3dpm

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{{Seed}}
 
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[[Image:3dpm.jpg|left|200px]]
 
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==Structure of mature CPAF complexed with lactacystin==
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The line below this paragraph, containing "STRUCTURE_3dpm", creates the "Structure Box" on the page.
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<StructureSection load='3dpm' size='340' side='right'caption='[[3dpm]], [[Resolution|resolution]] 2.35&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3dpm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlamydia_trachomatis Chlamydia trachomatis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DPM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3DPM FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.35&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LAS:N-ACETYL-S-({(2R,3S,4R)-3-HYDROXY-2-[(1S)-1-HYDROXY-2-METHYLPROPYL]-4-METHYL-5-OXOPYRROLIDIN-2-YL}CARBONYL)CYSTEINE'>LAS</scene></td></tr>
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{{STRUCTURE_3dpm| PDB=3dpm | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3dpm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dpm OCA], [https://pdbe.org/3dpm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3dpm RCSB], [https://www.ebi.ac.uk/pdbsum/3dpm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3dpm ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Y858_CHLTR Y858_CHLTR]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dp/3dpm_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3dpm ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The obligate intracellular pathogen Chlamydia trachomatis is the most common cause of sexually transmitted bacterial disease. It secretes a protease known as chlamydial protease/proteasome-like activity factor (CPAF) that degrades many host molecules and plays a major role in Chlamydia pathogenesis. Here, we show that mature CPAF is a homodimer of the catalytic domains, each of which comprises two distinct subunits. Dormancy of the CPAF zymogen is maintained by an internal inhibitory segment that binds the CPAF active site and blocks its homodimerization. CPAF activation is initiated by trans-autocatalytic cleavage, which induces homodimerization and conformational changes that assemble the catalytic triad. This assembly leads to two autocatalytic cleavages and removal of the inhibitory segment, enabling full CPAF activity. CPAF is covalently bound and inhibited by the proteasome inhibitor lactacystin. These results reveal the activation mechanism of the CPAF serine protease and suggest new opportunities for anti-Chlamydia drug development.
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===Structure of mature CPAF complexed with lactacystin===
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Structural basis for activation and inhibition of the secreted chlamydia protease CPAF.,Huang Z, Feng Y, Chen D, Wu X, Huang S, Wang X, Xiao X, Li W, Huang N, Gu L, Zhong G, Chai J Cell Host Microbe. 2008 Dec 11;4(6):529-42. PMID:19064254<ref>PMID:19064254</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_19064254}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3dpm" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 19064254 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19064254}}
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__TOC__
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</StructureSection>
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==About this Structure==
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3DPM is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Chlamydia_trachomatis Chlamydia trachomatis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DPM OCA].
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==Reference==
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Structural basis for activation and inhibition of the secreted chlamydia protease CPAF., Huang Z, Feng Y, Chen D, Wu X, Huang S, Wang X, Xiao X, Li W, Huang N, Gu L, Zhong G, Chai J, Cell Host Microbe. 2008 Dec 11;4(6):529-42. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/19064254 19064254]
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[[Category: Chlamydia trachomatis]]
[[Category: Chlamydia trachomatis]]
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[[Category: Chai, J.]]
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[[Category: Large Structures]]
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[[Category: Huang, Z.]]
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[[Category: Chai J]]
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[[Category: Complex]]
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[[Category: Huang Z]]
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[[Category: Cpaf]]
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[[Category: Dimer]]
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[[Category: Lactacystin]]
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[[Category: Transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 14 13:17:29 2009''
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Current revision

Structure of mature CPAF complexed with lactacystin

PDB ID 3dpm

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OCA

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