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2w8d
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Distinct and essential morphogenic functions for wall- and lipo- teichoic acids in Bacillus subtilis== | |
| + | <StructureSection load='2w8d' size='340' side='right'caption='[[2w8d]], [[Resolution|resolution]] 2.35Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[2w8d]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W8D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2W8D FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr> | ||
| + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=TPO:PHOSPHOTHREONINE'>TPO</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2w8d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w8d OCA], [https://pdbe.org/2w8d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2w8d RCSB], [https://www.ebi.ac.uk/pdbsum/2w8d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2w8d ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/LTAS2_BACSU LTAS2_BACSU]] Catalyzes the polymerization of lipoteichoic acid (LTA) polyglycerol phosphate, a reaction that presumably uses phosphatidylglycerol (PG) as substrate.<ref>PMID:17483484</ref> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w8/2w8d_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2w8d ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Teichoic acids (TAs) are anionic polymers that constitute a major component of the cell wall in most Gram-positive bacteria. Despite decades of study, their function has remained unclear. TAs are covalently linked either to the cell wall peptidoglycan (wall TA (WTA)) or to the membrane (lipo-TA (LTA)). We have characterized the key enzyme of LTA synthesis in Bacillus subtilis, LTA synthase (LtaS). We show that LTA is needed for divalent cation homoeostasis and that its absence has severe effects on cell morphogenesis and cell division. Inactivation of both LTA and WTA is lethal and comparison of the individual mutants suggests that they have differentiated roles in elongation (WTA) and division (LTA). B. subtilis has four ltaS paralogues and we show how their roles are partially differentiated. Two paralogues have a redundant role in LTA synthesis during sporulation and their absence gives a novel absolute block in sporulation. The crystal structure of the extracytoplasmic part of LtaS, solved at 2.4-A resolution, reveals a phosphorylated threonine residue, which provides clues about the catalytic mechanism and identifies the active site of the enzyme. | ||
| - | + | Distinct and essential morphogenic functions for wall- and lipo-teichoic acids in Bacillus subtilis.,Schirner K, Marles-Wright J, Lewis RJ, Errington J EMBO J. 2009 Apr 8;28(7):830-42. Epub 2009 Feb 19. PMID:19229300<ref>PMID:19229300</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 2w8d" style="background-color:#fffaf0;"></div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Vibrio subtilis ehrenberg 1835]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Errington, J]] | ||
| + | [[Category: Lewis, R J]] | ||
| + | [[Category: Marles-Wright, J]] | ||
| + | [[Category: Schirner, K]] | ||
| + | [[Category: B subtili]] | ||
| + | [[Category: Cell membrane]] | ||
| + | [[Category: Cell wall]] | ||
| + | [[Category: Cell wall biogenesis/degradation acid]] | ||
| + | [[Category: Lipotechoic acid]] | ||
| + | [[Category: Lta]] | ||
| + | [[Category: Membrane]] | ||
| + | [[Category: Phosphatase]] | ||
| + | [[Category: Secreted]] | ||
| + | [[Category: Transferase]] | ||
| + | [[Category: Transmembrane]] | ||
| + | [[Category: Wta]] | ||
Current revision
Distinct and essential morphogenic functions for wall- and lipo- teichoic acids in Bacillus subtilis
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