3euh

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (00:24, 28 December 2023) (edit) (undo)
 
(9 intermediate revisions not shown.)
Line 1: Line 1:
-
{{Seed}}
 
-
[[Image:3euh.jpg|left|200px]]
 
-
<!--
+
==Crystal Structure of the MukE-MukF Complex==
-
The line below this paragraph, containing "STRUCTURE_3euh", creates the "Structure Box" on the page.
+
<StructureSection load='3euh' size='340' side='right'caption='[[3euh]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[3euh]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EUH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3EUH FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLY:GLYCINE'>GLY</scene></td></tr>
-
{{STRUCTURE_3euh| PDB=3euh | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3euh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3euh OCA], [https://pdbe.org/3euh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3euh RCSB], [https://www.ebi.ac.uk/pdbsum/3euh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3euh ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/MUKF_ECOLI MUKF_ECOLI] Involved in chromosome condensation, segregation and cell cycle progression. May participate in facilitating chromosome segregation by condensation DNA from both sides of a centrally located replisome during cell division. Not required for mini-F plasmid partitioning. Probably acts via its interaction with MukB and MukE. Overexpression results in anucleate cells. It has a calcium binding activity.<ref>PMID:8602138</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eu/3euh_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3euh ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Condensins are key mediators of chromosome condensation across organisms. Like other condensins, the bacterial MukBEF condensin complex consists of an SMC family protein dimer containing two ATPase head domains, MukB, and two interacting subunits, MukE and MukF. We report complete structural views of the intersubunit interactions of this condensin along with ensuing studies that reveal a role for the ATPase activity of MukB. MukE and MukF together form an elongated dimeric frame, and MukF's C-terminal winged-helix domains (C-WHDs) bind MukB heads to constitute closed ring-like structures. Surprisingly, one of the two bound C-WHDs is forced to detach upon ATP-mediated engagement of MukB heads. This detachment reaction depends on the linker segment preceding the C-WHD, and mutations on the linker restrict cell growth. Thus ATP-dependent transient disruption of the MukB-MukF interaction, which creates openings in condensin ring structures, is likely to be a critical feature of the functional mechanism of condensins.
-
===Crystal Structure of the MukE-MukF Complex===
+
Structural studies of a bacterial condensin complex reveal ATP-dependent disruption of intersubunit interactions.,Woo JS, Lim JH, Shin HC, Suh MK, Ku B, Lee KH, Joo K, Robinson H, Lee J, Park SY, Ha NC, Oh BH Cell. 2009 Jan 9;136(1):85-96. PMID:19135891<ref>PMID:19135891</ref>
-
 
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
==About this Structure==
+
</div>
-
3EUH is a 6 chains structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EUH OCA].
+
<div class="pdbe-citations 3euh" style="background-color:#fffaf0;"></div>
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
-
[[Category: Ha, N C.]]
+
[[Category: Escherichia coli K-12]]
-
[[Category: Ku, B.]]
+
[[Category: Large Structures]]
-
[[Category: Oh, B H.]]
+
[[Category: Ha NC]]
-
[[Category: Suh, M K.]]
+
[[Category: Ku B]]
-
[[Category: Woo, J S.]]
+
[[Category: Oh BH]]
-
[[Category: Calcium]]
+
[[Category: Suh MK]]
-
[[Category: Cell cycle]]
+
[[Category: Woo JS]]
-
[[Category: Cell division]]
+
-
[[Category: Chromosome condensation]]
+
-
[[Category: Chromosome partition]]
+
-
[[Category: Condensin]]
+
-
[[Category: Cytoplasm]]
+
-
[[Category: Dna condensation]]
+
-
[[Category: Kleisin]]
+
-
[[Category: Muke]]
+
-
[[Category: Mukf]]
+
-
[[Category: Non-smc subunit]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 21 10:47:23 2009''
+

Current revision

Crystal Structure of the MukE-MukF Complex

PDB ID 3euh

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3euh"
Personal tools