUB]]
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[[Category: NAG]]
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[[Category: antibiotics]]
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[[Category: peptidoglycan]]
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[[Category: pore formation]]
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[[Category: pyrophosphate cage]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 05:18:40 2007''
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Current revision

The solution structure of the nisin-lipid II complex

PDB ID 1wco

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