3f2c

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{{Seed}}
 
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[[Image:3f2c.jpg|left|200px]]
 
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==DNA Polymerase PolC from Geobacillus kaustophilus complex with DNA, dGTP and Mn==
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The line below this paragraph, containing "STRUCTURE_3f2c", creates the "Structure Box" on the page.
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<StructureSection load='3f2c' size='340' side='right'caption='[[3f2c]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3f2c]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_kaustophilus Geobacillus kaustophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3F2C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3F2C FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DGT:2-DEOXYGUANOSINE-5-TRIPHOSPHATE'>DGT</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_3f2c| PDB=3f2c | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3f2c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3f2c OCA], [https://pdbe.org/3f2c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3f2c RCSB], [https://www.ebi.ac.uk/pdbsum/3f2c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3f2c ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q5L0J3_GEOKA Q5L0J3_GEOKA] Required for replicative DNA synthesis. This DNA polymerase also exhibits 3' to 5' exonuclease activity (By similarity).[HAMAP-Rule:MF_00356][SAAS:SAAS029460_004_008054]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f2/3f2c_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3f2c ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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PolC is the polymerase responsible for genome duplication in many Gram-positive bacteria and represents an attractive target for antibacterial development. We have determined the 2.4-A resolution crystal structure of Geobacillus kaustophilus PolC in a ternary complex with DNA and dGTP. The structure reveals nascent base pair interactions that lead to highly accurate nucleotide incorporation. A unique beta-strand motif in the PolC thumb domain contacts the minor groove, allowing replication errors to be sensed up to 8 nt upstream of the active site. PolC exhibits the potential for large-scale conformational flexibility, which could encompass the catalytic residues. The structure suggests a mechanism by which the active site can communicate with the rest of the replisome to trigger proofreading after nucleotide misincorporation, leading to an integrated model for controlling the dynamic switch between replicative and repair polymerases. This ternary complex of a cellular replicative polymerase affords insights into polymerase fidelity, evolution, and structural diversity.
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===DNA Polymerase PolC from Geobacillus kaustophilus complex with DNA, dGTP and Mn===
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Structure of PolC reveals unique DNA binding and fidelity determinants.,Evans RJ, Davies DR, Bullard JM, Christensen J, Green LS, Guiles JW, Pata JD, Ribble WK, Janjic N, Jarvis TC Proc Natl Acad Sci U S A. 2008 Dec 30;105(52):20695-700. Epub 2008 Dec 23. PMID:19106298<ref>PMID:19106298</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3f2c" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_19106298}}, adds the Publication Abstract to the page
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*[[DNA polymerase 3D structures|DNA polymerase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 19106298 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19106298}}
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__TOC__
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</StructureSection>
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==About this Structure==
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3F2C is a 3 chains structure of sequences from [http://en.wikipedia.org/wiki/Geobacillus_kaustophilus Geobacillus kaustophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3F2C OCA].
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==Reference==
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<ref group="xtra">PMID:19106298</ref><references group="xtra"/>
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[[Category: DNA-directed DNA polymerase]]
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[[Category: Geobacillus kaustophilus]]
[[Category: Geobacillus kaustophilus]]
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[[Category: Bullard, J M.]]
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[[Category: Large Structures]]
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[[Category: Christensen, J.]]
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[[Category: Bullard JM]]
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[[Category: Davies, D R.]]
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[[Category: Christensen J]]
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[[Category: Evans, R J.]]
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[[Category: Davies DR]]
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[[Category: Green, L S.]]
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[[Category: Evans RJ]]
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[[Category: Guiles, J W.]]
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[[Category: Green LS]]
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[[Category: Janjic, N.]]
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[[Category: Guiles JW]]
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[[Category: Jarvis, T C.]]
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[[Category: Janjic N]]
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[[Category: Ribble, W K.]]
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[[Category: Jarvis TC]]
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[[Category: Dna polymerase c]]
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[[Category: Ribble WK]]
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[[Category: Dna polymerase iii]]
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[[Category: Gram-positive]]
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[[Category: Protein-dna complex]]
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[[Category: Replicative polymerase]]
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[[Category: Ternary complex]]
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[[Category: Transferase/dna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 21 10:48:17 2009''
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Current revision

DNA Polymerase PolC from Geobacillus kaustophilus complex with DNA, dGTP and Mn

PDB ID 3f2c

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