2vwp

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{{Seed}}
 
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[[Image:2vwp.png|left|200px]]
 
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==Haloferax mediterranei glucose dehydrogenase in complex with NADPH and Zn.==
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The line below this paragraph, containing "STRUCTURE_2vwp", creates the "Structure Box" on the page.
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<StructureSection load='2vwp' size='340' side='right'caption='[[2vwp]], [[Resolution|resolution]] 2.01&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2vwp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Haloferax_mediterranei Haloferax mediterranei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VWP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VWP FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.01&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_2vwp| PDB=2vwp | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vwp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vwp OCA], [https://pdbe.org/2vwp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vwp RCSB], [https://www.ebi.ac.uk/pdbsum/2vwp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vwp ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GLCDH_HALMT GLCDH_HALMT] Catalyzes the NAD(P)(+)-dependent oxidation of D-glucose to D-gluconate. Displays broad substrate specificity since it is able to catalyze the oxidation of a number of alternative aldose sugars, such as D-xylose, D-galactose, and D-fucose, to the corresponding glyconate. Can utilize both NAD(+) and NADP(+) as electron acceptor, with a preference for NADP(+). Physiologically, seems to be involved in the degradation of glucose through a modified Entner-Doudoroff pathway.<ref>PMID:11425479</ref> <ref>PMID:8925901</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vw/2vwp_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2vwp ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Despite being the subject of intensive investigations, many aspects of the mechanism of the zinc-dependent medium chain alcohol dehydrogenase (MDR) superfamily remain contentious. We have determined the high-resolution structures of a series of binary and ternary complexes of glucose dehydrogenase, an MDR enzyme from Haloferax mediterranei. In stark contrast to the textbook MDR mechanism in which the zinc ion is proposed to remain stationary and attached to a common set of protein ligands, analysis of these structures reveals that in each complex, there are dramatic differences in the nature of the zinc ligation. These changes arise as a direct consequence of linked movements of the zinc ion, a zinc-bound bound water molecule, and the substrate during progression through the reaction. These results provide evidence for the molecular basis of proton traffic during catalysis, a structural explanation for pentacoordinate zinc ion intermediates, a unifying view for the observed patterns of metal ligation in the MDR family, and highlight the importance of dynamic fluctuations at the metal center in changing the electrostatic potential in the active site, thereby influencing the proton traffic and hydride transfer events.
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===HALOFERAX MEDITERRANEI GLUCOSE DEHYDROGENASE IN COMPLEX WITH NADPH AND ZN.===
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Active site dynamics in the zinc-dependent medium chain alcohol dehydrogenase superfamily.,Baker PJ, Britton KL, Fisher M, Esclapez J, Pire C, Bonete MJ, Ferrer J, Rice DW Proc Natl Acad Sci U S A. 2009 Jan 8. PMID:19131516<ref>PMID:19131516</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_19131516}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2vwp" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 19131516 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19131516}}
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__TOC__
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</StructureSection>
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==About this Structure==
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2VWP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Haloferax_mediterranei Haloferax mediterranei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VWP OCA].
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==Reference==
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Active site dynamics in the zinc-dependent medium chain alcohol dehydrogenase superfamily., Baker PJ, Britton KL, Fisher M, Esclapez J, Pire C, Bonete MJ, Ferrer J, Rice DW, Proc Natl Acad Sci U S A. 2009 Jan 8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/19131516 19131516]
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[[Category: Glucose 1-dehydrogenase]]
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[[Category: Haloferax mediterranei]]
[[Category: Haloferax mediterranei]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Pdbx_ordinal=, <PDBx:audit_author.]]
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[[Category: Baker PJ]]
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[[Category: Alcohol dehydrogenase]]
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[[Category: Bonete MJ]]
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[[Category: Glucose dehydrogenase]]
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[[Category: Britton KL]]
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[[Category: Oxidoreductase]]
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[[Category: Esclapez J]]
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[[Category: Zinc dependent medium chain alcohol dehydrogenase family]]
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[[Category: Ferrer J]]
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[[Category: Fisher M]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 21 20:35:07 2009''
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[[Category: Pire C]]
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[[Category: Rice DW]]

Current revision

Haloferax mediterranei glucose dehydrogenase in complex with NADPH and Zn.

PDB ID 2vwp

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