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1wps

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(New page: 200px<br /><applet load="1wps" size="450" color="white" frame="true" align="right" spinBox="true" caption="1wps, resolution 2.80&Aring;" /> '''Crystal Structure of...)
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[[Image:1wps.gif|left|200px]]<br /><applet load="1wps" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1wps, resolution 2.80&Aring;" />
 
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'''Crystal Structure of HutP, an RNA binding anti-termination protein'''<br />
 
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==Overview==
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==Crystal Structure of HutP, an RNA binding anti-termination protein==
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HutP regulates the expression of the hut structural genes of Bacillus, subtilis by an anti-termination mechanism and requires two components, Mg2+ ions and L-histidine. HutP recognizes three UAG triplet units, separated by four non-conserved nucleotides on the terminator region. Here, we report the 1.60-A resolution crystal structure of the quaternary, complex (HutP-L-histidine-Mg2+-21-base single-stranded RNA). In the, complex, the RNA adopts a novel triangular fold on the hexameric surface, of HutP, without any base-pairing, and binds to the protein mostly by, specific protein-base interactions. The structure explains how the HutP, and RNA interactions are regulated critically by the l-histidine and Mg2+, ion through the structural rearrangement. To gain insights into these, structural rearrangements, we solved two additional crystal structures, (uncomplexed HutP and HutP-L-histidine-Mg2+) that revealed the, intermediate structures of HutP (before forming an active structure) and, the importance of the Mg2+ ion interactions in the complexes.
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<StructureSection load='1wps' size='340' side='right'caption='[[1wps]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1wps]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WPS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WPS FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wps FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wps OCA], [https://pdbe.org/1wps PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wps RCSB], [https://www.ebi.ac.uk/pdbsum/1wps PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wps ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/HUTP_BACSU HUTP_BACSU] Antiterminator that binds to cis-acting regulatory sequences on the mRNA in the presence of histidine, thereby suppressing transcription termination and activating the hut operon for histidine utilization.[HAMAP-Rule:MF_00779]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wp/1wps_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wps ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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HutP regulates the expression of the hut structural genes of Bacillus subtilis by an anti-termination mechanism and requires two components, Mg2+ ions and L-histidine. HutP recognizes three UAG triplet units, separated by four non-conserved nucleotides on the terminator region. Here we report the 1.60-A resolution crystal structure of the quaternary complex (HutP-L-histidine-Mg2+-21-base single-stranded RNA). In the complex, the RNA adopts a novel triangular fold on the hexameric surface of HutP, without any base-pairing, and binds to the protein mostly by specific protein-base interactions. The structure explains how the HutP and RNA interactions are regulated critically by the l-histidine and Mg2+ ion through the structural rearrangement. To gain insights into these structural rearrangements, we solved two additional crystal structures (uncomplexed HutP and HutP-L-histidine-Mg2+) that revealed the intermediate structures of HutP (before forming an active structure) and the importance of the Mg2+ ion interactions in the complexes.
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==About this Structure==
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Structural basis of HutP-mediated anti-termination and roles of the Mg2+ ion and L-histidine ligand.,Kumarevel T, Mizuno H, Kumar PK Nature. 2005 Mar 10;434(7030):183-91. PMID:15758992<ref>PMID:15758992</ref>
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1WPS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1WPS OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structural basis of HutP-mediated anti-termination and roles of the Mg2+ ion and L-histidine ligand., Kumarevel T, Mizuno H, Kumar PK, Nature. 2005 Mar 10;434(7030):183-91. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15758992 15758992]
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</div>
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<div class="pdbe-citations 1wps" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Kumar, P.K.R.]]
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[[Category: Kumar PKR]]
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[[Category: Kumarevel, T.S.]]
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[[Category: Kumarevel TS]]
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[[Category: Mizuno, H.]]
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[[Category: Mizuno H]]
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[[Category: antitermination]]
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[[Category: hutp]]
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[[Category: rna binding]]
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[[Category: transcription regulation]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 05:35:29 2007''
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Current revision

Crystal Structure of HutP, an RNA binding anti-termination protein

PDB ID 1wps

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