3e9g

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{{Seed}}
 
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[[Image:3e9g.png|left|200px]]
 
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==Crystal structure long-form (residue1-124) of Eaf3 chromo domain==
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The line below this paragraph, containing "STRUCTURE_3e9g", creates the "Structure Box" on the page.
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<StructureSection load='3e9g' size='340' side='right'caption='[[3e9g]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3e9g]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E9G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3E9G FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3e9g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3e9g OCA], [https://pdbe.org/3e9g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3e9g RCSB], [https://www.ebi.ac.uk/pdbsum/3e9g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3e9g ProSAT]</span></td></tr>
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{{STRUCTURE_3e9g| PDB=3e9g | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/EAF3_YEAST EAF3_YEAST] Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of selected genes principally by acetylation of nucleosomal histone H4 and H2A. The NuA4 complex is also involved in DNA repair.<ref>PMID:11036083</ref> <ref>PMID:14701747</ref> <ref>PMID:15045029</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e9/3e9g_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3e9g ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Eaf3 is a component of both NuA4 histone acetyltransferase and Rpd3S histone deacetylase complexes in Saccharomyces cerevisiae. It is involved in the regulation of the global pattern of histone acetylation that distinguishes promoters from coding regions. Eaf3 contains a chromo domain at the N terminus that can bind to methylated Lys-36 of histone H3 (H3K36). We report here the crystal structures of the Eaf3 chromo domain in two truncation forms. Unlike the typical HP1 and Polycomb chromo domains, which contain a large groove to bind the modified histone tail, the Eaf3 chromo domain assumes an autoinhibited chromo barrel domain similar to the human MRG15 chromo domain. Compared with other chromo domains, the Eaf3 chromo domain contains a unique 38-residue insertion that folds into two short beta-strands and a long flexible loop to flank the beta-barrel core. Both isothermal titration calorimetry and surface plasmon resonance studies indicate that the interaction between the Eaf3 chromo domain and the trimethylated H3K36 peptide is relatively weak, with a K(D) of approximately 10(-4) m. NMR titration studies demonstrate that the methylated H3K36 peptide is bound to the cleft formed by the C-terminal alpha-helix and the beta-barrel core. Site-directed mutagenesis study and in vitro binding assay results show that the conserved aromatic residues Tyr-23, Tyr-81, Trp-84, and Trp-88, which form a hydrophobic pocket at one end of the beta-barrel, are essential for the binding of the methylated H3K36. These results reveal the molecular mechanism of the recognition and binding of the methylated H3K36 by Eaf3 and provide new insights into the functional roles of the Eaf3 chromo domain.
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===Crystal structure long-form (residue1-124) of Eaf3 chromo domain===
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Molecular basis of the interaction of Saccharomyces cerevisiae Eaf3 chromo domain with methylated H3K36.,Sun B, Hong J, Zhang P, Dong X, Shen X, Lin D, Ding J J Biol Chem. 2008 Dec 26;283(52):36504-12. Epub 2008 Nov 4. PMID:18984594<ref>PMID:18984594</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_18984594}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3e9g" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 18984594 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_18984594}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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3E9G is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E9G OCA].
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==Reference==
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Molecular Basis of the Interaction of Saccharomyces cerevisiae Eaf3 Chromo Domain with Methylated H3K36., Sun B, Hong J, Zhang P, Dong X, Shen X, Lin D, Ding J, J Biol Chem. 2008 Dec 26;283(52):36504-12. Epub 2008 Nov 4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18984594 18984594]
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[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
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[[Category: Ding J]]
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[[Category: Pdbx_ordinal=, <PDBx:audit_author.]]
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[[Category: Hong J]]
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[[Category: Chromatin regulator]]
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[[Category: Lin D]]
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[[Category: Chromatin remodeling]]
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[[Category: Sun B]]
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[[Category: Chromo domain]]
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[[Category: Zhang P]]
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[[Category: Dna damage]]
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[[Category: Dna repair]]
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[[Category: Eaf3]]
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[[Category: Nucleus]]
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[[Category: Transcription]]
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[[Category: Transcription factor]]
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[[Category: Transcription regulation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 14 20:33:11 2009''
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Current revision

Crystal structure long-form (residue1-124) of Eaf3 chromo domain

PDB ID 3e9g

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