2zy3

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[[Image:2zy3.jpg|left|200px]]
 
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==dodecameric L-aspartate beta-decarboxylase==
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The line below this paragraph, containing "STRUCTURE_2zy3", creates the "Structure Box" on the page.
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<StructureSection load='2zy3' size='340' side='right'caption='[[2zy3]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2zy3]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Alcaligenes_faecalis_subsp._faecalis Alcaligenes faecalis subsp. faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZY3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZY3 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>
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{{STRUCTURE_2zy3| PDB=2zy3 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zy3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zy3 OCA], [https://pdbe.org/2zy3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zy3 RCSB], [https://www.ebi.ac.uk/pdbsum/2zy3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zy3 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ASDA_COMTE ASDA_COMTE] Bifunctional enzyme that has both L-aspartate decarboxylase and transaminase activity.<ref>PMID:19368885</ref> [PDB:2ZY3]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zy/2zy3_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2zy3 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The type-I PLP enzyme l-aspartate beta-decarboxylase converts aspartate to alanine and CO(2). Similar to the homodimeric aminotransferases, its protein subunit comprises a large and a small domain, of 410 and 120 residues, respectively. The crystal structure reveals a dodecamer made of six identical dimers arranged in a truncated tetrahedron whose assembly involves tetramer and hexamer as intermediates. The additional helical motifs I and II participate in the oligomer formation. Triple mutations of S67R/Y68R/M69R or S67E/Y68E/M69E in motif I produced an inactive dimer. The PLP is bound covalently to Lys315 in the active site, while its phosphate group interacts with a neighboring Tyr134. Removal of the bulky side chain of Arg37, which overhangs the PLP group, improved the substrate affinity. Mutations in flexible regions produced the more active K17A and the completely inactive R487A. The structure also suggests that substrate binding triggers conformational changes essential for catalyzing the reaction.
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===dodecameric L-aspartate beta-decarboxylase===
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Structure, assembly, and mechanism of a PLP-dependent dodecameric L-aspartate beta-decarboxylase.,Chen HJ, Ko TP, Lee CY, Wang NC, Wang AH Structure. 2009 Apr 15;17(4):517-29. PMID:19368885<ref>PMID:19368885</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2zy3" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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2ZY3 is a 6 chains structure of sequences from [http://en.wikipedia.org/wiki/Alcaligenes_faecalis_subsp._faecalis Alcaligenes faecalis subsp. faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZY3 OCA].
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*[[Aspartate decarboxylase 3D structures|Aspartate decarboxylase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Alcaligenes faecalis subsp. faecalis]]
[[Category: Alcaligenes faecalis subsp. faecalis]]
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[[Category: Aspartate 4-decarboxylase]]
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[[Category: Large Structures]]
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[[Category: Chen, H J.]]
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[[Category: Chen H-J]]
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[[Category: Ko, T P.]]
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[[Category: Ko T-P]]
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[[Category: Lee, C Y.]]
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[[Category: Lee C-Y]]
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[[Category: Wang, A H.J.]]
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[[Category: Wang AH-J]]
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[[Category: Wang, N C.]]
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[[Category: Wang N-C]]
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[[Category: Aminotransferase]]
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[[Category: Lyase]]
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[[Category: Pyridoxal 5'-phosphate]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 28 09:45:36 2009''
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Current revision

dodecameric L-aspartate beta-decarboxylase

PDB ID 2zy3

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