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1wq2
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(New page: 200px<br /><applet load="1wq2" size="450" color="white" frame="true" align="right" spinBox="true" caption="1wq2, resolution 2.4Å" /> '''Neutron Crystal Struc...) |
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| - | [[Image:1wq2.gif|left|200px]]<br /><applet load="1wq2" size="450" color="white" frame="true" align="right" spinBox="true" | ||
| - | caption="1wq2, resolution 2.4Å" /> | ||
| - | '''Neutron Crystal Structure Of Dissimilatory Sulfite Reductase D (DsrD)'''<br /> | ||
| - | == | + | ==Neutron Crystal Structure Of Dissimilatory Sulfite Reductase D (DsrD)== |
| - | Dissimilatory sulfite reductase D (DsrD) from Desulfovibrio vulgaris has | + | <StructureSection load='1wq2' size='340' side='right'caption='[[1wq2]], [[Resolution|resolution]] 2.40Å' scene=''> |
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[1wq2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Desulfovibrio_vulgaris Desulfovibrio vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WQ2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WQ2 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Neutron Diffraction, [[Resolution|Resolution]] 2.4Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DOD:DEUTERATED+WATER'>DOD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wq2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wq2 OCA], [https://pdbe.org/1wq2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wq2 RCSB], [https://www.ebi.ac.uk/pdbsum/1wq2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wq2 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/DSVD_DESVH DSVD_DESVH] May play an essential role in dissimilatory sulfite reduction. | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wq/1wq2_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wq2 ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Dissimilatory sulfite reductase D (DsrD) from Desulfovibrio vulgaris has been crystallized for a neutron diffraction study. The initial crystals obtained were too small for the neutron experiment. In order to obtain a larger crystal (>1 mm3), a combination of two techniques was developed to determine the optimum crystallization conditions: a crystallization phase diagram was obtained, followed by crystal-quality assessment via X-ray diffraction. Using conditions determined in this manner, a large single crystal (1.7 mm3) of DsrD protein was subsequently grown in D(2)O solution by the macroseeding technique. A neutron diffraction experiment was carried out using the BIX-3 diffractometer at the Japan Atomic Energy Research Institute (JAERI), collecting data to 2.4 A resolution from an optimized crystal. | ||
| - | + | Crystallization and preliminary neutron analysis of the dissimilatory sulfite reductase D (DsrD) protein from the sulfate-reducing bacterium Desulfovibrio vulgaris.,Chatake T, Mizuno N, Voordouw G, Higuchi Y, Arai S, Tanaka I, Niimura N Acta Crystallogr D Biol Crystallogr. 2003 Dec;59(Pt 12):2306-9. Epub 2003, Nov 27. PMID:14646103<ref>PMID:14646103</ref> | |
| - | + | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| + | <div class="pdbe-citations 1wq2" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Desulfovibrio vulgaris]] | [[Category: Desulfovibrio vulgaris]] | ||
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: Arai | + | [[Category: Arai S]] |
| - | [[Category: Chatake | + | [[Category: Chatake T]] |
| - | [[Category: Higuchi | + | [[Category: Higuchi Y]] |
| - | [[Category: Mizuno | + | [[Category: Mizuno N]] |
| - | [[Category: Niimura | + | [[Category: Niimura N]] |
| - | [[Category: Tanaka | + | [[Category: Tanaka I]] |
| - | [[Category: Voordouw | + | [[Category: Voordouw G]] |
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Current revision
Neutron Crystal Structure Of Dissimilatory Sulfite Reductase D (DsrD)
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