1wor

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(New page: 200px<br /><applet load="1wor" size="450" color="white" frame="true" align="right" spinBox="true" caption="1wor, resolution 1.95&Aring;" /> '''Crystal Structure of...)
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[[Image:1wor.gif|left|200px]]<br /><applet load="1wor" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1wor, resolution 1.95&Aring;" />
 
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'''Crystal Structure of T-protein of the Glycine Cleavage System'''<br />
 
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==Overview==
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==Crystal Structure of T-protein of the Glycine Cleavage System==
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The glycine cleavage system catalyzes the oxidative decarboxylation of, glycine in bacteria and in mitochondria of animals and plants. Its, deficiency in human causes nonketotic hyperglycinemia, an inborn error of, glycine metabolism. T-protein, one of the four components of the glycine, cleavage system,is a tetrahydrofolate dependent aminomethyltransferase. It, catalyzes the transfer of the methylene carbon unit to tetrahydrofolate, from the methylamine group covalently attached to the lipoamide arm of, H-protein. To gain insight into the T-protein function at the molecular, level, we have determined the first crystal structure of T-protein from, Thermotoga maritima by the multiwavelength anomalous diffraction method of, x-ray crystallography and refined four structures: the apoform; the, tetrahydrofolate complex; the folinic acid complex; and the lipoic acid, complex. The overall fold of T-protein is similar to that of the, C-terminal tetrahydrofolate-binding region (residues 421-830) of, Arthrobacter globiformis dimethylglycine oxidase. Tetrahydrofolate (or, folinic acid) is bound near the center of the tripartite T-protein. Lipoic, acid is bound adjacent to the tetrahydrofolate binding pocket, thus, defining the interaction surface for H-protein binding. A homology model, of the human T-protein provides the structural framework for understanding, the molecular mechanisms underlying the development of nonketotic, hyperglycinemia due to missense mutations of the human T-protein.
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<StructureSection load='1wor' size='340' side='right'caption='[[1wor]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1wor]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WOR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WOR FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=RED:DIHYDROLIPOIC+ACID'>RED</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wor FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wor OCA], [https://pdbe.org/1wor PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wor RCSB], [https://www.ebi.ac.uk/pdbsum/1wor PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wor ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GCST_THEMA GCST_THEMA] The glycine cleavage system catalyzes the degradation of glycine (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wo/1wor_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wor ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1WOR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima] with RED as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Aminomethyltransferase Aminomethyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.2.10 2.1.2.10] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1WOR OCA].
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*[[Aminomethyltransferase 3D structures|Aminomethyltransferase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of T-protein of the glycine cleavage system. Cofactor binding, insights into H-protein recognition, and molecular basis for understanding nonketotic hyperglycinemia., Lee HH, Kim DJ, Ahn HJ, Ha JY, Suh SW, J Biol Chem. 2004 Nov 26;279(48):50514-23. Epub 2004 Sep 7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15355973 15355973]
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[[Category: Large Structures]]
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[[Category: Aminomethyltransferase]]
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[[Category: Single protein]]
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[[Category: Thermotoga maritima]]
[[Category: Thermotoga maritima]]
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[[Category: Ahn, H.J.]]
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[[Category: Ahn HJ]]
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[[Category: Ha, J.Y.]]
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[[Category: Ha JY]]
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[[Category: Kim, D.J.]]
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[[Category: Kim DJ]]
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[[Category: Lee, H.H.]]
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[[Category: Lee HH]]
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[[Category: Suh, S.W.]]
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[[Category: Suh SW]]
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[[Category: RED]]
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[[Category: aminomethyltransferase]]
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[[Category: dihydrolipoic acid]]
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[[Category: t-protein]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 05:34:05 2007''
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Current revision

Crystal Structure of T-protein of the Glycine Cleavage System

PDB ID 1wor

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