1woc

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(New page: 200px<br /><applet load="1woc" size="450" color="white" frame="true" align="right" spinBox="true" caption="1woc, resolution 2.&Aring;" /> '''Crystal structure of P...)
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[[Image:1woc.gif|left|200px]]<br /><applet load="1woc" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1woc, resolution 2.&Aring;" />
 
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'''Crystal structure of PriB'''<br />
 
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==Overview==
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==Crystal structure of PriB==
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PriB is not only an essential protein necessary for the replication, restart on the collapsed and disintegrated replication fork, but also an, important protein for assembling of primosome onto PhiX174 genomic DNA, during replication initiation. Here we report a 2.0-A-resolution X-ray, structure of a biologically functional form of PriB from Escherichia coli., The crystal structure revealed that despite a low level of primary, sequence identity, the PriB monomer, as well as the dimeric form, are, structurally identical to the N-terminal DNA-binding domain of the, single-stranded DNA-binding protein (SSB) from Escherichia coli, which, possesses an oligonucleotides-binding-fold. The oligonucleotide-PriB, complex model based on the oligonucleotides-SSB complex structure, suggested that PriB had a DNA-binding pocket conserved in SSB from, Escherichia coli and might bind to single-stranded DNA in the manner of, SSB. Furthermore, surface plasmon resonance analysis and fluorescence, measurements demonstrated that PriB binds single-stranded DNA with high, affinity, by involving tryptophan residue. The significance of these, results with respect to the functional role of PriB in the assembly of, primosome is discussed.
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<StructureSection load='1woc' size='340' side='right'caption='[[1woc]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1woc]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WOC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WOC FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1woc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1woc OCA], [https://pdbe.org/1woc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1woc RCSB], [https://www.ebi.ac.uk/pdbsum/1woc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1woc ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PRIB_ECOLI PRIB_ECOLI] Binds single-stranded DNA at the primosome assembly site (PAS). During primosome assembly it facilitates the complex formation between PriA and DnaT.<ref>PMID:1856227</ref> <ref>PMID:1646811</ref> <ref>PMID:8366072</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wo/1woc_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1woc ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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PriB is not only an essential protein necessary for the replication restart on the collapsed and disintegrated replication fork, but also an important protein for assembling of primosome onto PhiX174 genomic DNA during replication initiation. Here we report a 2.0-A-resolution X-ray structure of a biologically functional form of PriB from Escherichia coli. The crystal structure revealed that despite a low level of primary sequence identity, the PriB monomer, as well as the dimeric form, are structurally identical to the N-terminal DNA-binding domain of the single-stranded DNA-binding protein (SSB) from Escherichia coli, which possesses an oligonucleotides-binding-fold. The oligonucleotide-PriB complex model based on the oligonucleotides-SSB complex structure suggested that PriB had a DNA-binding pocket conserved in SSB from Escherichia coli and might bind to single-stranded DNA in the manner of SSB. Furthermore, surface plasmon resonance analysis and fluorescence measurements demonstrated that PriB binds single-stranded DNA with high affinity, by involving tryptophan residue. The significance of these results with respect to the functional role of PriB in the assembly of primosome is discussed.
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==About this Structure==
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Crystal structure of a biologically functional form of PriB from Escherichia coli reveals a potential single-stranded DNA-binding site.,Shioi S, Ose T, Maenaka K, Shiroishi M, Abe Y, Kohda D, Katayama T, Ueda T Biochem Biophys Res Commun. 2005 Jan 28;326(4):766-76. PMID:15607735<ref>PMID:15607735</ref>
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1WOC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1WOC OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Crystal structure of a biologically functional form of PriB from Escherichia coli reveals a potential single-stranded DNA-binding site., Shioi S, Ose T, Maenaka K, Shiroishi M, Abe Y, Kohda D, Katayama T, Ueda T, Biochem Biophys Res Commun. 2005 Jan 28;326(4):766-76. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15607735 15607735]
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</div>
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[[Category: Escherichia coli]]
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<div class="pdbe-citations 1woc" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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== References ==
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[[Category: Abe, Y.]]
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<references/>
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[[Category: Katayama, T.]]
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__TOC__
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[[Category: Kohda, D.]]
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</StructureSection>
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[[Category: Maenaka, K.]]
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[[Category: Escherichia coli K-12]]
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[[Category: Ose, T.]]
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[[Category: Large Structures]]
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[[Category: Shioi, S.]]
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[[Category: Abe Y]]
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[[Category: Ueda, T.]]
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[[Category: Katayama T]]
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[[Category: oligonucleotide binding fold]]
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[[Category: Kohda D]]
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[[Category: Maenaka K]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 05:33:46 2007''
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[[Category: Ose T]]
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[[Category: Shioi S]]
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[[Category: Ueda T]]

Current revision

Crystal structure of PriB

PDB ID 1woc

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