1wkb

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(New page: 200px<br /><applet load="1wkb" size="450" color="white" frame="true" align="right" spinBox="true" caption="1wkb, resolution 2.05&Aring;" /> '''Crystal Structure of...)
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[[Image:1wkb.gif|left|200px]]<br /><applet load="1wkb" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1wkb, resolution 2.05&Aring;" />
 
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'''Crystal Structure of Leucyl-tRNA Synthetase from the Archaeon Pyrococcus horikoshii Reveals a Novel Editing Domain Orientation'''<br />
 
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==Overview==
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==Crystal Structure of Leucyl-tRNA Synthetase from the Archaeon Pyrococcus horikoshii Reveals a Novel Editing Domain Orientation==
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The editing domains of the closely homologous leucyl, isoleucyl, and, valyl-tRNA synthetases (LeuRS, IleRS, and ValRS, respectively) contribute, to accurate aminoacylation, by hydrolyzing misformed non-cognate, aminoacyl-tRNAs. The editing domain is inserted at the same point of the, sequence in IleRS, ValRS, and the archaeal/eukaryal LeuRS, but at a, distinct point in the bacterial LeuRS. Here, we showed that LeuRS from the, archaeon Pyrococcus horikoshii has editing activity against the nearly, cognate isoleucine. The conserved Asp332 in the editing domain is crucial, for this activity. A deletion mutant lacking the C-terminal region has, only negligible aminoacylation activity, but retains the full activity of, adenylate synthesis and editing. We determined the crystal structure of, this editing-active, truncated form of P.horikoshii LeuRS at 2.1 A, resolution. The structure revealed that it has a novel editing domain, orientation. The editing domain of P.horikoshii LeuRS is rotated by, approximately 180 degrees (rotational state II), with the, two-beta-stranded linker untwisted by a half-turn, as compared to those in, IleRS and ValRS (rotational state I). This editing domain rotational state, in the archaeal LeuRS is similar to that in the bacterial LeuRS. However, because of the insertion point difference, the orientation of the editing, domain relative to the enzyme core in the archaeal LeuRS differs, completely from that in the bacterial LeuRS. An insertion region specific, to the archaeal/eukaryal LeuRS editing domains interacts with the enzyme, core and stabilizes the unique orientation. Thus, we established that, there are three types of editing domain orientations relative to the, enzyme core, depending on the combination of the editing domain insertion, point (i or ii) and the rotational state (I or II): [i, I] for IleRS and, ValRS, [ii, II] for the bacterial LeuRS, and now [i, II] for the, archaeal/eukaryal LeuRS.
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<StructureSection load='1wkb' size='340' side='right'caption='[[1wkb]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1wkb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WKB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WKB FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wkb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wkb OCA], [https://pdbe.org/1wkb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wkb RCSB], [https://www.ebi.ac.uk/pdbsum/1wkb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wkb ProSAT], [https://www.topsan.org/Proteins/RSGI/1wkb TOPSAN]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SYL_PYRHO SYL_PYRHO]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wk/1wkb_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wkb ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1WKB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Leucine--tRNA_ligase Leucine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.4 6.1.1.4] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1WKB OCA].
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*[[Aminoacyl tRNA synthetase 3D structures|Aminoacyl tRNA synthetase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of leucyl-tRNA synthetase from the archaeon Pyrococcus horikoshii reveals a novel editing domain orientation., Fukunaga R, Yokoyama S, J Mol Biol. 2005 Feb 11;346(1):57-71. Epub 2004 Dec 19. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15663927 15663927]
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[[Category: Large Structures]]
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[[Category: Leucine--tRNA ligase]]
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[[Category: Pyrococcus horikoshii]]
[[Category: Pyrococcus horikoshii]]
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[[Category: Single protein]]
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[[Category: Fukunaga R]]
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[[Category: Fukunaga, R.]]
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[[Category: Yokoyama S]]
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[[Category: RSGI, RIKEN.Structural.Genomics/Proteomics.Initiative.]]
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[[Category: Yokoyama, S.]]
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[[Category: SO4]]
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[[Category: amino acid]]
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[[Category: aminoacyl-trna]]
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[[Category: aminoacylation]]
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[[Category: editing]]
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[[Category: leucine]]
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[[Category: leucyl-trna synthetase]]
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[[Category: pyrococcus horikoshii]]
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[[Category: riken structural genomics/proteomics initiative]]
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[[Category: rsgi]]
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[[Category: structural genomics]]
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[[Category: trnaleu]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 05:29:01 2007''
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Current revision

Crystal Structure of Leucyl-tRNA Synthetase from the Archaeon Pyrococcus horikoshii Reveals a Novel Editing Domain Orientation

PDB ID 1wkb

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